We narrowed to 829 results for: lars

...Lichtman, both of Harvard’s Department of Molecular and Cellular Biology, harnessed these attributes of ...complementary Flpbow construct that is functionally similar to Cre-based Brainbow, but is controlled by FLP...as connections between motor neurons and the neuromuscular junction. In zebrafish, the method has become...neural expression patterns. Hampel S, Chung P, McKellar CE, Hall D, Looger LL, Simpson JH. Nature Methods...

...therefore determines the timing of gene expression and largely defines the amount of recombinant protein that ...cumate-inducible gene expression system, which functions in a similar manner to the Tet system. The transactivator configuration...interrogate the activity of uncharacterized promoters. Similarly, placing UAS upstream of a transgene permits directed... binary systems above if they all behave very similarly?” The simple answer is that sometimes it is necessary...systems. Recent work has even used all 3 in a single modular vector for ease of use! Conclusion This post contains...

...hard work and talent for many years (at a very low salary I might add). You have a right and responsibility...are always traveling, or too busy to interact regularly with the newer lab members. You must get an idea... the lab head? Are meetings with the lab head regularly scheduled or only ad hoc? Does the mentor keep... and learn. Graduate students and postdocs in particular must care about this. The training you get now...at your data and suggest follow up experiments regularly? Can you work on some experiments of your own ...

...plasmid molar ratio you would like to use for your AAV packaging transfection. In my lab we regularly use ...requires transfecting three plasmids at specific molar ratios. To get these ratios right, you need to do... Vitro and In Vivo Use." Current Protocols in Molecular Biology (2007): 16-25. PubMed PMID: 18265393. ...

...Choice" series. Each month, I'll summarize the most popular post of the month and point out one or more additional... ranging from new tools you can use to monitor cellular perturbations to how CRISPR has made it much easier...surrounding anything to do with CRISPR, our most popular post in July (with over 700 views) was an update...showing that Cpf1 has lower off-target editing and similar on-target editing when compared to the most commonly...

...Bengyella’s work has focused on elucidating the molecular mechanisms behind interactions between crops like...around the world. Kwabena O. Duedu Dr. Duedu’s molecular biology work has focused on using various cloning...plasmids he acquires through Addgene to produce molecular biological tools like Taq polymerase, T7 polymerase...heavy focus on teaching. He hopes to teach his molecular biology students about the functions of these ...

... you only look at 2 or 3 pairwise comparisons. Similar to family-wise error, is the false discovery rate... to reduce false positive rates and control for large numbers of comparisons. The Bonferroni does this...arranging all calculated p values from smallest to largest and ranking them (1 being the smallest value). ...calculates the cut off for significance by finding the largest p value where its rank divided by the total number...positives/negatives, but they all generally yield a similar result and are appropriate to use if all sample...

...indicates that the amount of leaky expression can largely be attributed to the level of spontaneous recombination...) promoter for catecholamine neurons or glial fibrillary acidic protein (GFAP) for astrocytes it is important...tested in the researcher's system before beginning large scale experiments. This will help to ensure the ...10.1371/journal.pone.0076310 Burger C, Gorbatyuk OS, Velardo MJ, Peden CS, Williams P, Zolotukhin S, Reier PJ...Different Regions of the Central Nervous System. Molecular Therapy 10:302–317. https://doi.org/10.1016/j....

... Read the molecular biology reference Find plasmids for your research Brush up on molecular biology protocols...as pure as for other uses.   Cloning grade DNA Molecular cloning includes a set of techniques that are ...consequence, the preparations for this technique must be larger scale, to allow the extraction of enough plasmid...

...Toolbox: Free Online Molecular Biology Tools by Beth Kenkel There's a lot of free molecular biology tools out...post. 8. Chromoproteins: Colorful Proteins For Molecular Biology Experiments by Jennifer Tsang Chromoproteins...generate color! They've been adapted for many molecular biology methods such as allelic exchange, FRET...

...limited to particular model organisms or cell types. “This tool is really flexible and modular,” Konermann... vitro by a factor of 10 to 20. A flexible and modular optogenetics tool The researchers also showed just...novel mode of optogenetic control of endogenous cellular processes." The LITE system can now be applied...

...food on special occasions. However, employees regularly bring in baked goods and we have a few unique ...During Hack Days, teams at Addgene will forgo their regular To-Do lists (other than emergency items) and focus...United States, I look forward to gathering around a large table for a meal with my family. I am thankful for...things, and one is that I get to gather around a large table to eat with my Addgene family every day. Do...

...transformants. Addgene distributes several ready-to-use, modular plasmids, combining fluorescent tags, epitope tags.... John Pringle and Jürg Bähler have deposited a large collection of plasmids for genome modification in...at UNC Chapel Hill. Bähler et al.(2) describe a modular collection of plasmids for a wide variety of genome... to the collections featured above, many other modular yeast tagging systems have been developed in the...

...alpha-1a adrenergic receptor; nLightG contains circularly permuted green fluorescent protein (GFP), and...green fluorescent proteins. nLightR did perform similarly to GRABNE, and having both colors allows for increased...AR. Selectivity Norepinephrine and dopamine are similar molecules, and cross-reactivity is a very common...continues to work on further improvements. In particular, they’re focused on generating variants with ...

...can help reduce off-target activity. Deleting a large region of the genome by removing the sequence between...Transcript Multiplexing Plasmids: These plasmids are similar to the Csy4 polycistronic system described above...Addgene. 4. Albers, Joachim, et al. “A versatile modular vector system for rapid combinatorial mammalian... genome editing in monocot and dicot plants.” Molecular Plant 8(8) (2015): 1274-1284. PubMed PMID: 25917172.... 11. Cress, Brady F., et al. “CRISPathBrick: Modular Combinatorial Assembly of Type II-A CRISPR Arrays...

...DNA substitutions don’t fare much better: they largely abolished cleavage by both Cas9 and Cas12 (Kim ...reducing potential off-target sites in the genome. Similar on-target efficacy. For perfect sequence matches...kinds of tools. Location of DNA substitution Similar on-target efficacy Reduced off-target activity ...resources on the Addgene blog Build Your CRISPR Vocabulary A Needle in a Base-Stack: Cas9 Structural Biology...

...General Assembly has declared 2024 the International Year of Camelids. The declaration is intended to raise...antibodies in the serum of a camel and soon after found similar antibodies in llamas and alpacas (Figure 1). At... tissues. sdAbs can also be used to manipulate cellular functions, like in this early example we highlighted...

...control over neuronal activity. While they serve similar purposes, both techniques offer researchers different...have faster off kinetics and allow neurons to repolarize faster.  Chemogenetics does not offer the same...targeted to specific tissues, cell types or even subcellular regions of a neuron. Optogenetic approaches excel...with light accessible brains, such as zebrafish larvae. Mouse studies, however, require a permanent intracranial...

...researchers and ready access to stably archived large-scale datasets by computational biologists. ...genome-wide datasets, which are often obscured in large supplementary data files, BioGRID ORCS makes it ... interest. In addition, users interested in a particular disease, cell type or drug treatment can browse...used to search the entire CRISPR database for particular screens of interest.   Figure 3: Each ...

...Problems with Chip-seq ChIP-seq typically requires a large amount of input material, cells or tissue, to produce...with cell culture, because it is easy to harvest large quantities of cells. However, when working on specific...false-positives, as proteins may appear to be binding at particular genomic sites when in fact they are just non-...situations when it is technically challenging to collect large numbers of cells or when a high degree of signal...