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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 804 results for: cha

Showing: 381 - 400 of 804 results

  2. Inntags: Innovative Protein Epitope Tagging

    Type
    Blog Post
    Published
    Nov. 10, 2015, 3:30 p.m.
    ...created tagged versions of the cyclin Cdc28 and the chaperone Ydj1, both of which display sophisticated interactions...with multiple other proteins. Again, little to no change in cell morphology was observed with IT5 and IT6...methods (2015). PubMed PMID:26322837. 2. Stadler, Charlotte, et al. "Immunofluorescence and fluorescent-protein...

  3. CRISPR-Cas14: a family of small DNA-targeting enzymes enabling high-fidelity SNP genotyping

    Type
    Blog Post
    Published
    Nov. 29, 2018, 1:53 p.m.
    ...Department of Energy’s Joint Genome Institute for uncharacterized genes that were proximal to a CRISPR array ...presence of a predicted RuvC nuclease domain, characteristic of CRISPR-Cas enzymes. In contrast to other...bacterial genomes but only in the genome of a group of Archaea. Consistent with this origin, the authors suggest...

  4. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    Published
    Aug. 4, 2015, 2:30 p.m.
    ...biologists have spent a lot of time making things glow. Chances are you’ve used a GFP derivative to conduct subcellular...optogenetics. Even though GFP has been so thoroughly characterized, it turns out this protein has a few more secrets... LM, Wang Y, Condeelis JS, Verkhusha VV. A photoswitchable orange-to-far-red fluorescent protein, PSmOrange...

  5. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type
    Blog Post
    Published
    Feb. 18, 2015, 3:09 p.m.
    ... inferred based on correlative biochemical characteristics and ectopic reporter assays. We have been interested..., identifying these deletions, and finally characterization. Read and download the full protocol from ...Dass A, Yien YY, Chung J, Masuda T, et al. Characterization of genomic deletion efficiency mediated by...

  6. Inside Addgene's 10 Year Anniversary Gala

    Type
    Blog Post
    Published
    July 3, 2014, 3:48 p.m.
    ... desserts. We enjoyed a video highlighting the changes at Addgene over the last 10 years as well as the...fruition. The concept, though relatively simple, changed the course of scientific research. What was once... ghost writers, of course! Amy Uddin and Emma Gottschall, two of Addgene's lab technicians, gave Blugene...

  7. "What Makes a Good Mentor?" and 6 More FAQs About Science Mentoring

    Type
    Blog Post
    Published
    Dec. 17, 2013, 2:47 p.m.
    ...Navigating a fulfilling career in science can be challenging – is advice and guidance from only 2 people enough...with the researchers who joined a recent Twitter chat for Early Career Researchers.  Many of the participants...lighthearted, the article does highlight key characteristics of a good mentor: Yoda image courtesy...

  8. Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry

    Type
    Blog Post
    Published
    June 23, 2020, 1:15 p.m.
    ...                      University of North Carolina at Chapel Hill University of Illinois Chicago                     ...beyond academia. For example: Plasmids from the Michael J. Fox Foundation are used in Parkinson’s research...carriers (SLCs) for medical research Recently, the Michael Davidson Fluorescent Protein collection, a ‘One-Stop...

  9. Three Key Considerations for Precise Neuronal Targeting Using AAV Technologies

    Type
    Blog Post
    Published
    Nov. 27, 2019, 2:03 p.m.
    ...development, learning, and behavior poses many challenges in neuroscience with many brain regions, neuronal...specific neuronal subtypes and determine how these changes affect the phenotype. Viral vectors and genetic...specific cell type. Find some transduction characteristics of select AAV serotypes in Table 2 from the...

  10. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    Published
    Sept. 28, 2020, noon
    ...homologous recombination in bacteria. These quirks change the way CRISPR-mediated genome engineering functions...While the activation of transcription is a more challenging problem than repression, scientists have deposited...the target sequence which could be adjusted by changing the sgRNA length. The system could be further ...Streptomyces, yet genome engineering remains challenging for most organisms in the genus. Work from the...

  11. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type
    Blog Post
    Published
    June 19, 2018, 1:38 p.m.
    ...widely available imaging platform: ultrasound. Challenges of in vivo imaging with existing bacterial reporters... by water-dwelling photosynthetic bacteria and archaea to regulate their buoyancy. The Shapiro lab recently...genes for in vivo imaging Following in vitro characterization, Bourdeau et al performed a proof-of-concept...

  12. We're Updating Our Hold for Publication Policy

    Type
    Blog Post
    Published
    June 24, 2025, 1:15 p.m.
    ...are distributed. Next month, we are making two changes to the policy that will make the process a bit ... the published-paper-celebration checklist as champagne or cupcakes, or maybe the lab member originally...updating our Hold for Publication policy with two changes that will help us ensure that plasmids don’t get...

  13. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

    Type
    Blog Post
    Published
    Sept. 12, 2023, 1:15 p.m.
    ...Sometimes DNA sequences need to be “cleaned up” or changed in some way before you can analyze them. These ...complement sequence.  Filter DNA tool: Removes non-DNA characters, such as numbers and blank spaces, from the text... primer dimer estimation, and other primer characteristics. NEB Data-optimized Assembly Design (DAD): ...

  14. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    Published
    July 27, 2021, 1:15 p.m.
    ...provide new opportunities for investigating genetic mechanisms related to morphogenesis, cell fate specification...enzymatic receptor DAD2. SLs induce a conformational change of the receptor which modulates the fluorescence... a major economic impact.   Design and mechanism of action of the receptor-based SL fluorescent...

  15. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    Published
    June 4, 2020, 1:22 p.m.
    ...backbone of the DNA helix or even changes the DNA sequence through a mechanism called depurination. In depurination...contamination.  Extreme pH can denature, break, and even change the DNA sequence. High pH favors the breakage of...

  16. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    Published
    April 2, 2020, 6:17 p.m.
    ...found in bacteria, but also exist naturally in archaea and eukaryotes such as yeast and plants. In nature...manipulate gene expression in target cells. Characteristics such as flexibility, versatility, safety, and...Produce enzymes that will make specific, controlled changes to an organism’s genome (genome engineering) Produce...

  17. Management for Scientists: Giving Feedback

    Type
    Blog Post
    Published
    Feb. 6, 2015, 2:46 p.m.
    ... for giving feedback Use formal and informal mechanisms for feedback (more on this below). Make sure...positive outcome if the employee sees that real change is possible in your eyes so their confidence is...feedback is a very effective way to bring about change in behaviors and habits in a nonthreatening way...

  18. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    Published
    Dec. 9, 2014, 2:22 p.m.
    ... of freedom and diversity built into their jobs Chance to do/learn new things, diversity of responsibilities...it, we are special. Below are some special characteristics of scientists in the work place as described...identify the work style of each team member) Need challenging work, opportunities to pursue and problems to...

  19. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    Published
    March 26, 2019, 1:40 p.m.
    ...single-wavelength sensors with intensities that change in response to calcium binding. There are a few...AAV5  AAV SYN flex PSAM4 GlyR IRES EGFP Chimeric channels for neuronal manipulation 119742  AAV5  AAV ...AAV SYN PSAM4 GlyR IRES EGFP Chimeric channels for neuronal manipulation Recombinases Plasmid Serotype...

  20. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    Published
    Jan. 22, 2019, 2:41 p.m.
    ...for the measurement of autophagic flux, or the changes in autophagic activity, and are a more reliable...autophagy-related microtubule-associated proteins 1A/1B light chain 3B, or LC3. LC3 is a commonly used marker for measuring...functions as an autophagy bionsensor via the same mechanism as Rosella: GFP is quenched in acidic environments...
Showing: 381 - 400 of 804 results