We narrowed to 457 results for: val
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TypeBlog PostPublishedSept. 17, 2015, 2:30 p.m....surgeries and standard molecular neuroscience validation experiments we discussed last week are only half...
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Experimenting in an Open Source lab: from CRISPR to Cats
TypeBlog PostPublishedJuly 23, 2015, 2:30 p.m...., and a metalshop. After two summers in Silicon Valley as a student and then instructor at Singularity... -
Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)
TypeBlog PostPublishedJune 5, 2014, 3:32 p.m....flower that changes color, but the experience is invaluable. This opportunity has given us the chance to ... -
Hot Plasmids: Winter 2025
TypeBlog PostPublishedMarch 11, 2025, 1:15 p.m....of Tau (phospho-Tau). We hope these will become valuable tools for your research! If they do (or if they... -
Common Injection Routes in Mice
TypeBlog PostPublishedJan. 22, 2025, 9:42 p.m....expertise available in your lab or organization, and approvals from the organization overseeing your work. Good... -
Pooled Library Amplifications
TypeBlog PostPublishedMay 30, 2024, 1:30 p.m....amplification protocols attached. Final step: validation Remember, there is no “one-size-fits-all” protocol... -
Twenty Years of Addgene Sharing: CRISPR
TypeBlog PostPublishedApril 18, 2024, 1:15 p.m....Directed Repair has been viewed 219,289 times, while Validating Your Genome Edit has 76,011 views. Has all of... -
Viral Vectors 101: Voltage Indicators
TypeBlog PostPublishedJan. 30, 2024, 2:15 p.m....specific cell types is where viral vectors become invaluable. Viral vectors are mechanisms by which genetic... -
CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments
TypeBlog PostPublishedFeb. 7, 2019, 2:16 p.m....experimental data of its effect on HDR efficiency. These values (ranging from -40 bp to 40 bp) are also converted... -
Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity
TypeBlog PostPublishedOct. 11, 2018, 12:30 p.m....re-ligated pGGA destination plasmids. A final validation of the 5-, 12- or 24-fragment test systems was... -
Transferable Skills: Negotiation
TypeBlog PostPublishedMarch 3, 2022, 2 p.m....provides a scenario where both people get something valuable. Importantly, as the third example shows, the ... -
PhD Applications After COVID
TypeBlog PostPublishedJan. 5, 2023, 2:15 p.m....does COVID-19 impact all of this?” are all very valid questions, and often make an already complicated... -
Plasmids 101: Degron Tags
TypeBlog PostPublishedMarch 14, 2024, 1:15 p.m....conditional degron tag technologies for target validation studies. Nat Commun 13, 5495 (2022). doi:10.1038... -
xCas9: Engineering a CRISPR Variant with PAM Flexibility
TypeBlog PostPublishedMarch 28, 2018, 6:52 p.m.... of phage, increasing stringency and promoting survival of only the broadest-targeting variants. For the... -
Plasmids 101: Knockout/Knock-In Plasmids
TypeBlog PostPublishedDec. 1, 2016, 3:30 p.m....knockout/knock-in systems, these systems remain valuable, especially in cases where CRISPR cannot be used... -
Tips for Using BLAST to Verify Plasmids
TypeBlog PostPublishedMay 29, 2014, 1:29 p.m....frequently on the BLAST website to verify plasmids or validating your new clones, try these tips to make the most... -
CRISPR 101: RNA Editing with Cas13
TypeBlog PostPublishedJuly 31, 2020, 12:30 p.m....will you use these systems in your research? Cary Valley contributed to updating this article. References... -
Capturing a Moment with Marc Zimmer’s “The State of Science”
TypeBlog PostPublishedJuly 30, 2020, 1:15 p.m.... individual topics are not surprising, the real value here is seeing them all in one place. Can we address... -
The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data
TypeBlog PostPublishedJuly 16, 2020, 1:15 p.m....plasmid requests Figure 5: Inferred effects. Values are the inferred percentage of normal activity ... -
Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!
TypeBlog PostPublishedDec. 3, 2019, 11:38 a.m....currently distributing 94 plasmids expressing validated antibodies for neuroscience research (see the ...