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Showing: 381 - 392 of 392 results
  1. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...open access journals.   Check Out Addgene Videos Over at the Addgene Youtube Channel   After watching ...Communication 2.2 (2014): 2. Additional Resources on the Addgene Blog 6 Tools & Tips: Online Social Networking ...study processes for diagnosing and resolving organizational performance and often have to be able to quickly...Sharing in Research Publications Resources at Addgene.org Check out our Protocol Pages for Video Protocols...
  2. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    Published
    April 13, 2021, 1:15 p.m.
    ...microbiology Resources on Addgene.org: Visit the CRISPR Guide Get CRISPR plasmids from Addgene ...Plasmids for each of these systems are available from Addgene. If you’ve given any of these systems a try, we...acssynbio.0c00073   Additional resources on the Addgene blog: Browse CRISPR articles from our CRISPR topic...on the escape mechanism of genome editing in microorganisms which could be applied to optimization of genome...
  3. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    Published
    Aug. 11, 2016, 2:23 p.m.
    ...standardize your experiments later. Stay tuned to the Addgene blog and be sure to check out our viral vector ... PMCID: PMC330758. Additional Resources on the Addgene Blog Learn All about Adenovirus Associated Virus...PMID: 12036457. 6. Davis, Howard E., Jeffrey R. Morgan, and Martin L. Yarmush. "Polybrene increases retrovirus...Lentiviral Vectors Additional Resources on the Addgene.org Check out Our Viral Vector Guide Pages Find ...
  4. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ...in setting up a FRET-based assay or screen (the Addgene blog has an excellent primer on FRET and how to...presented (Fig. 1) which can be carried out using Addgene plasmids. His-tag labeling reagents You may already...Gradia Lab has deposited a gateway vector with Addgene, where you can insert your protein of interest,...PubMed Central PMCID: PMC4380784. Resources on the Addgene Blog Get Tips for Using FRET in Your Experiments...unaffected by intervening proteins, lipid bilayers, organelles or other cellular impediments. FRET has been ...Your Fluorescent Protein Basics Resources on Addgene.org Find Plasmids for FRET Browse Empty Plasmids...
  5. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ...pre-existing toolbox for adenoviral vectors in Addgene’s repository is smaller than for other vector types...1566523213666131125095046. PMID: 24279313. More resources on the Addgene blog Viral Vectors 101: Viral Applications Adenoviral...new adenoviral vectors. This post was written by Addgenie Brian O'Neill.  References and resources References....73.8.6930-6936.1999. PMID: 10400791. Kafri, T., Morgan, D., Krahl, T., Sarvetnick, N., Sherman, L., & ...
  6. Plasmids 101: Origin of Replication

    Type
    Blog Post
    Published
    Nov. 11, 2020, 2:23 p.m.
    ...features of a plasmid. So, how do you choose? Addgene Senior Scientist Marcy Patrick says researchers... Kendall Morgan...
  7. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ...mammalian brain (Wickersham et al., 2007; plasmids at Addgene). AAV vectors: A platform for delivering neuronal...their capsid proteins. For a brief overview see Addgene’s AAV guide. AAV particles are relatively small ... production, purification and titration on the Addgene website).  Tissue Optimal Serotype CNS AAV1...Serotypes and their target tissues (adapted from Addgene's AAV guide)  Commonly used serotypes include AAV1...learning and memory (Kohara et al., 2014; plasmids at Addgene). The Gradinaru Lab at the California Institute...the brain based on its unique hue (plasmids at Addgene). A large-scale example of neuronal tracing with...other AAV serotype (Tervo et al., 2016; plasmid at Addgene). Its efficiency is comparable to conventional ...
  8. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...posts @Addgene: The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years Addgene’s Top...2011, it was only recently made available through Addgene and we want to highlight it for use in your research...systems have been developed and deposited with Addgene which allow scientists to use CRISPR-Cas technology...determining the structures are now available from Addgene - bfloGFPa1 and bfloGFPc1. Bomati et al., Sci ...further reading about SpyLigase technology, read the Addgene interview with Mark Howarth. Shining a light...ChrimsonR, CsChR, and CoChR have been deposited to Addgene, including lentiviral and AAV expression vectors... of various optogenetics plasmid tools, visit Addgene’s optogenetics guide. More optogenetics tools...
  9. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    Published
    Oct. 2, 2018, 12:56 p.m.
    ... scientists Resources on Addgene.org Learn about open positions at Addgene Check out our career videos...that loves you back. Additional resources on the Addgene blog Check out our transferable skills guide Learn...encourage diversity in all forms? How are their organizational skills? Are they involved with colleagues ...
  10. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ... As you know well if you’ve been following the Addgene blog, it has been repurposed for genome editing...https://doi.org/10.1038/srep32565 Resources on the Addgene Blog CRISPR 101: Mammalian Expression Systems ...0471142905.hg1508s88 Leidy-Davis T, Cheng K, Goodwin LO, Morgan JL, Juan WC, Roca X, Ong ST, Bergstrom DE (2018...Depression Read About Brainbow Resources on Addgene.org Find CRISPR Plasmids for use in Mammals Check...
  11. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    Published
    Nov. 1, 2018, 12:35 p.m.
    ...PMCID: PMC4511399. Additional resources on the Addgene blog Learn more about CcdB, the toxic key to efficient...toxins to claim territory or kill competing microorganisms, but did you know bacteria also produce substances...about knockout/knock-in plasmids Resources on Addgene.org Check out our molecular biology reference Search...
Showing: 381 - 392 of 392 results