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...honestly evaluate your own style of work and communication before making this important decision. I’ll ...Prepare by going sleuthing on the lab website, publication records, LinkedIn, ResearchGate or Twitter. Be...the appropriate first authorship position on a publication of her project and made another lab member first...– including failure to acknowledge good work, allocation of meaningless tasks, removal of responsibility...connections such as serving on Boards? Does the publication record show evidence of industry collaboration...skills guide Learn about careers in science communication Read posts on mentoring for scientists Resources...

...piggyBac™ technology can be used for numerous applications, including transgenesis, gene-trap screens, ...of the piggyBac™  transposase/transposon gene modification system and the scarless, Footprint-Free™ removal...technology that may be used for a variety of research applications, including making transgenic cell lines and ...PMCID: PMC2567143. 6. S. Radecke, F. Radecke, T. Cathomen, and K. Schwarz, “Zinc-finger nuclease-induced...oligodeoxynucleotides: wanted and unwanted target locus modifications.,” Mol. Ther., vol. 18, no. 4, pp. 743–53, Apr...Addgene.org Find Vectors with the piggyBac™ Transposon Catch up on Everything CRISPR with Our CRISPR Guide Deposit...

...speak for you (and you for them) Communication Being able to communicate effectively is part of what makes...you can. The colleagues you help will be future advocates and references. Collaborate with another lab,...productive. Take all the opportunities you can to be communicating about science with others. If opportunities ... (be on a speaker committee) Follow industry publications like FierceBiotech to “learn the business” Attend...

...results, modifications, and inventions created by the Recipient Right to file patent applications on modifications... about intellectual property rights, pending publications, and commercial use often come up. An MTA can...trials Acknowledgement in recipient’s future publications relating to the material Limited liability...

...Since the first research applications of GFP were published in the 1990s, biologists have spent a lot...color was observed after excitation at 488 nm, indicating that spontaneous conversion did not occur. Tobacco... Sattarzadeh et al., Sci Rep. 2015 CC BY 4.0 Applications and limitations of GFP photoconversion One concern...2):131-3.  PubMed. Find plasmids from this publication at Addgene. Subach OM, Patterson GH, Ting LM...nmeth.1664. PubMed. Find plasmids from this publication at Addgene.   Addgene’s Fluorescent Protein...

...working in your chosen field. This cooperative education experience (co-op) is what has brought me, a second... QC/QA team as a DNA Sequencing Intern. The application process When I first saw the comprehensive job...for the intended position. After submitting my application, I was contacted to participate in a brief fifteen...the depositing labs intended. With the use of applications like SnapGene and Geneious, I was able to determine...currently working on projects for the Science Communication function at Addgene. Working a 9-5 As it turned...

...mostly because of this article: “A systematic identification and analysis of scientists on Twitter.” This...from the United States Standard Occupational Classification System  and Wikipedia. 2. Social and computer...: Portion of Tweets with Scientific Domains, Categorized by Discipline. Biologists, neuroscientists, and... Y., & Sugimoto, C. R. (2017). A systematic identification and analysis of scientists on Twitter. Plos...Scientists 5 Reasons to Use Reddit for Science Communication 10 Ways to Share Your Science Why Scientists...

...adaptive bacterial immunity and its potential applications in rewriting the genome. Their award acceptance... same Polish sub-dialect making their Skype communications extra fun) who shared first authorship on their...simpler 1 protein - 1 RNA system.  Dr. Charpentier advocated for the crucial role of basic research in the ... area will accelerate the launch of clinical applications. During the panel discussion, Dr. Mello took...for billions of years (…) Bacteria are really sophisticated”.     New to CRISPR/Cas9 Technology? Learn ...

...of hues. While those tools are great for many applications, Verkhusha and his lab at Albert Einstein College...light. As the researchers described in Nature Communications last year, “The capability to control spectral...Verkhusha’s team reported another advance in the application of near-infrared fluorescent proteins, iRFP670...fluorescence is because the light becomes very scattered,” Verkhusha explained. Ultrasound wavelengths,...hand, are larger than the size of a cell, so they scatter much less. As a result, ultrasound enables higher...

... difficult to eradicate. You can suppress the growth of mycoplasma but cannot eradicate it completely....Contamination! Tissue culture media often have indicators included in the media that turns yellow from ...important to prevent contamination rather than eradicating it later. Don’t be scared when mycoplasma infects...the first person to face this kind of problem. Educate others when you can and help them understand the...Cc, Drexler Hg (2002) Comparative Antibiotic Eradication of Mycoplasma Infections from Continuous Cell...

...transfer expert who understands the somewhat complicated Material Transfer Agreement process that kits...most scientific collaborations fall within this category. Unlike a process team, you don’t necessarily ...functioning (or malfunctioning). Clear lines of communication Few people actually like meetings, but run well...are helpful, they are certainly not the only communication options. Addgene, along with an increasing number...desks away or on the other side of the world, communication platforms like Slack make teamwork easier. Trust...excellent leader, and perfectly clear lines of communication, but if teammates don’t trust each other, none...

...tackling these particular tasks, but they tend to be scattered about the internet. To help you keep your virtual...: Find sequences for plasmids assembled from publications and commercially available sources, as well ... as expression patterns, post-translational modifications and polymorphisms Codon optimization tool: To...making it ready to copy and paste into other applications Primer design tools Many of the above-listed... well as primer design for different cloning applications. Primer3 and Primer3Plus: For general primer...

...compounds, links to publication references, and cross-references with cell line catalogs and collections....surveying of cell line collections websites and catalogues Literature searches utilizing Google Scholar ...include accession number, species, and assigned category (cancerous, hybridoma, etc.), as well as sex and...manually going through the pdfs of the 26,000 publications cited. If the information seems incomplete or...

...composed of camels, llamas, and alpacas (and their undomesticated counterparts) - not exactly species that jump...to sdAbs becoming valuable tools for diverse applications. For example, RANbodies can be used as an alternative... excitement around using sdAbs in diagnostic applications. For example, fluorescently and radioactively...heavy-chain only antibodies. (A) Overview of genetic modifications made to generate the nanomouse. (VHH = variable...inspired this versatile family of tools, the applications they have already enabled, and the discoveries...

...job - we strive to also give you high-quality educational resources to help you do better research. CRISPR...design, sorting the plasmids we have available by category. Each plasmid page now has a helpful graphic to... newest page in the Transcriptional Regulation category already has five blue flame plasmids covering ...providing an avenue for temporary/reversible CRISPR modification. In addition to these subpages, our updated ... HypaCas9 Day 10: Transcriptional regulation: Truncated gRNAs for Regulating Gene Expression and CRISPR...

...scientists work on projects spanning from the identification of plant compounds and insecticidal proteins...bacteria. Learn more about the different CRISPR applications in plants.     Transgenic yeast as source of... yeast capable of desaturating fatty acids. Identification of Insecticidal Proteins Linh Bao Ton (M. Sc...Arturo Rivera Solís, Universidad Autónoma de Yucatán, Mexico Dr. Rodrigo Arturo Rivera Solís, Associate...wide range of research fields and geographical locations. We are excited to see how all of the Addgene-...

...of any scientist’s career. We use writing to communicate our findings and share them with the world. If...simply writing down your data doesn’t guarantee a publication. There is an art to composing manuscripts and...field of microbiology with multiple high-profile publications. My classmates consisted of graduate students...well as being critiqued added an extra level of application not present in the online class.   Four ways ...paper will be generated. After you complete a publication quality figure, produce the figure, its legend...

... Specifically, viruses are able to infect and replicate in host cells, but they are susceptible to cells... though not all, of these defenses. For some applications of genome engineering - such as certain forms...to integrate. Additionally, the TA sequence is duplicated near the gene of interest insertion site.   The...1) an SB transposase, the enzyme required for catalysis of transposition; and 2) a transposon containing...containing the gene-expression cassette that can translocate within the genome. The Sleeping Beauty transposase...

... (e.g. 6xHis containing pDEST-HisMBP). After purification the protein can be released from the resin and...types of yeast expression vectors. Consider your applications and experimental design. You can create a new...more traditional approach of an autonomously replicating vector that carries either a wild-type version...a variety of other types of vectors for most applications used to study gene function. From making transgenic...transgenic animals, gene targeting and genome modifications to reporter vectors (e.g. pBV-Luc) and gene ...

...distinct types and subtypes using polythetic classification based on evolutionary similarity (5). Type ...2013 (7, 8). Nine months later, more than 1500 publications describe work to improve the tool’s specificity...various species and describe an assortment of applications. Despite the ubiquity of CRISPR/Cas toolkit,... multiple plasmids used for various platform applications. For more information on CRISPR/Cas, check out...for your research. Do you have a cool idea or application that involves CRISPR/Cas technology? Let us know...