We narrowed to 644 results for: rest
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TypeBlog PostPublishedApril 16, 2015, 3:45 p.m....the intent is to make a knockout in your gene of interest, as it is prone to generating indel errors. Indel...bloggers! David Wyatt is a graduate student interested in determining how the structure of broken ends...
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Summer SciComm Series: Cognitive Load
TypeBlog PostPublishedJune 16, 2022, 1:15 p.m....every project, time, attention, and/or length restraints mean you will have to pick and choose what concepts...feedback in a variety of ways, like having them restate or summarize the concepts you’re covering. Don'... -
Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools
TypeBlog PostPublishedApril 23, 2018, 2 p.m....along with an antibody targeting the protein of interest led to rapid, targeted degradation that is dependent...independently control expression of your genes of interest. These transcription factors consist of the GAL4... -
Human Germline Editing Using CRISPR
TypeBlog PostPublishedAug. 10, 2017, 2:19 p.m....s start with the paper itself: Ma et al. were interested in correcting an autosomal dominant mutation ... vs repair from the maternal MYBPC3 allele. Interestingly, they found that the embryos underwent repair... -
Lighting Up Cell Signaling with Photoswitchable Kinases
TypeBlog PostPublishedJuly 25, 2017, 1:20 p.m....growth factors or serum to stimulate a pathway of interest, but there can be a lot of static since other ...inserting pdDronpa domains into loops of a protein of interest. If you plan to try this, it’s important to check... -
Choosing the B(right)est Fluorescent Protein: Aggregation Tendency
TypeBlog PostPublishedJune 15, 2017, 2:30 p.m....assay with your chosen FP in your cell type of interest before starting your experiments. Selecting a...this fluorescent protein to tag your protein of interest will be problem-free. In figure 1 we provide an... -
Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics
TypeBlog PostPublishedMarch 3, 2020, 2:15 p.m.... allows synthetic biologists to use Type IIS restriction sites to easily assemble multiple DNA fragments...dimerize, bringing each terminus of Cre together to restore Cre activity. As soon as red light or PCB is no... -
Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins
TypeBlog PostPublishedMarch 19, 2019, 1:31 p.m.... approach to simultaneously clone two genes-of-interest (GOI) into two independent expression cassettes...pUC57-L3L2) that offer the advantage of using restriction digest cloning to introduce the GOIs into the... -
The Breast Cancer Microenvironment: A Tumor’s Backstage Team
TypeBlog PostPublishedJan. 31, 2019, 1:32 p.m....considered "natural tumor suppressors." A growing interest in the cancer microenvironment Ever since Stephen...Viva" in Aveiro, Portugal. She is particularly interested in science journalism and writing about biomedical... -
New Tool for Lineage Tracing: The ClonTracer Library
TypeBlog PostPublishedSept. 22, 2015, 2:30 p.m....When we developed this tool, there were so many interesting questions that we could apply [it to].” Using...Pharmacology at Novartis Oncology and is particularly interested in applying what we have learned about tumor ... -
Hot Plasmids and Viral Preps - January 2021
TypeBlog PostPublishedJan. 5, 2021, 2:15 p.m....scientists can control expression of their genes of interest. While recombinases are powerful gene expression...cells to identify cells exhibiting properties of interest. However, FACS is unable to spatially and temporally... -
CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!
TypeBlog PostPublishedOct. 6, 2020, 1:15 p.m....on a guide RNA complementary to the sequence of interest. Upon binding, CRISPRa systems recruit transcription...having multiple copies of VP64 at each loci of interest, this allows more transcriptional machinery to... -
Meet an Addgenie: Insights into Grad School, Career Paths, and More
TypeBlog PostPublishedJune 25, 2020, 1:15 p.m....take me. I’m also just learning. I have a huge interest in data science,” which she’s taking some classes... hours in the lab, she told me, “I was really interested to learn more when I was introduced to bioinformatics... -
Split Fluorescent Proteins for Studying Protein-Protein Interactions
TypeBlog PostPublishedMay 3, 2022, 1:15 p.m..... One popular approach is to fuse a protein of interest to each part of a split fluorescent protein (FP...the behavior of the split FP in your system of interest. Remember that the FP fragments themselves can... -
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation
TypeBlog PostPublishedDec. 14, 2021, 2:15 p.m....considerations: Design your gRNA to overlap the CpG of interest. Having the CpG directly inside the gRNA sequence...but if this is not possible due to gRNA design restrictions, anything within 5 base pairs should work well... -
Rabies and Neuronal Tracing
TypeBlog PostPublishedMay 29, 2018, 1:51 p.m....the G receptor. In neurons, the G receptor is restricted to presynaptic nerve terminals (Lafon 2005). ...applications Use of G deleted RABV in conjunction with restricted TVA and G protein expression is a powerful development... -
Pathways Over Time Plasmids Engage Students in Functional Genomics Research
TypeBlog PostPublishedMay 22, 2018, 1:38 p.m....plasmids are also used to teach students about restriction mapping and western blotting. Students who participate...genetics at Boston College. She is particularly interested in strategies to incorporate research into undergraduate... -
Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools
TypeBlog PostPublishedMay 15, 2018, 12:43 p.m.... CRY2 and CIB1 domains to separate proteins of interest, a researcher can cause them to interact by stimulation...reversible oligomerization when fused to a protein of interest. Fusion protein oligomerization happens within... -
Plasmids 101: Modular Cloning
TypeBlog PostPublishedMarch 12, 2024, 1:15 p.m....from Golden Gate Cloning, in which a type IIS restriction enzyme and a ligase are used to assemble multiple...described with “it’s complicated.” Applying MoClo Interested in using MoClo? You don’t have to start from ... -
Viral Vectors 101: Calcium Sensors
TypeBlog PostPublishedAug. 10, 2023, 1 p.m....noise and ensure you are measuring the events-of-interest. Sensors suitable for ER measurements include:...scientists to focus on specific cell populations of interest. Many of the calcium sensors described are also...