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We narrowed to 763 results for: ire;

Showing: 381 - 400 of 763 results

  1. Viral Vectors 101: Transductions

    Type
    Blog Post
    Published
    April 30, 2024, 1:15 p.m.
    ...cells by any means, but are unique in that they require delivery to be viral-mediated. Transformations ...retrovirus, lentivirus, adenovirus, or AAV. Then, your desired DNA to be integrated can be cloned into the vector...weeks, depending on the virus, cell type, and/or desired event.   Figure 1: Transduction to expression...

  2. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    Published
    April 25, 2019, 3:01 p.m.
    ... Using structural modeling and structure-guided direct evolution, Nathan Shaner and colleagues from The...violet range. For example, mWasabi can be used with Sapphire (excitation maximum 399 nm) because it does not... at Addgene! References Ai, Hui-wang, et al. "Directed evolution of a monomeric, bright and photostable...

  3. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type
    Blog Post
    Published
    Sept. 17, 2020, 1:15 p.m.
    ...operations for all ongoing processes are entirely self-supporting. We hire more staff as we need them to meet... were closed and not receiving packages. So the entire team mobilized to figure out how to deliver when...

  4. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type
    Blog Post
    Published
    June 2, 2020, 1:15 p.m.
    ...rate discovery rate, conventional CRISPR screens require a low multiplicity of infection (MOI) during cell... tools for doing this were labor-intensive and required using manipulations such as injection of precise...drives gene expression. Because calcineurin is required for virulence in pathogenic fungi, it is an attractive...

  5. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    Published
    March 5, 2020, 2:15 p.m.
    ...using AAV on our blog. Image: The final and required step requires submission of an experimental image result...expression of AAV cargo? Did you use GCaMP expression? Direct Fluorescence? Immunohistochemistry? Did you use...

  6. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    Published
    Dec. 19, 2019, 2:40 p.m.
    ...in the last few months. You can also browse our entire AAV inventory. Our new AAVs include: Optogenetics... See our Serotype Testing AAV inventory for the entire collection! Plasmid Serotype Name 51502 AAV5... sensors provide the spatiotemporal resolution required for precise in vivo measurements of dopamine (...

  8. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    Published
    Jan. 21, 2014, 3:08 p.m.
    ...communication. A great approach to fully understand and acquire competitive skill sets in a myriad of professions...job sites to find open positions and view their required qualifications and skill sets Taking the time...graduate students and postdocs are currently getting hired? Let us know what topic you'd like to learn more...

  9. 5 Great Apps for Lab Life

    Type
    Blog Post
    Published
    Sept. 22, 2016, 2:30 p.m.
    .... The tools are a lifesaver when your research requires cell culture and include a counter, lab timer,...Tools app comes in handy when I use protocols that require restriction enzymes or PCR. The app contains a ...the app is quite helpful and provides a list of directions including which buffers to use and that indicates...

  10. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type
    Blog Post
    Published
    April 28, 2021, 1:15 p.m.
    ... Just enter the DOI of a paper and select your desired formatting style. This tool is great for generating...with others. The Web Importer plug-in lets you directly import papers from web pages and academic databases...the Zotero Connector, papers can be saved from Firefox or Chrome browsers with just one click. Papers ...

  11. Cultivating Community Science at BosLab

    Type
    Blog Post
    Published
    Nov. 2, 2017, 1:35 p.m.
    ...BosLab and previous laboratory experience is not required. Our members are skilled with audiovisual media...initiated a soil microbiome project. This project was inspired by previous city-wide genomic studies and our ...DIY bio If you’re not in the Boston area but are inspired to get involved, check out the local groups page...

  12. How to Lead a Great Meeting

    Type
    Blog Post
    Published
    Feb. 9, 2016, 3:30 p.m.
    ...scope of the meeting: Are you meeting to design an entire experiment and delegate tasks to lab mates or to... to discuss a potential new research direction you’d like to pursue? Undefined scope can lead to long ... Get Management Advice from Addgene Executive Director Joanne Kamens FInd Tips on Picking a Research ...

  13. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    Published
    Aug. 6, 2024, 1:15 p.m.
    ...tropism. Briefly, different envelope plasmids can direct the production of virus with various tropisms.  ...therefore, reduces the number of plasmids that are required in the transfection step of the viral packaging...incompetent? Yes, as with lentiviruses, the genes required for γ-retroviral packaging and transduction are...

  15. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ...catch our April AAV webinar with Tim Miles, PhD, Director of the CLOVER Center at CalTech? If so, you may...trans-protein splicing. Note, however, that this will require co-delivery of two AAVs at a high MOI, often resulting...1x1012 vg/mouse is more standard) and reflects a desire for very high MOI on top of very high coverage....

  16. Hot Plasmids - February 2022

    Type
    Blog Post
    Published
    Feb. 22, 2022, 2:15 p.m.
    ...label your favorite protein! While the target only requires a small epitope tag (FLAG is eight amino acids... is advantageous to Cas9’s larger expression requirements (Figure 1), but Cas12a has generally been underutilized...when encoding multiple guides. While two guides require two arrays for Cas9, two guides can be streamlined...

  17. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    Published
    Feb. 8, 2022, 2:15 p.m.
    ...Passion? A love of learning? No. Or at least not entirely. What truly unites us all is the frustration of... to the day before, which got rid of the issue entirely. In many cases, the answer isn't to work harder...ability to focus, especially on days when I was tired, stressed, or rushing. I would spend the time quietly...

  18. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ...biological phenomena by generating an output which is directly proportionate to the amount of a given factor ...either factors as proxies for a cellular process or directly measure a cellular process. Cellular phenomena...type of cells the viral particles infect. This can direct your biosensor to specific tissues or cell (sub...

  19. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    Published
    March 21, 2023, 1:15 p.m.
    ...transfect’, such as primary cells. They can also be directly injected into hard-to-reach organs, such as the...like replication incompetent viral particles, are required. We will be covering these safety measures in ...dividing cells, as their access to the host genome requires breakdown of the nuclear envelope, which occurs...

  20. How Addgene Creates Content

    Type
    Blog Post
    Published
    Nov. 17, 2022, 2:15 p.m.
    ...truly believes that sharing speeds science, and our entire mission is to enable that process. Our repository... made most of the Antibody Guide figures. This requires a fair amount of communication between our content...of date as techniques and knowledge changes and require updating or retiring. CRISPR/Cas9, for example,...
Showing: 381 - 400 of 763 results