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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 738 results for: HA

Showing: 401 - 420 of 738 results

  1. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    Published
    June 15, 2017, 2:30 p.m.
    ...analytical ultracentrifugation yields an affinity and has been used to demonstrate that yellow fluorescent ...structure. Based on these data, we conclude that mKOk has dimerization tendency and is not suitable for protein...

  2. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    Published
    June 1, 2017, 2:30 p.m.
    ...well or cannot be detected in a microscopy setup has zero practical brightness and is of no use. Therefore... practical brightness can be made. This strategy has been used in yeast by Lee et al (2013) and in nematodes...

  3. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    Published
    Aug. 4, 2020, 1:15 p.m.
    ...which is now easily available through Addgene and has already been requested dozens of times in 2 weeks...Jackson Laboratory, a nonprofit research institution, has been able to rapidly propagate this strain, making...

  7. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    Published
    Jan. 28, 2020, 2:35 p.m.
    ...on holistic, community-centric science, our team has continuously engaged with a variety of stakeholders...Undergrad category at the iGEM Competition 2019. He has an active interest in scientific writing and science...

  8. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    Published
    Jan. 16, 2018, 3:20 p.m.
    ...Singer’s lab at Albert Einstein College of Medicine has recently published techniques to follow translation...Rojas! Eugenia Rojas, PhD is a neuroscientist who has always been deeply interested in neurobiology.  She...

  10. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    Published
    July 22, 2025, 1:15 p.m.
    ...mammalian systems. Our luciferase collection page has a detailed list of luciferase options. Table 2: Properties...light of a different color instead. This approach has even been used in biosensors where BRET reports on...

  11. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    Published
    Aug. 6, 2024, 1:15 p.m.
    ...gamma-retroviruses) in cancer and stem cell research has prompted the development of multiple virus packaging...components found within the backbone. For example: pLXSN has the wild type LTR, an MCS for cloning X gene, an ...

  12. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...hundred years ago. Since then, medical technology has changed a lot, but viruses have stuck around. Vaccine...been approved by the FDA, and this mode of delivery has a lot of clinical promise moving forward. What viruses...

  13. All in a Twist: dsRNA

    Type
    Blog Post
    Published
    Feb. 2, 2023, 2:15 p.m.
    ...immune response machinery of the host. This pathway has been clinically exploited for antiviral purposes ...deplete expression of proteins of interest. RNAi has even made it into agriculture as species-specific...

  14. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    Published
    July 21, 2016, 2:30 p.m.
    ... human suffering and death. Doing research right has the potential to save lives and money. A 2014 analysis...physiologic or pathologic processes. Much progress has been made in this area in recent years, and you can...

  15. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    Published
    Sept. 22, 2015, 2:30 p.m.
    ...Vivo Pharmacology group at Novartis Oncology, Bhang has been working on using the ClonTracer library to monitor...allosteric inhibitors at Novartis. The ClonTracer library has been distributed all over the world and was recently...

  17. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ...HDR, NHEJ is active throughout the cell cycle and has a higher capacity for repair, as there is no requirement...ends interfere with that step. Accordingly, NHEJ has a vast toolbox of processing factors, including polymerases...

  19. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    Published
    May 13, 2019, 12:01 p.m.
    ...base editing to pooled library screening. It also has information for planning your CRISPR experiment. ...

  20. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ...CRISPR genome editing has made it easier to create knockout alleles in a variety of species, including...including creating Cre-dependent conditional alleles, has remained a challenge. Enter Easi-CRISPR: a method...left homology arm guanine (i.e. GG); this design has been shown to increase T7 promoter activity. To avoid...
Showing: 401 - 420 of 738 results