We narrowed to 888 results for: URE

...thousands of investigators studying them, how do we ensure that the research community has access to all of...24651010. 3. Ledford, Heidi. "The ras renaissance." Nature 520.7547 (2015): 278-280. PubMed PMID: 25877186...

...much more predictable than NHEJ. As seen in the figure above, short (5-25 bp) regions of homology flanking...knocking the GFP-Puro cassette into the locus (see figure below). The double-MMEJ strategy looks very similar...

... a little assistance at some point during the procedure. Be an active partner Everyone’s learning style...printed protocol, or maybe you would like to take pictures/videos of a machine set up while your mentor is...

... two sgRNAs isn’t feasible for your experiment, ensure your sgRNA sequence has little homology to other...mediates efficient homology-directed repair and temperature-controlled genome editing. Nat. Commun. 2017 ...

...process that will avoid such miscommunications in the future. Assumptions: Making incorrect assumptions can...Resolving conflicts is great, but avoiding unnecessary future conflicts while giving people the tools to resolve...

...Adeno-associated virus (AAV) has many features which make it a great viral vector, but its packaging ...in mice. Expression from the hybrid vector, as measured by β-galactosidase activity, was 81% of that from...

...-based applications in your experiment...but not sure where to start or what your options are? Addgene's...

...101 covers everything from plasmids parts and nomenclature to popular cloning techniques and troubleshooting...

...milestones Addgene is poised to reach in the near future! We’re almost at:  100,000 different plasmids ...

...parts of every quick tip video on our TikTok! Be sure to subscribe to our YouTube channel for quick tips...

...equipment to learning about how to use a tissue culture hood and other lab equipment, we’re here for you...

...to our collections pages, to comb through the literature for potential new deposits, and to meet with ...

...photos and slideshows shared some of the moments we captured over the years, including an album of Addgenie...

...you inspired by our 2017 #Deckthelab winners? Be sure to enter our 2018 #Deckthelab contest when the next...

...certain export control exceptions were made by the US Bureau of Industry and Security, pursuant to the international...

...Resources from the Addgene Blog Visit our CRISPR featured topic page Check out our Hot Plasmids Podcast ...

...your favorite one. If you have any suggestions for future GIFs, feel free to let us know on Twitter @addgene...

...export control licenses is available through the US Bureau of Industry and Security. If you have any questions...

...Microbiology Microbe meeting here in Boston next week - be sure to come by booth #851 and say hello! Just in time...

... her incredibly important role in solving the structure of DNA. 2. The Immortal Rap of Henrietta Lacks...

...scientists from a variety of different fields. Exposure - With over 100,000 views each month, the Addgene...

...some behind-the-scenes peaks of Addgene’s people, culture, and workplace. Rumor has it that a few Addgenies...

... its own (highly Tweetable!) audio clip. In the future, you’ll be able to access these snippets here or...

...community is your negative data. We want to make sure people are selecting AAVs that will work for their...

... and volume, and submit your request.    Figure 1: The AAV Packaged on Request banner.   We...

...it's an inhibitory opsin!) and due to its bistable nature, it enables inhibition of synapses distant to the...

...transfer plasmids and five different serotypes. Not sure what to request? Addgene's Data Hub can help, with...

...improved site! Here are some of the exciting new features on the blog that will give you a better experience...

...Addgene since our inception in 2004. In the near future, we hope to eliminate much of white space on this...

...contrasts with polyclonal antibodies, which are mixtures of monoclonal antibodies obtained from serum.  ...

...researcher, or technology you would like us to feature in the coming year. Finally, we are always looking...

...t have to keep buying more freezers. I am pretty sure that the world will go on without my whole-cell ...

...individual plasmids as transformed bacteria in stab culture format. Our lab team creates stabs for each plasmid...

...use of optogenetics tools to inhibit epileptic seizures in a mouse model. I sat in on a symposium discussing...

...good habits that I wanted to pass on to you to make sure you are getting the most out of the data you get...

...do you hope Addgene will accomplish in the near future? Right now it’s a matter of maneuvering through...

... to launch an e-magazine this year which would feature articles, posts, and sci-art that would ultimately...

... terms of mentoring style and expectations. Make sure you will enjoy being in that lab and interacting...

...and tissue engineering. How the multidisciplinary nature of the Center for Regenerative Medicine accelerates...

...people when you can. The colleagues you help will be future advocates and references. Collaborate with another...