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We narrowed to 811 results for: lars

Showing: 381 - 400 of 811 results

  1. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    Published
    June 11, 2020, 1:34 p.m.
    ...control over neuronal activity. While they serve similar purposes, both techniques offer researchers different...have faster off kinetics and allow neurons to repolarize faster.  Chemogenetics does not offer the same...targeted to specific tissues, cell types or even subcellular regions of a neuron. Optogenetic approaches excel...with light accessible brains, such as zebrafish larvae. Mouse studies, however, require a permanent intracranial...

  2. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

    Type
    Blog Post
    Published
    Jan. 21, 2020, 2:30 p.m.
    ...researchers and ready access to stably archived large-scale datasets by computational biologists. ...genome-wide datasets, which are often obscured in large supplementary data files, BioGRID ORCS makes it ... interest. In addition, users interested in a particular disease, cell type or drug treatment can browse...used to search the entire CRISPR database for particular screens of interest.   Figure 3: Each ...

  3. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    Published
    Feb. 13, 2018, 2:51 p.m.
    ...Problems with Chip-seq ChIP-seq typically requires a large amount of input material, cells or tissue, to produce...with cell culture, because it is easy to harvest large quantities of cells. However, when working on specific...false-positives, as proteins may appear to be binding at particular genomic sites when in fact they are just non-...situations when it is technically challenging to collect large numbers of cells or when a high degree of signal...

  4. ReFigure: Save Scientific Figures into Dashboards and Share Your Insights

    Type
    Blog Post
    Published
    Dec. 13, 2017, 3:20 p.m.
    ...Plasmids with Addgene Find Molecular Biology Protocols and Videos Find Other Molecular Biology Resources ...paper and then another figure in another paper. A large fraction of readers cover the same ground again ...allows you to make meaningful collections from a large section of open access life sciences data. You can...

  5. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    Published
    Dec. 12, 2017, 3:13 p.m.
    ...easy to navigate. We also updated some our most popular CRISPR blogs to keep them current. Here are some...plasmid class covered on that page. To help you find popular plasmids, we’ve also added four new plasmid pages...mammalian systems, bacteria, plants, and yeast. Popular cytosine base editor pCMV-BE3 has been requested...only the specified RNA target. Bonus: It displays similar on-target activity to siRNA, but with far fewer...

  6. Year of the Camelid: Antibody Style

    Type
    Blog Post
    Published
    May 9, 2024, 1:15 p.m.
    ...General Assembly has declared 2024 the International Year of Camelids. The declaration is intended to raise...antibodies in the serum of a camel and soon after found similar antibodies in llamas and alpacas (Figure 1). At... tissues. sdAbs can also be used to manipulate cellular functions, like in this early example we highlighted...

  7. Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene

    Type
    Blog Post
    Published
    July 2, 2015, 8:37 p.m.
    ...Addgene. This is particularly true in my position as an Outreach Scientist where I spend a large amount of my...pretty hefty bumps during my graduate training. I particularly think that too many young scientists are led...experience is key to my current position, I think it’s largely my work developing myself as a science communicator...

  8. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    Published
    Nov. 25, 2013, 10:12 p.m.
    ...clustered regularly interspaced palindromic repeats) Cas (CRISPR associated genes) system. This popular genome...polythetic classification based on evolutionary similarity (5). Type II CRISPR/Cas is the system that scientists...translatable to the other CRISPR subtypes. Still, the large and dynamic range of CRISPR systems between and ...

  9. Meet the New Addgene Board Members!

    Type
    Blog Post
    Published
    April 28, 2020, 1:14 p.m.
    ...talk about Z DNA that inspired her to major in molecular biology in college. She then pursued a PhD in ...not only got incredibly compelled by wet bench molecular research, but also became passionate about virology...of Viral Vectors Karen Guerin for the journal Molecular Therapy. Vandenberge is also the Grousbeck Family... Gene Therapy Center, Associate Director of the Ocular Genomics Institute, and an Associate Member at ...

  10. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    Published
    Jan. 22, 2019, 2:41 p.m.
    ...fluorescence allows for clear distinction between vacuolar/lysosomal and cytoplasmic Rosella. When fused ...biosensor is the GFP-LC3-RFP-LC3ΔG probe. It’s similar to the mRFP-GFP-LC3 Tandem Fluorescent Protein,...Rosella: a fluorescent pH-biosensor for reporting vacuolar turnover of cytosol and organelles in yeast." ...measuring autophagy." International journal of molecular sciences 18.9 (2017): 1865.  PubMed PMID: 28846632...

  11. Antibodies 101: Epitope Availability

    Type
    Blog Post
    Published
    Jan. 11, 2022, 2 p.m.
    ...If you work in a lab that regularly does immunoimaging, there’s likely a large collection of antibodies...specific to denatured versions, or even to just that particular assay.      Fig. 1: A native protein ...that has been validated for an assay that uses similar sample preparation - for instance, if you cannot...

  12. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

    Type
    Blog Post
    Published
    Sept. 18, 2018, 1:33 p.m.
    ... by Torsten Wittmann’s lab at UCSF to modulate cellular dynamics with light by modifying EB1. EB1 normally... opens novel ways in manipulating and studying cellular dynamics. The photoinducible control of protein...potential use for a wide range of applications in cellular biology. Van Haren J et al. Nature Cell Biology...the base editing podcast segment The increasing popularity of single base-editors has resulted in the rapid...

  13. CRISPR Challenges: Standardization and Homology Directed Repair

    Type
    Blog Post
    Published
    Feb. 14, 2018, 2:36 p.m.
    ...to learn much more about cellular DNA repair pathways. An emphasis on cellular repair post-CRISPR cleavage...Twitter if you missed our live updates! My most popular tweet from the conference is shown above. The blog..., which alter genomic DNA without the need for cellular HDR.   Having had time to digest my notes, since...

  14. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    Published
    Oct. 29, 2015, 2:30 p.m.
    ...their name? In 1952, Joshua Lederberg set out to clarify the classification of these cytoplasmic inheritance...began to study other extrachromosomal particles, particularly Resistance or R-factors. Like F-factors, R-factors...especially lambda, was the tool of choice for molecular biologists wanting to study bacterial genetics...pSC101 became the first plasmid cloning vector and molecular biology was never the same. Over the next few ...

  15. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    Published
    March 6, 2014, 7:12 p.m.
    ...meetings or calls regularly and don’t cancel, so that you drive progress with regular accountability checks...Mentoring for Scientists Guide.  You can use it to clarify and discuss values. The activity is just the start...Relevant, and Time-bound. It is a good idea to take larger goals (e.g. Figure out what I want to do after ...

  16. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    Published
    June 18, 2019, 1:15 p.m.
    ...often have to choose the best serotype for their particular study, which includes comparing the transduction...samples are available in various serotypes of two popular control AAV encoding GFP. Check out our inventory...variant provides improved detection for tracking large populations of neurons in wide-field imaging (Dana...on availability date or to be notified when a particular catalog item is available for order please email...

  17. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    Published
    May 21, 2019, 12:10 p.m.
    ...are responsible for the degradation of over 600 cellular components ultimately leading to cell fragmentation... scientists need a protease reporter that has a large dynamic range and high brightness. The Shu Lab from... pattern was consistent with TUNEL staining, a popular method to observe apoptotic cells. Lastly, the ...FlipGFP is a novel fluorogenic reporter that has a large dynamic range and high brightness. This reporter...

  18. Addgene Depositors Get More Citations

    Type
    Blog Post
    Published
    April 20, 2017, 2:30 p.m.
    ...outlier, this story had us wondering: is there a larger trend here? Do papers associated with Addgene deposits...deposits are more highly cited, we went to one of the largest bibliographic databases available, Web of Science...Biotechnology & Applied Microbiology, Biochemistry & Molecular Biology, and Biochemical Research Methods. We ...at the MIT Sloan School of Management. She is particularly interested in exploring how innovative tools...

  19. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ...levels for 6 days - after just 15 minutes of FUS.  Similar constructs to the one above can be made for expressing...But why might you need a second GFP antibody?  Similarities:  Generated against full length GFP and converted...the group rationally designed GFP variants with similar capabilities. If you’re looking for a super stable...hfYFP retains folding and fluorescence in high molarity GdnHCl, at high temperatures, and in the presence...

  20. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    Published
    July 17, 2018, 12:23 p.m.
    ...drastically alter your strategy. I realized that similar types of thinking go into developing gene therapy...PLASMID is a game where you need to apply basic molecular biology, cell biology, microbiology, genetic engineering... He is also founder and consultant at FRESCI particularly interested in advanced in vitro models technology...Resource Center Resources on Addgene.org Find molecular biology educational resources Find protocol videos...
Showing: 381 - 400 of 811 results