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We narrowed to 889 results for: IND

Showing: 401 - 420 of 889 results

  1. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...our Hot Plasmids articles.  Here's what you'll find in this post: Peptide-assisted genome editing for...Adapted from Zhang et al. 2023 with permission.   Find Cas-PAGE plasmids here!   Zhang, Z., et al. (2023...on mStayGold inspired and informed this section.  Find mStayGold plasmids at Addgene here!   Ando, R.,... Goeden, et al. 2023, under CC-BY license.   Find the AAV.CAP-Mac plasmid here!   Chuapoco, M.R.,...from Chen et al (2023) under CC-BY license.   Find AAV1-X1 plasmid here! Chen, X., et al. (2023). Functional...has been widely used in a variety of applications. Find anti-DYKDDDDK recombinant antibodies here!...

  2. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    Published
    June 27, 2017, 1:03 p.m.
    ...using FRET! Read on to find out more about this amazing imaging technique and find further tips for using...or the activity of a protein in a synapse. What kinds of tools enable us to measure these properties, ...neuronal activity (voltage sensors), to name a few.   Find Many FRET-Based Biosensors on Addgene's Biosensor...PMC18018. 2. Miyawaki, Atsushi, et al. "Fluorescent indicators for Ca2+ based on green fluorescent proteins ...Biosensors Additional Resources on Addgene.org Find FRET Pairs Check Out Fluorescent Protein Biosensors...

  3. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ...yourself: is it clear how novel my findings will be and why these findings will be worth knowing? This can...tricky, as most of our research builds on ideas and findings from our own or other groups, but your work must...to know what we are doing and why. I always ask kindly friends and family to read my non-expert paragraph...back at my successful applications, I can't always find something that made them better than my unsuccessful...up an ELISA kit company on whose website you can find some great information on ELISA troubleshooting ...

  4. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ...SSRs bind DNA at target sites to induce site specific recombination events: Cre recombinase binds loxP...the Cre-loxP recombination system can be used to induce site-specific recombination events, and that the...loxP sites, while FLP binds FRT sites. When a DNA sequence is flanked by target sites (floxed) in opposing...PMID: 2286602. PubMed Central PMCID: PMC3406316. Find FLEx plasmids from these Addgene depositors: Ed...

  5. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    Published
    Dec. 10, 2020, 1 p.m.
    ...this kind of animal models. Start using adenoviral vectors with your CRISPR/Cas9 research! To find more...cells, Cas9 machinery is transiently expressed and binds to target DNA. In the final step of targeted gene...region. The CRISPR/Cas9-derived RNA-guided nuclease-induced gene disruption frequencies in the various cell...looking for a broader range of CRISPRs plasmid tools, find more plasmids, CRISPR technology guides, FAQs, and...delivery of RNA-guided CRISPR/Cas9 nuclease complexes induces targeted mutagenesis in a diverse array of human...

  6. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    Published
    Aug. 21, 2018, 12:31 p.m.
    ...traditional restriction cloning. Sequence and Ligation Independent Cloning (SLIC) could also be a great choice, ...non-recombinant vectors from propagating. The ligation-independent methods like Gibson and SLIC are also quite efficient...you some tips for your next project. But keep in mind that there are many other cloning methods - including...don’t be afraid to explore multiple approaches to find out which one works best for you. Happy cloning ...simplified, purification-free, sequence-and ligation-independent PCR cloning method." BMC biotechnology 11.1 (...Addgene.org0 Check out protocols for plasmid cloning Find vector backbones Read our molecular biology reference...

  7. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    Published
    April 4, 2019, 12:53 p.m.
    ...site selection by TnsD, a sequence-specific DNA binding protein. Selection removes donor bacteria and recipient...subtilis to the bacterium of interest following IPTG induction of conjugation genes and bi-parental mating. Following...and colleagues used Mobile-CRISPRi to knockdown individual genes in several bacteria associated with human...measured after 6 and 12 generations with or without induction of CRISPRi knockdown. As expected, bacteria expressing...move Mobile-CRISPRi into your bacteria of interest? Find Mobile-CRISPRi plasmids at Addgene! References...Addgene.org Browse our CRISPR interference plasmids Find bacterial CRISPR plasmids Read our CRISPR guide ...

  8. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type
    Blog Post
    Published
    July 13, 2017, 2:30 p.m.
    ... Is it an evolutionarily conserved gene? Can you find an ortholog in the Drosophila genome? Continue reading...describe how you can make your own mutant flies. Find Drosophila Resources at Addgene The birds and the...to help keep your research moving. Below, you’ll find some great introductory resources. As you dive deeper... deeper into Drosophila research, you’ll find more resources that may be more tailored to your field of.... The Bloomington Drosophila Stock Center at Indiana University is another tremendous resource for the...Drosophila Learn about Cre-Lox Resources on Addgene.org Find Drosophila Plamids Drosophila CRISPR Resources Deposit...

  9. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    Published
    June 25, 2024, 1:15 p.m.
    ... and an extension sequence containing a primer binding site and a reverse transcription template (RTT)...high-throughput libraries My labmates and I wanted to find a way to predict the efficiencies of pegRNA designs...the RTT overhang, the GC content of the primer binding site, and many more. Once we had our datasets with...experiments Prediction models only become useful once they find their way into the hands of users. For this reason...resources References Akhtar, W., De Jong, J., Pindyurin, A. V., Pagie, L., Meuleman, W., De Ridder, J....B. (2021). Impact of chromatin context on Cas9-induced DNA double-strand break repair pathway balance....

  10. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ...whether as part of protein fusions or expressed independently) originally fluoresce green or blue and, when... (green or blue) within a population of timer indicates the age of the pool: the higher the ratio, the...is dependent on the total expression time but independent of protein concentration. Calibration curves ... red/green fluorescence falls within the linear window for the theoretical timer shown in Figure 1B, you...cell structure, etc). FP timers should therefore find use in studies of animal development where events...Your Fluorescent Protein Resources at Addgene.org Find Fluorescent Protein Timers Browse All Fluorescent...

  11. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ...following techniques to increase plasmid yield. Keep in mind that you might need to use multiple techniques for...that a higher volume of lysate passed over the binding matrix could clog the matrix when performing filter...determined for each plasmid/strain combination individually, based on determining the cell density or by...Measuring the optical density of the culture can indicate the number of cells present in the given volume... standard 37℃. This plasmid feature should be indicated in the plasmid information or growth strain instructions...coli Strains Additional Resources on Addgene.org Find Info on How to Quantify Your DNA Learn to Do a Bacterial...

  12. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    Published
    June 12, 2014, 3:30 p.m.
    ... switch to Golden Gate Cloning easier because individual labs will not need to ‘domesticate’ all the basic... they were developed mostly with model plants in mind, Arabidopsis and Nicotiana benthamiana. N. benthamiana...Golden Gate cloning system especially with plants in mind. When it’s ready, I’ll make those available on my... up making 30 constructs. They’ve never done any kind of cloning and they don’t have any prejudice about... active on Twitter. Sometimes it’s a good way to find out what’s happening in the office next to you. ...remarkable number of female scientists whose tweets I find really inspirational. They share experiences, articles...

  13. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    Published
    July 30, 2020, 1:15 p.m.
    ...of genome engineering via CRISPR.  Though the individual topics are not surprising, the real value here...Science,” he describes the now infamous tale of Rosalind Franklin and her essential-but-unlauded role in...quieter, more reserved Franklins and Prashers will find it increasingly difficult to compete (for funding... quite a walk down memory lane for me. A brief window in time “The most obvious future consequence of... but that’s unlikely to be foremost in readers’ minds. Six months ago I would have nodded my head knowingly...book left them wanting to learn more. There are individual paragraphs that may have warranted their own ...

  14. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

    Type
    Blog Post
    Published
    Aug. 27, 2020, 1:15 p.m.
    ...protein (FP) is split into two fragments that individually lack fluorescence. When complementary FP fragments...resources in less genetically tractable organisms lags behind. BiFC can also be used to screen for small-molecule...carry out because it relies on a single BiFC pair. Find BiFC plasmids for your experiment at Addgene! How... the random association between FP fragments independent of PPIs. Overexpression can be assessed by comparing...fluorescence due to the random association between FPs independent of PPIs. Simply compare the fluorescence intensity... introduction to FRET Resources on Addgene.org Find fluorescent protein plasmids and resources Visit...

  15. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    Published
    July 1, 2022, 1:15 p.m.
    ...proficiency.  What kind of content are you sharing?  Sometimes your subject matter will drive the kinds of communications... I want to put to rest a common misconception. I find personal bias often unintentionally plays a big ...My dad, on the other hand, mastered the art of finding how-to videos on YouTube long before he learned...audience already is.  You’ll also want to keep in mind that access and availability to resources (including...embark on a project, do your best to understand what kind of resources - including your time - will be required...

  16. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    Published
    Oct. 10, 2023, 1 p.m.
    ...expressing a protein that directly binds to zeocin, preventing it from binding DNA. Zeocin is an all-in-one ...a way to produce more of the plasmid, isolate individual plasmids, or express a gene contained in a plasmid...member of the bleomycin family of antibiotics, which induce cell death by intercalating into DNA and generating...genotoxic when not 100% inhibited, meaning it will induce mutations in the host/plasmid DNA during selection...the Sh ble gene does not fully suppress Zeocin-induced DNA cleavage in human cells. Mutagenesis, 20(2)...

  17. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    Published
    Sept. 8, 2016, 2:30 p.m.
    ... determines how long and precise we can track individual molecules. Poor photon budgets are a widespread... synthetic fluorophore-contianing “ligand” that binds to the tag. Like most good ideas in biological imaging...proteins (FAPs) – These modified antibody fragments bind and enhance small molecule fluorogens (Figure 1e... encoded tag is required as the small-molecule binding motif targets endogenous protein (Figure 1f). ...when free in solution, but high fluorescence upon binding to its cognate protein. In some cases this property...Request Plasmids from the Michael Davidson Collection Find Fluorescent Protein Kits ...

  18. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    Published
    July 7, 2020, 1:15 p.m.
    ... To create the eGFP reporters the Harris lab individually mutated three codons in eGFP to eliminate fluorescence...capability of the seven human APOBEC3 proteins, finding that APOBEC3A and APOBEC3bctd are the most efficient...using a base-editor (C>T conversion) they could induce the translation of any detectable protein, not ...contained the cytidine base editor exhibited robust induction of GFP fluorescence confirming the efficacy of... universal, the Dow lab successfully used GO to induce the translation of an array of different reporters...Resources on Addgene.org Browse the CRISPR guide Find CRISPR plasmids for your research ...

  19. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    Published
    July 25, 2019, 12:03 p.m.
    ...Plant Plasmids and Resources page Find synthetic biology plant plasmids Find CRISPR plant plasmids ...plant. A common method for silencing genes Virus induced gene silencing (VIGS) is a transient technique ...subcellular localization of your protein of interest. Find all plasmids from the Lee-Parsons lab. Test different...PMC6585625. Unver, Turgay, and Hikmet Budak. "Virus-induced gene silencing, a post transcriptional gene silencing...Addgene blog Read all of our plant biology blog posts Find tips for Arabidopsis transformation Read our Plasmids...

  20. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    Published
    Sept. 16, 2020, 1 p.m.
    ... above showed successful target modification, indicating that AAV is a good delivery vehicle for Cas9....cleavage activity in mammalian cells. SaCas9 produced indels at a similar efficiency to SpCas9, leading the ...tested two genes in mice. For both genes, they saw indel formation and phenotypic changes as early as 1 week...packaged in AAV.   Split AAV approaches  While finding smaller Cas9 orthologs works for some applications... in two pieces and package each piece into an individual AAV. When a cell is transduced by both AAVs, ...Resources on Addgene.org Check out our CRISPR Guide Find CRISPR Plasmids ...
Showing: 401 - 420 of 889 results