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Showing: 401 - 420 of 600 results

  1. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    Published
    April 8, 2025, 1:15 p.m.
    ...approach is reliable, but it’s overkill in many cases. Often, you may not need to know the exact quantity...Although this assumption is likely true in many cases, you should validate it for your particular setup...

  2. Transferable Skills Guide: Time Management

    Type
    Blog Post
    Published
    Dec. 5, 2017, 1:52 p.m.
    ...any additional work you might need to do. In most cases, I would recommend the second option for the experiment... gave me time to listen to some of my favorite podcasts. JUST GET STARTED - One Harvard Business Review...

  3. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    Published
    Aug. 23, 2016, 2:30 p.m.
    ...to the occasion Don’t just plan to reuse a prepared talk unless it perfectly fits the occasion.  I speak...

  4. A Control for All Seasons

    Type
    Blog Post
    Published
    Oct. 5, 2023, 1:15 p.m.
    ... will increase the power of the results. In this case, mouse samples from positive (+), negative (-) and...antibodies will be highly specific, but in some cases they may cross-react and bind to more than their...

  5. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    Published
    March 8, 2021, 2:41 p.m.
    ... film or with specific imaging devices. In most cases, the primary antibody, which binds the protein of...inconsistent levels of the loading control. In these cases, the western should be repeated.  Finally, while...

  7. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    Published
    March 7, 2023, 2:15 p.m.
    ...designations all refer to the same test. On some occasions, there is an experimental group being compared... being between control vs. experimental. In this case, save yourself from family-wise error and just perform...

  8. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...to knock-down expression of your target or CRISPR/Cas9 to create mutations that essentially eliminate expression...gene for your protein has been mutated using CRISPR/Cas9. You check the mutation and it causes a large deletion...

  9. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ...antibodies together in the same color panel. In this case, make sure to compare the excitation/emission spectra... so it's better to use a brighter fluorophore in case the CD45 expression turns out to be weak. Table ...

  10. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ...been infected with a microbe, to trigger immune cascades that clear these infections. Anything that generates...immune response and initiates different immune cascades. In the research setting, antibodies of different...

  11. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ... This makes it possible to use a polycistronic cassette that expresses multiple reprogramming factors ...minicircle expressing a 2A peptide polycistronic cassette comprised of OCT4, SOX2, LIN28, NANOG, and a GFP...

  12. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ... double peak even with a single product. In that case, you’ll want to validate your primer/probe through...Higgins, 2000; Ref, Zhu, 2002), though there are cases in which it is an appropriate choice (Panina, 2018...

  13. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...ability to cross the BBB, or the PNS, while in many cases targeting the liver less. Because these systemic...being geographically constrained to the brain. In cases where it’s critical to minimize or eliminate off-target...

  14. Meet Aavery, Blugene's New Virus Friend!

    Type
    Blog Post
    Published
    Feb. 13, 2020, 2:34 p.m.
    ...the video to find out the final four! (Note: For cases where the same name was submitted by more than one...

  15. Addgene's 20th Anniversary Party

    Type
    Blog Post
    Published
    June 11, 2024, 1:15 p.m.
    ...headquarters in Watertown, Massachusetts, for the occasion! Addgenies enjoying the party. There are so ...

  16. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    Published
    May 13, 2019, 12:01 p.m.
    ...knockouts using CRISPR and Wednesday will highlight some Cas9 variants. Next, we’ll look at various CRISPR applications...

  17. 2021 Deck the Lab Winners!

    Type
    Blog Post
    Published
    Jan. 25, 2022, 2:15 p.m.
    ... were absolutely abuzz with anticipation as they cast their ballots for their favorite submission. So ...

  18. Twenty Years of Sharing Science!

    Type
    Blog Post
    Published
    Jan. 8, 2024, 3:25 p.m.
    ...it's hard to summarize, but we wanted to mark the occasion by trying.  When we started in 2004, we were...

  19. The Codon Guide to Synthetic Biology

    Type
    Blog Post
    Published
    Sept. 14, 2023, 1:15 p.m.
    ... blogs, free courses, YouTube channels, books, podcasts, and communities to help people get started in...
Showing: 401 - 420 of 600 results