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...and then unfollow those who do not capture your interest during that week.” You can also find Dr. Knoepfler...consistently engage with others about your topic(s) of interest. Hopefully through these links, I’ve: 1) convinced...

...RNA (gRNA) used to target the genomic locus of interest. As these off-target effects can be genome-wide...can allow for targeting of your genomic loci of interest that may not be accessible by wild-type Cas9, ...

...the intent is to make a knockout in your gene of interest, as it is prone to generating indel errors. Indel...bloggers!   David Wyatt is a graduate student interested in determining how the structure of broken ends...

...along with an antibody targeting the protein of interest led to rapid, targeted degradation that is dependent...independently control expression of your genes of interest. These transcription factors consist of the GAL4...

...s start with the paper itself: Ma et al. were interested in correcting an autosomal dominant mutation ... vs repair from the maternal MYBPC3 allele. Interestingly, they found that the embryos underwent repair...

...growth factors or serum to stimulate a pathway of interest, but there can be a lot of static since other ...inserting pdDronpa domains into loops of a protein of interest. If you plan to try this, it’s important to check...

...assay with your chosen FP in your cell type of interest before starting your experiments.  Selecting a...this fluorescent protein to tag your protein of interest will be problem-free. In figure 1 we provide an...

... allows synthetic biologists to use Type IIS restriction sites to easily assemble multiple DNA fragments...dimerize, bringing each terminus of Cre together to restore Cre activity. As soon as red light or PCB is no...

...every project, time, attention, and/or length restraints mean you will have to pick and choose what concepts...feedback in a variety of ways, like having them restate or summarize the concepts you’re covering. Don'...

... approach to simultaneously clone two genes-of-interest (GOI) into two independent expression cassettes...pUC57-L3L2) that offer the advantage of using restriction digest cloning to introduce the GOIs into the...

...considered "natural tumor suppressors." A growing interest in the cancer microenvironment Ever since Stephen...Viva" in Aveiro, Portugal. She is particularly interested in science journalism and writing about biomedical...

...When we developed this tool, there were so many interesting questions that we could apply [it to].” Using...Pharmacology at Novartis Oncology and is particularly interested in applying what we have learned about tumor ...

...the G receptor. In neurons, the G receptor is restricted to presynaptic nerve terminals (Lafon 2005). ...applications Use of G deleted RABV in conjunction with restricted TVA and G protein expression is a powerful development...

...plasmids are also used to teach students about restriction mapping and western blotting. Students who participate...genetics at Boston College. She is particularly interested in strategies to incorporate research into undergraduate...

... CRY2 and CIB1 domains to separate proteins of interest, a researcher can cause them to interact by stimulation...reversible oligomerization when fused to a protein of interest. Fusion protein oligomerization happens within...

...from Golden Gate Cloning, in which a type IIS restriction enzyme and a ligase are used to assemble multiple...described with “it’s complicated.” Applying MoClo Interested in using MoClo? You don’t have to start from ...

...noise and ensure you are measuring the events-of-interest. Sensors suitable for ER measurements include:...scientists to focus on specific cell populations of interest. Many of the calcium sensors described are also...

...on a guide RNA complementary to the sequence of interest. Upon binding, CRISPRa systems recruit transcription...having multiple copies of VP64 at each loci of interest, this allows more transcriptional machinery to...

...take me. I’m also just learning. I have a huge interest in data science,” which she’s taking some classes... hours in the lab, she told me, “I was really interested to learn more when I was introduced to bioinformatics...

.... One popular approach is to fuse a protein of interest to each part of a split fluorescent protein (FP...the behavior of the split FP in your system of interest. Remember that the FP fragments themselves can...