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Showing: 401 - 420 of 686 results

  1. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ...transfection drawbacks: transient expression and needing to work with a transfectable cell line. 2) They... process its own pre-crRNA arrays eliminates the need to include multiple promoters to drive crRNA expression...

  2. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ...you would be oversized even before considering the need for some manner of polyA. In this scenario, you ... labeling at various doses.  As always, you will need to discuss injection volumes with your IACUC committee...

  3. Hot Plasmids - February 2022

    Type
    Blog Post
    Published
    Feb. 22, 2022, 2:15 p.m.
    ... easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid...popularity of FLAG and HA tags, you may not even need to clone new constructs to label your favorite protein...

  4. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    Published
    March 21, 2023, 1:15 p.m.
    ...like the right approach for your experiment, you’ll need to decide exactly which type of virus to use. Types... viral particles inside the nucleus, without the need for envelope disassembly. Both lenti and retro viruses...

  5. Clear and Accessible Writing

    Type
    Blog Post
    Published
    Feb. 15, 2024, 2:15 p.m.
    ...writing applied to alt text. We also were aware of the need for clear, accessible writing in scientific writing...are blurred after centrifugation and the syringe needle is inserted bevel-up at the interface between the...

  6. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    Published
    Feb. 28, 2023, 2:15 p.m.
    ... price. With this increased complexity comes the need (and/or opportunities) to modify the device for ...components are adapted and combined with creativity and feedback from the community to create state-of-the-art ...

  7. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    Published
    June 2, 2016, 2:30 p.m.
    ...biologists. Almost as soon as I began, I realized that I needed a system to keep track of the files that I was ...take to help you plan your analysis. There is no need to go into a lot of detail – the details will be...

  8. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    Published
    Dec. 10, 2013, 3:13 p.m.
    ...useful in generating the mutant cell lines that one needs to do disease modeling in stem cells. Addgene: Tell...TALEN kit includes 834 plasmids for the pieces you need to assemble. Our system for CRISPR/Cas9 (also available...

  9. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    Published
    Sept. 8, 2020, 2 p.m.
    ...creatine. In order to reach the brain, creatine needs to be transported from the blood and cross the blood...effector functions. It’s an important process that we need to understand so that we could possibly develop ...

  11. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    Published
    Jan. 31, 2019, 1:32 p.m.
    ...is touring. But you know what a touring musician needs? A backstage team. Meet the microenvironment, a ...cancer microenvironment Ever since Stephen Paget’s “seed and soil” theory in 1889, microenvironments have...

  13. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    Published
    Nov. 30, 2021, 2:15 p.m.
    ... and by offering advice on areas where you might need some help. Today in our transferable skills guide...revealed another issue in the process, you might need to go back to the beginning, reassess the situation...

  14. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    Published
    July 14, 2021, 1:15 p.m.
    ...variability. Once an animal has undergone a terminal bleed to acquire all of its serum, there is no more of...antibody composition available. If you’re going to need a large quantity of antibody for your experiment...

  15. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...agreements and electronic signatures to dramatically speed up MTA approvals. In 2013 we were thrilled to receive..., with Addgene’s support, could fill a community need for high-quality service, open data, and increased...

  16. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ...complementary site in the genome where it will proceed to generate a blunt DNA double strand break. Cas9...stranded DNA degradation. Cas3 DNA digestion can proceed up to multiple kilobases, but only in one direction...

  17. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    Published
    Nov. 24, 2015, 3:30 p.m.
    ...experimental manipulations, saving the time and money needed to develop new lines. Using nanobodies to create...experimental questions, but not having the tools needed to do so. Cepko lab researcher Jonathan Tang wanted...

  19. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...the stop codon is excised, and gene expression proceeds. One popular lox-stop-lox plasmid is from Tyler... introduce Cre into cells of interest. What if I need two separate recombination events? One potential...

  20. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ... easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid...of H2B-FT consistently predicted the cell cycle speed across these different cell types. In one example...
Showing: 401 - 420 of 686 results