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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 705 results for: ANG

Showing: 401 - 420 of 705 results

  1. What's New in CRISPR - September 2019

    Type
    Blog Post
    Published
    Sept. 10, 2019, 12:56 p.m.
    ...transcriptional activation and genome editing Yu Wang's lab developed drug inducible CRISPR/Cas9 systems... Jennifer Tsang...

  2. Who Gives a Tweet? 9 Facts About Scientists on Twitter

    Type
    Blog Post
    Published
    June 26, 2017, 1:31 p.m.
    ...Discipline. Biologists, neuroscientists, and ecologists (orange box) post tweets containing scientific domains ...network of life (green), physical (blue) and social (orange)  scientists largely cluster by discipline, suggesting...

  3. In Living Color: The Skinny on In Vivo Imaging Tools

    Type
    Blog Post
    Published
    March 27, 2014, 5:14 p.m.
    ...proteins that would fall within that near-infrared range, and that’s exactly what iRFPs achieved. Those proteins... proteins that operate within the 750 to 800 nm range, and his team is also looking in the direction of...

  4. We're Updating Our Hold for Publication Policy

    Type
    Blog Post
    Published
    June 24, 2025, 1:15 p.m.
    ...are distributed. Next month, we are making two changes to the policy that will make the process a bit ...updating our Hold for Publication policy with two changes that will help us ensure that plasmids don’t get...

  5. Build Your CRISPR Vocabulary

    Type
    Blog Post
    Published
    July 2, 2024, 1:15 p.m.
    ...tracrRNA pair or an sgRNA. It is often used interchangeably with the term sgRNA. Seed sequence: The 8-10...cheat sheet for that! Resources Further reading Wang, J. Y., Pausch, P., & Doudna, J. A. (2022). Structural...

  7. Sensing Neuronal Dopamine

    Type
    Blog Post
    Published
    July 30, 2019, 12:59 p.m.
    ...relatively slow and is not suited to detect rapid changes in DA concentration. To address this, researchers...29853555. PubMed Central PMCID: PMC6287765. Sun, Fangmiao, et al. "A genetically encoded fluorescent sensor...

  8. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    Published
    Feb. 14, 2019, 2:35 p.m.
    ...are ever recycled, so adopting a few meaningful changes in the lab could make a big difference in our global...Many scientists are already leading the cultural change toward responsibility in our lab’s environmental...

  9. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

    Type
    Blog Post
    Published
    Sept. 12, 2023, 1:15 p.m.
    ...Sometimes DNA sequences need to be “cleaned up” or changed in some way before you can analyze them. These ...Data-optimized Assembly Design (DAD): This tool designs overhang sets for high complexity Golden Gate Reactions...

  11. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    Published
    Dec. 14, 2020, 2:15 p.m.
    ...conformational change in the GPCR, leading to the activation of the Gα subunit. The activated Gα exchanges bound...

  13. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    Published
    June 4, 2020, 1:22 p.m.
    ...contamination.  Extreme pH can denature, break, and even change the DNA sequence. High pH favors the breakage of...for the sugar backbone of the DNA helix or even changes the DNA sequence through a mechanism called depurination...

  15. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    Published
    Dec. 12, 2017, 3:13 p.m.
    ...blogs to keep them current. Here are some of the changes we’ve made: New CRISPR plasmid pages We’ve given...RNA targeting systems, RNA editing does not make changes to the genome, thus providing an avenue for temporary...

  17. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    Published
    June 30, 2015, 2 p.m.
    ...sequences and then testing various combinations of changes. The basic functional version of the Sleeping Beauty...contain two SfiI restriction sites with different overhangs depending upon whether they are on the 3’ or 5...

  18. Viral Vectors 101: Transductions

    Type
    Blog Post
    Published
    April 30, 2024, 1:15 p.m.
    ...virus to deliver nucleic acids? Sounds sort of dangerous at first suggestion! The good news is that viruses... the right safety considerations, they aren't dangerous for controlled lab usage. Using viral transductions...

  19. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    Published
    March 26, 2019, 1:40 p.m.
    ...single-wavelength sensors with intensities that change in response to calcium binding. There are a few... through imaging. Since it’s illuminated by red/orange light, Archon1 can also be used in conjunction ...

  20. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    Published
    Aug. 11, 2020, 1:15 p.m.
    ...which have recognition sites in the ITRs. Regular Sanger sequencing typically fails to sequence through ...and high GC content, but some companies now offer Sanger sequencing conditions which can read through this...
Showing: 401 - 420 of 705 results