We narrowed to 714 results for: Mak

...If you’re using CRISPR to make a genome edit, how do you know if your CRISPR experiment was successful...different constructs for the reporter to work and to make the desired genome edit:  A donor plasmid that ...

...of their optimally suited temperature ranges and make them grow better at suboptimal ones, which could...within a regular mesophilic host system. This would make the host able to grow at low temperatures suitable...

...terminal repeats (ITRs). ITRs are the elements that make an AAV plasmid an AAV plasmid.  Within the AAV ...and disruptive mutations and truncations that can make vector propagation and sequencing a headache. How...

...and molecular biologists use to tinker with DNA. Making changes to a DNA sequence can help scientists identify... are a few drawbacks with these approaches that makes it challenging for users to achieve targeted diversification...

... experiments and it’s already looking like it’s making a big difference. We can now look at many more ...out there. Addgene: Why was it important to you to make these plasmids available to other scientists quickly...

...testing and a lack of well-characterized genetic tools make it challenging to engineer a specific function in...construct our color changing flower we are carefully making our way through the IP landscape, using plant parts...

...systemically administered in an organism. These features make AAV ideal for human therapies and in vivo laboratory...delivery of genetic cargo to a host cell. In order to make room for a gene of interest, AAVs used in research...

...suppression of expression is faster and easier than making a knockout and is a great way to temporarily reduce...integrated (once or many times) into the genome. Make sure to choose an appropriate promoter to express...

... been talking about Click Chemistry since 2001, making use of robust reactions that work in just about...particularly useful for a kind of cellular computation, making possible multiple logic-gated steps. Beyond the...

...comment online! Want the general public (say, policymakers or community organizations) to use your work...those plasmids alongside a journal article, but it makes the research materials available to the community...

...time to read the book.  Intuitively, this probably makes sense. It’s a lot easier to read a formulaic fiction...happens to us all!) and offer a variety of options to make your content more accessible to your audience.  ...

...short linker peptide. The lack of constant region makes them less immunogenic, but they have lower affinity...produced in bacteria and are generally cheaper to make than antibodies. Minibodies are created by binding...

...developed by engineering the Cas12f scaffold gRNA and making strategic mutations in the DNA binding cavity of...half the size of the Cas9 or Cas12a based systems, making it well suited for AAV packaging. Most importantly...

...opsin-expressing cells). For other things to keep in mind when making fluorescent protein fusions, check out Joachim ...Fluorescent Proteins 101: GFP Fusion Proteins – Making the Right Connection.     Thank you to our guest...

... she couldn’t run enough workshops by herself to make the biggest impact. When she spoke with Teytelman...visualization. Not everyone in the group was able to make it physically to instruct the workshop at the conference...

...promote tumor durability instead of repressing them, making it even harder to cure cancer. To understand how...Witz, Isaac P. "The tumor microenvironment: the making of a paradigm." Cancer Microenvironment 2.1 (2009...

...exposure to autophagy-inducing conditions. Rosella makes it easier to study the mechanisms behind autophagy... shown in Sargsyan et al. It is also possible to make Rosella fusions and thereby monitor autophagy of...

...However, they note that certain improvements could make CRISPRainbow even better. Adding an additional hairpin...activation/repression and genome editing. Ready to make your cells a little more colorful? CRISPRainbow ...

...is no small task. As a scientist, it is useful to make literature searching and reading a regular part ...Keeping up with the literature There are a few ways to make sure you are keeping up with the literature in your...

...When it comes to making the genetic manipulation, there are two main decisions to make: 1. Which modification...