Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 618 results for: LIS;

Showing: 401 - 420 of 618 results

  1. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ...valuable tool to study cellular physiology and metabolism under various conditions. LiLac has improved ...capable of local temperature sensing with sub-millisecond resolution and sub-degree temperature differences...

  2. Tips for Getting a Faculty Position

    Type
    Blog Post
    Published
    July 20, 2023, 1:15 p.m.
    ... research is about and what you think you can accomplish. No matter how many times I say this, candidates.... One of my graduate students encouraged me to publish this manual and earn some cash for my expensive...

  3. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ...in eukaryotic cells as an intermediate of heme metabolism (Shemetov et al, 2017). Modern state-of-the-art...protein expression level. State-of-the-art FPs (listed in Table 1) were optimized for efficient binding...

  4. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ...address many areas of microbial research like metabolism. Transport, biofilms, and much more. Because ...Resource, a breeding facility for X. laevis and X. tropicalis, the two most commonly used species of frog. ...

  7. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ...identified through microfluidic-assisted in vitro compartmentalisation for their high binding affinity to TO1-biotin...ingredients in the aptamer soup In addition to these established RNA aptamer fluorophores, more are in development...

  8. March for Science

    Type
    Blog Post
    Published
    April 21, 2017, 2:30 p.m.
    ...amassed nearly 500,000 ‘likes’, while their website lists over 200 partner organizations, and 514 satellite... is required. This is by no means an exhaustive list and every marcher is not necessarily marching for...

  9. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    Published
    Sept. 14, 2016, 2:30 p.m.
    ...used to purify proteins from E. coli, Bacillus subtilis, or Pichia pastoris depending upon your specific...Miller, Corwin, et al. "Adaptation of Enterococcus faecalis to daptomycin reveals an ordered progression to...

  10. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...new empty backbones By Meghan Rego You asked, we listened! One of the most powerful tools in a scientist's...the payload sequence into the genome. The team established that they could reprogram the genomic target ...

  11. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...the right information When using a previously published system in your own work, you’ll need to know (...Bamberg, E., Nagel, G., & Deisseroth, K. (2005). Millisecond-timescale, genetically targeted optical control...

  12. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...levels, most of them will also give you a specific list of the candidate off-target sites. For example, ...given threshold even when you’ve tried everything listed here. For some applications, this is ok, but it...

  13. A Control for All Seasons

    Type
    Blog Post
    Published
    Oct. 5, 2023, 1:15 p.m.
    ....    Flow cytometry In addition to the controls listed above, viability and compensation controls are ...antibody-based experiments, it is by no means an exhaustive list and we encourage you to review the literature for...

  14. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    Published
    Oct. 2, 2018, 12:56 p.m.
    ... Twitter. Be ready for the interview with a long list of written questions for the PI and lab members....too, they may not have had experience writing, publishing, or discussing career opportunities with the ...

  15. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    Published
    July 28, 2016, 2:30 p.m.
    .... The plasmids used to construct these RSMs are listed in the table below and can be requested through...sequences on their 5’ and 3’ ends (for insulation). A list of promoters and terminators used in our GRSM registers...

  16. Finding Your Perfect Job After University

    Type
    Blog Post
    Published
    Jan. 12, 2016, 3:30 p.m.
    ...duration of only three months and made a good job to list on my CV. Three months was a perfect duration for...reason why I chose Australia was because it is an English speaking country with a strong economy and plenty...

  17. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...Originally published Jan 27, 2015 and last updated Dec 17, 2020. This post was contributed by Jordan ...experimentation. In 2014, several manuscripts published in Genetics detailed approaches in which selection...

  18. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    Published
    March 21, 2017, 2:30 p.m.
    ... generations of lentiviral vectors have been established so far, with safety increasing in each generation...methods are broadly used for virus titration: the p24 ELISA dosing method detects the expression of viral capsid...

  19. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    ...for obtaining a material transfer agreement, and list of vendors (such as the vector core at the University...the many primary research papers that have been published at this point.  In brief, some standard opsins...

  20. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...manufacturer and/or in the published literature. Note that the antibody being published doesn’t always indicate...
Showing: 401 - 420 of 618 results