We narrowed to 614 results for: CaS;

...fusing catalytically inactivated RNA-targeting Cas13 (dCas13) with the adenine deaminase domain of ADAR2.... as these recent blog posts on prime editing, IgnaviCas9, and Nanoblades), but we hope that this blog ...toolkit for epitope tagging Lentiviral capsid-based Cas9/gRNA ribonucleoprotein delivery Cytosine deaminase... a catalytically impaired Streptococcus pyogenes Cas9. They called this editor Target-ACE (adenine and...Read the paper in bioRxiv Find Target-ACE   CRISPR/Cas9-based toolkit for epitope tagging in neurons Harold... the paper in bioRxiv Find the ORANGE toolkit   Cas9/gRNA ribonucleoprotein delivery by lentiviral capsid-based...capsid-based bionanoparticle Transient expression of CRISPR/Cas9 in cells has advantages like reduced off-target ...

...Pint series. Chapter 4: The SITN Podcast, Sit'N Listen - In their podcast, the SITN team brings in scientists...The Goal of the SITN Podcast - Vini and Amy would like to develop the SITN podcast as a means for listeners...senior.  To learn more about the STIN's podcast, email sitnpodcast@gmail.com    For more info about starting...have to be crazy complicated! Listen to the full podcast for more great science communication tips or listen...of Science in the News, and member of the SITN podcast team. You can follow Vini on twitter @vinidhra....Science in the News, and is a member of the SITN podcast team. You can follow Amy on twitter @aigilson. ...the audio engineer and producer for the Addgene Podcast.     Additional Resources on the Addgene Blog ...

...the usefulness of CPPs must be evaluated on a case-by-case basis. While setting up their CPP system to ...would be compatible with CRISPR/Cas9 genome editing. Although CRISPR/Cas9 is all the rage, they had another... method and its potential applications to CRISPR/Cas9 genome editing.   Protein-only transduction? Niels...expression. Image courtesy of Diego D'Astolfo. CRISPR/Cas9: a potential application of iTOP Having tested iTOP...expression has a long-lasting effect, such as Cre- or Cas9-mediated recombination. Both primary hESCs and immortalized...cells treated with iTOP efficiently took up both Cas9 protein and a gRNA (yes, iTOP can also be used for... indicating that iTOP may be valuable for CRISPR/Cas9 stem cell editing. The small molecule NSDB...

...To overcome this obstacle, a positive selection cassette can be incorporated, and used during the differentiation...the development of site-specific nucleases (CRISPR/Cas9, TALENs, and ZFNs). However, several challenges ...piggyBac™ transposon system can be combined with CRISPR/Cas9 to efficiently perform genetic manipulations in ...along with a site-specific nuclease (such as CRISPR/Cas9) to create a double-stranded DNA break. After DNA..., but defective for integration [4], the donor cassette is removed leaving behind no accessory transposon...recombination and Footprint-Free™ removal of the selection cassette by excision-only piggyBac™. Image courtesy of ...recombinase technology (Cre/loxP) to remove the donor cassette. While this method can be effective, a loxP site...

...fluorescent protein to various Cas9 variants, SpCas9, NmCas9, and St1Cas9. These fusions localized to specific...The catalytically dead Cas9 protein (dCas9) is well known for its ability to bind DNA targets without...inhibition, among others. Over the past few years, dCas9 has also become a robust visualization tool to study...University of Massachusetts Medical School developed a dCas9-based multicolor labeling system to visualize chromosomal... al. “Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow.” Nature...

...Silvana Konermann, the first generation of CRISPR-Cas9 plasmids were not designed with gene activation ...question for her then was this: Can you engineer CRISPR-Cas9 activators that work well enough on any gene that...describe just such a system, which they call CRISPR/Cas9 Synergistic Activation Mediator or SAM. SAM is an... three components: 1. A nucleolytically inactive Cas9-VP64 fusion; 2. A sgRNA incorporating two MS2 RNA...system, Konermann explained, was new knowledge about Cas9’s crystal structure. “I thought maybe we could use...The activator (CRISPRa) sgRNA library uses the sunCas9 system and contains 10 sgRNAs for each transcription...levels of control in the system. First of all, the Cas9s can be turned on and off, to reversibly activate...

... kit to CRISPR/Cas9? When would you choose one or the other? Musunuru: The CRISPR/Cas9 system is easier...system for CRISPR/Cas9 (also available from Addgene) only has two plasmids. In many cases, CRISPR may be ...makes possible, and how it compares to his CRISPR/Cas9 system. Addgene: For people unfamiliar with this...two ends and put them back together, there are occasional errors. That can be advantageous if you are trying...genes that have been implicated in disease or, in cases where particular variants or mutations have been...more efficient. You might often start with CRISPR/Cas9, but TALENs do have more flexibility in terms of...to date suggest TALENs may be cleaner than CRISPR/Cas9. Reference: Quirong Ding et al. "A TALEN genome-editing...

...CRISPR/Cas9-based systems have been developed for generating knockin mice but because CRISPR/Cas9 engineering... of a stop cassette upstream of the target gene in the targeting vector. The stop cassette is floxed, ... arms that flank the gene of interest. In either case, a positive selection marker such as an antibiotic...recombination for precise gene insertion. However, CRISPR/Cas9 systems are more useful for generating knockout ...to engineer knockout mice. ZFN, TALEN, and CRISPR/Cas9 systems can all be targeted to specific regions ...introduce multiple base pair deletions, but CRISPR/Cas9 can also introduce point mutations. While using ...so Cre-mediated recombination deletes the stop cassette, which permits expression of the target gene. ...

...Institute to provide lentiviruses for delivery of SpCas9 and Cas9 nickase for genome editing. You can also enhance...facilitated their fast adoption. However, in many cases the plasmids themselves are only the starting point...Like all things in science, getting a high titer Cas9 lentivirus prep takes a bit of time and effort that... makes it unuseable. With the large size of the SpCas9 nuclease (~4.2 kb) this is a particularly prominent...expression of your gene of interest with a variety of Cas9 activators available in lentiviral format. Finally... pathways. Find Validated gRNAs to use with your Cas9 Virus Pooled libraries now available in lentiviral...be used to infect a cell line already expressing Cas9. As John Doench (Broad Institute and member of the...

...? Check out Addgene's Lentiviral dCas9 SAM vector and Piggybac dCas9 SAM vector! On/off control all the...out the gene for your protein of interest. CRISPR-Cas9 and a custom gRNA is all you need to generate a ...interference (CRISPRi) targets a catalytically dead Cas9 fused to a transcriptional repressor to your gene...repression of protein expression. Addgene has a dCas9 KRAB plasmid for transient knockouts!  Turn it on...) are transcriptional activators fused to a dead Cas9. The system is led, via a custom gRNA, to your gene...protein levels. Looking for a DD? Check out our DD-Cas9 vector! The price of tunable control These systems...Plasmid 101: Repressible Promoters CRISPR 101: Which Cas9 Do I Choose for my Experiment Additional resources...