We narrowed to 798 results for: KIN

...475nm Lyn-tag (derived from the tyrosine-protein kinase Lyn) Membranes 3 mCherry 587nm/ 610 nm human ...Anderson JC, Dueber JE, Leguia M, Wu GC, Goler JA, Arkin AP, et al. BglBricks: A flexible standard for biological...

...emerging family of sequencing techniques function as a kind of “GPS for the genome” to compute coordinates for...coffee shops near your house, excluding Starbucks. Taking a nerdy perspective, you might denote your search...

... insert-specific sequence. This is a “yes or no” kind of test, with a positive clone amplifying a product...this type of primer pair anneals to a sequence flanking the insert and one primer anneals to the insert...

...and public health and medical organizations are working to help educate the public about proper antibiotic...observe any mutations at the target site. In a mouse skin colonization model, phagemid treatment decreased...

...library screen only to find the system itself is not working because your reporter protein doesn’t function ...Rajagopala S.V., Stellberger T., Uetz P.  Exhaustive Benchmarking of the yeast two-hybrid system. Nature Methods...

...and develop an area of expertise. Whether you’re seeking a full-time job or want to be an independent grant...a mentor, find your people Find someone who is working or has worked as a Grant Professional to mentor...

...optogeneic actuators and sensors at Addgene. Cell marking/selection: Expression constructs like plasmids ...Because of its stability, GFP can be used in lineage tracking capacities in cell fate studies. It can also be...

...many questions from scientists all over the world looking for some additional information or clarification...functional before investing time and resources into making a stable cell line. When expressing a viral construct...

...The assay only uses one antigen binding antibody, making it less specific than other methods. In addition...positive control will confirm that the procedure is working and should always be included.  For a negative...

...high fidelity during action potentials of fast-spiking neurons. The same A-to-D mutation in the related...useful materials for research into Alzheimer’s, Parkinson’s, ALS, and Huntington’s Disease, including plasmids...

...independently identified LY6A as a receptor for PHP.B by looking for a unique sequence of DNA that was present in...galactose and the AAV receptor (AAVR). Cell lines lacking galactose were still transduced by PHP.B but only...

...clear plans to connect Genotype to Phenotype thus making progress on the NSF “G to P grand challenge.” Creating...like people. This is actually rare in the animal kingdom. Gasterosteus aculeates (Dan Bolnick) A paper on...

...the yeast extracellular surface) which enables tracking of protease activity with a fluorescent protein...that the best motif was from the Lck gene. For marking microtubules of mammalian cells, the author found...

...tools for cellular targeting. Lately, they’ve been working on two tools that expand and exploit CRISPR, both...Jonathan and experts in CRISPR evolution, were also working on the project. Using computational observations...

... Specific High Sensitivity Enzymatic Reporter UnLOCKING (SHERLOCK and SHERLOCKv2) (Gootenberg et al., ... Specific High Sensitivity Enzymatic Reporter UnLOCKING Cas13 (C2c2) was first identified in 2016 by the...

...research vision uninterrupted and then you will begin working through your aims over the next 45 minutes. The...salient details. Make the most of your limited speaking time. Beyond the information you seek to convey...

... used as a test to see how well treatments are working to fight the cancer.  Beyond early modifications...Asbestos Disease Awareness Organization (ADAO) are working toward such a ban, and the president of the organization...

...lentivirus to be time consuming, difficult, and lacking in reproducibility. Read on for some tips for getting...overly confluent or too old. Ensuring that you are working with healthy, happy cells is one of the easiest...

...positive selection and a p15A origin of replication, making it incompatible with other p15A plasmids. pLys ...of Replication The Promoter Region – Let's Go! Looking for More Plasmids 101 Articles?  Find them all ...

...biologist you would say. The student was of course talking about the now well-known FRET, aka Fluorescence...and the extinction coefficient of the acceptor (linking the quantity of absorbed light, at a given wavelength...

..., termed BASU. BASU exhibits 1,000 fold faster kinetics and increases the signal to noise ratio 30-fold...more recently cell-cell interactions. If you’re looking to identify interactions with your favorite protein...

...thoughts on research statements. Snapp, who is always looking for ways to improve his eBook, adds: "If you have... fit into a department and bring in grants. What kind of colleague will you be? Do you understand what...

... are equal. Created with BioRender.com. This kind of normalization is a nearly ubiquitous tool, but...controls So how can you address these errors without making incorrect assumptions? The answer depends on the...

... so, the WHO recommends one strategy: testing, tracking, and social distancing. With countries like South...relies on its activity on the target regions thus making it an affinity-based method. Due to the mismatch...

... signal is boosted and enables single molecule tracking in living cells. The HA frankenbody is an HA tag...also be fused to cellular toxins, radioisotopes, cytokines, and enzymes for cancer, autoimmune, and/or inflammatory...

...fed a diet of primarily egg whites would develop skin lesions and other terrible health issues. This disease...PMC3223083. 6. McInnes, J. L., Forster, A. C., Skingle, D. C., & Symons, R. H. “Preparation and uses of...

...optimal promoter for your gene of interest, painstakingly cloned into the perfect empty backbone, made...Promoter Region – Let's Go! Mammalian Vectors Looking for More Plasmids 101 Articles?  Find them all ...

...cell types and are useful if you're going to be working in several different systems or want to transduce...j.omtn.2017.03.009 Watakabe, A., Ohtsuka, M., Kinoshita, M., Takaji, M., Isa, K., Mizukami, H., Ozawa,...

...been observed to occur in many different kinds of plasmids, taking many forms (Levene, 2009; Higgins & Vologodskii...

... MQ1.) The Q147L mutation improves methylation kinetics such that cytosine methylation occurs within 24...Sander JD, Reyon D, Bernstein BE, Costello JF, Wilkinson MF, Joung JK (2013) Targeted DNA demethylation...