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  2. Sensing Neuronal Dopamine

    Type
    Blog Post
    Published
    July 30, 2019, 12:59 p.m.
    ...relatively slow and is not suited to detect rapid changes in DA concentration. To address this, researchers...29853555. PubMed Central PMCID: PMC6287765. Sun, Fangmiao, et al. "A genetically encoded fluorescent sensor...

  4. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    Published
    Dec. 14, 2020, 2:15 p.m.
    ...conformational change in the GPCR, leading to the activation of the Gα subunit. The activated Gα exchanges bound...

  6. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    Published
    Feb. 14, 2019, 2:35 p.m.
    ...are ever recycled, so adopting a few meaningful changes in the lab could make a big difference in our global...Many scientists are already leading the cultural change toward responsibility in our lab’s environmental...

  7. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    Published
    June 30, 2015, 2 p.m.
    ...sequences and then testing various combinations of changes. The basic functional version of the Sleeping Beauty...contain two SfiI restriction sites with different overhangs depending upon whether they are on the 3’ or 5...

  8. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    Published
    Aug. 11, 2020, 1:15 p.m.
    ...which have recognition sites in the ITRs. Regular Sanger sequencing typically fails to sequence through ...and high GC content, but some companies now offer Sanger sequencing conditions which can read through this...

  9. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    Published
    March 26, 2019, 1:40 p.m.
    ...single-wavelength sensors with intensities that change in response to calcium binding. There are a few... through imaging. Since it’s illuminated by red/orange light, Archon1 can also be used in conjunction ...

  11. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    Published
    July 21, 2022, 1:15 p.m.
    ...past and chatting with individuals whose research ranged from wildlife conservation to abortion law to poetry...confluence of science and the world it hopes to change. Most researchers are aware of the importance of...

  12. RUBY-Red Siliques

    Type
    Blog Post
    Published
    March 1, 2022, 2:15 p.m.
    ...site linkages and a terminator. Image from He, Y., Zhang, T., Sun, H. et al. Hortic Res. (2020).   I ...  References and Resources References  He, Y., Zhang, T., Sun, H. et al. A reporter for noninvasively...

  13. Viral Vectors 101: Transductions

    Type
    Blog Post
    Published
    April 30, 2024, 1:15 p.m.
    ...virus to deliver nucleic acids? Sounds sort of dangerous at first suggestion! The good news is that viruses... the right safety considerations, they aren't dangerous for controlled lab usage. Using viral transductions...

  14. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    Published
    Nov. 18, 2016, 3 p.m.
    ...and virology Minigenomes - A Safe Way to Study Dangerous Viruses Contributed by guest blogger Tessa Cressy...and retract over many microns in response to pH changes without the need for ATP or other chemical fuel...

  16. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    Published
    Oct. 19, 2023, 1:15 p.m.
    ... the feature its characteristic T-shape. The arrangement of the B-B’ and C-C’ palindromic sequences determines... ITR sequences?  Next-generation sequencing and Sanger sequencing might fail to correctly read through...

  17. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ...were compared head-to-head for gene expression changes of non-target genes and RNA-seq of a nuclear (MALAT1...CAR–T cells was recently devised by the Yingxiao Wang lab.  The crux of the system is a set of heat-inducible...

  18. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    Published
    Feb. 20, 2018, 2:30 p.m.
    ... The space was modern with clean bold colors of orange and white. The decor was very contemporary and ...hours. It was robust, capable of generating a wide range of insert sizes and also produced high yields of...

  19. The MTA at Addgene

    Type
    Blog Post
    Published
    July 23, 2024, 2:17 p.m.
    ...or agreement that states the terms of use for a tangible research material that is shared between two parties... via an online process, though the process has changed a lot over the last twenty years! This has helped...
Showing: 401 - 420 of 691 results