We narrowed to 514 results for: Emb
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TypeBlog PostPublishedJune 24, 2014, 3:59 p.m....as the most efficient means to disrupt the cell membrane and deliver the substrate; however, secreted luciferase...
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Advice for Scientists Starting a Lab
TypeBlog PostPublishedApril 29, 2014, 1:43 p.m....scientists to their labs. Further Reading & Resources EMBO Lab Leadership Courses HHMI Resources for Early ... -
Why and how to start studying chromatin (with Addgene's help)
TypeBlog PostPublishedJune 12, 2018, 11:40 a.m....happens, there are a lot of scientists who not only remember to think about DNA in this context, but also specifically... -
Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2
TypeBlog PostPublishedMay 26, 2020, 1:15 p.m....specific locus to create a knock-out in human embryonic stem cells. Find GEmCherry2 reporter plasmids... -
Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems
TypeBlog PostPublishedMay 19, 2020, 1:15 p.m...., spike proteins, envelope small proteins, and membrane proteins. Although all viral components are available... -
Improved Plasmid Maps Powered by SnapGene
TypeBlog PostPublishedJune 29, 2017, 1:06 p.m....colleagues, or explain plasmid features to a lab member when you’re not at your computer. Maps, sequences... -
Luminescent Imaging with Nano-lanterns
TypeBlog PostPublishedMay 25, 2017, 2:30 p.m....publication in 2012, the Nagai laboratory has assembled a collection of multicolored nano-lanterns for... -
Multiplex Genome Editing with CRISPR-Cpf1
TypeBlog PostPublishedMay 9, 2017, 2:12 p.m....Disadvantages References Yamamoto Lab Golden Gate Assembly transfection One vector expresses Cas9 and ... -
Screening for Successful Genome Editing with Digital PCR
TypeBlog PostPublishedMarch 30, 2017, 2:30 p.m.... in addition to testing their performance (2). Remember that although digital PCR is a great screening... -
Hot Plasmids - February 2022
TypeBlog PostPublishedFeb. 22, 2022, 2:15 p.m.... yet low-pressure teaching opportunity for lab members or students new to protein expression and purification... -
Sweating the Small Stuff: Details in the Lab
TypeBlog PostPublishedFeb. 8, 2022, 2:15 p.m....side to your right as you use them can help you remember what's been added and what hasn't. Developing ... -
Antibodies 101: Isotypes
TypeBlog PostPublishedOct. 19, 2021, 1:15 p.m....tears, saliva, mucus) where it protects mucosal membranes. This isotype is most commonly a dimer with a ... -
Viral Vectors 101: Viral Applications
TypeBlog PostPublishedApril 13, 2023, 1:15 p.m....-transfect cell types ranging from primary to embryonic stem cells. Viral integration of a construct is... -
The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It
TypeBlog PostPublishedJan. 30, 2020, 2:15 p.m....conducting the experiment (note: registrations can be embargoed while the research is ongoing, but all registries... -
Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity
TypeBlog PostPublishedNov. 5, 2019, 1:28 p.m....enzyme can help determine which enzyme to use. Remember, accessibility, specificity, and sensitivity are... -
CRISPR 101: Non-Homologous End Joining
TypeBlog PostPublishedApril 16, 2015, 3:45 p.m.... works in Dale’s lab. Dale Ramsden is a member of the curriculum in Genetics and Molecular Biology... -
Form Your Own Peer Mentoring Group: A How-To Guide for Scientists
TypeBlog PostPublishedFeb. 18, 2014, 4:22 p.m.... multiple mentees efficiently Allows all group members to lead and learn at the same time Broadens the... -
Choosing the B(right)est Fluorescent Protein: Photostability
TypeBlog PostPublishedJune 8, 2017, 2:30 p.m....in microdroplets in a water/oil emulsion, (ii) embedded in a gel, or (iii) attached to a substrate. These... -
CRISPRainbow and Genome Visualization
TypeBlog PostPublishedFeb. 28, 2017, 3:30 p.m....GFP-dCas9 and the gRNA scaffold to enhance complex assembly, thereby decreasing the amount of background fluorescence... -
Summer SciComm: Preprints
TypeBlog PostPublishedAug. 16, 2022, 1:15 p.m....journal and share it more widely! Not everyone embraces preprints as part of their scientific communication...