... apart sources of information they don’t like, looking for reasons to dismiss them. There’s evidence to...Zealand’s multiple Covid-19 lockdowns, I held mask-making workshops in my community. I brought along printed... to better meet the needs of the people we are talking to. Emma Harcourt is a PhD student in the Department...

...Cas9, and the tracrRNA which acts as a scaffold linking the crRNA to Cas9 and facilitates processing of...different components of CRISPR, It's time to start thinking about how you can used them in your experiments...experiments. Use the following flow chart to get you thinking about the types of experiments you're planning on...

...idea to take some time to confirm that you are working with the correct construct, and verify that the...Consult the manufacturer’s manual for the optimal working conditions for each enzyme. Watch out for methylation...run control uncut DNA to ensure your enzymes are working. When uncut plasmid DNA is isolated and run on ...

...— by directly typing terms in a search bar, by ticking some boxes, by selecting a suggestion — how should...resources. Filters We identified the most useful kinds of information per resource category to expose as...our search indices in order to support different kinds of matches: exact words, partial words, phrases,...

...great? It produces a ~10 fold higher titer virus, making it ideal for CRISPR pooled library screening experiments...V2.0 was made available through Addgene in 2015, making this the most recently available plasmid in the... thank all of our depositors and requestors for making their reagents available and for supporting scientific...

...reference protein) under identical conditions, a ranking of the practical brightness can be made (Goedhart...repeating this with another fluorescent protein, a ranking of practical brightness can be made. This strategy...their practical brightness in a system closely mimicking the future application. In figure 2 we provide...

...high-throughput manner and therefore push us closer to making cancer precision medicine a reality. Challenges...Since time is of the essence in clinical settings, looking ahead, we’re starting to build saturating libraries... Institute of Harvard and MIT and is focused on making precision functional genomics a reality for future...

...These enzymes also do not require a protospacer flanking sequence, so you can target virtually any RNA ...Cas13d enzymes have a median length of ~930aa - making them 17-26% smaller than other Cas13s and a whopping...and we at Addgene appreciate our depositors for making their tools available to the broader community....

...think. So, if you’ve ever wondered what goes into ‘making virus’ or what gag and pol stand for, we demystify...recombinant adenovirus is missing the endogenous E1 gene, making it replication deficient. This is typically supplied...discussed are compiled for reference below. Happy virus making!  References and Resources Kulanayake, S., Tikoo...

...can begin to understand how the brain works. It’s kind of like “the head bone’s connected to the neck bone... to the rest of the body. Therefore, by simply looking at how neurons are physically connected we can ...efficiently deliver genetic information or tools, making them useful tracers, but finding a virus that works...

...question that didn’t get answered live - but he kindly took some time to address all your unanswered questions...astrocyte specificity and would like to get it working in microglia as well. A:  We are not aware of any... tail vein injections but would like to avoid "leaking" into the brain, would you recommend using the ...

..., you may be curious about all of the different kinds of antibodies that are available. One common type...References Bradbury ARM, Trinklein ND, Thie H, Wilkinson IC, Tandon AK, Anderson S, Bladen CL, Jones B,...TA, Humphries MJ (2016) Ligand-induced Epitope Masking. Journal of Biological Chemistry 291:20993–21007...

... it’s working. You can only do that screen with a system you are really confident about working for most...made standard protocols available and they are working to further optimize those now. They are also developing...

...better fuels, feeds, foods, and fibers. And because working with plants can be challenging, there are a lot...construct our color changing flower we are carefully making our way through the IP landscape, using plant parts...novel pieces where applicable. We will also be working with open source, transformation technologies like...

... and devoted 100% of his energy and effort into making the startup self-sustainable. Within a year from...all safety nets.” Raising seed money. “Before seeking funding, ask yourself if you really want to do ...Co-founder and formerly VP Operations of Sample6) for taking the time to speak with us about the biotech startup...

...this point, you may be asking yourself, what type of Fab fragment are we talking about here? Many of the...fragment. But this bivalency may cause problems for blocking purposes, since the fragment could bind not only...

...inhibition is spatially and temporally ubiquitous, making them a rather blunt instrument for controlling ...and systematically mutated it. Using a robotic picking approach to select cells with variant characteristics...al, Nat Chem Biol 2018. PubMed PMID:29483642. Making protein-protein interaction studies easier with...

...in some applications (e.g. labeling of cells or tracking promotor activity), but it is problematic in applications...for individual cells, and the number of 'normal' looking cells can be counted. This assay will provide information...on the dimerization tendency in living cells. Strikingly, the OSER assay reveals that tagRFP does not ...

...someone speaks about an affinity reagent, they’re talking about a standard antibody: An ~150kD protein with...nanobodies, are derived from a camelid antibody lacking a light chain, called a heavy chain antibody (Muyldermans...clear: it’s an exciting time to be a scientist looking for that perfect fit!  References and Resources...

...between them, could affect the properties or trafficking of the fusion protein (Kimple et al., 2013). ...opsin-expressing cells). For other things to keep in mind when making fluorescent protein fusions, check out Joachim ...Fluorescent Proteins 101: GFP Fusion Proteins – Making the Right Connection.     Thank you to our guest...