We narrowed to 683 results for: Mos

...degrons, you have options! Below we will review the 4 most common types of degrons and some of the nuances ...degradation. HaloTag is also the degron tag with the most alternative functions — there are antibodies to ...

... protocol. Created with BioRender.com.   The most important factor to consider is your desired library...what transformation efficiency you need to achieve. Most pooled library protocols aim for somewhere between...

...day old human blastocyst [6]. They are isolated mostly from abnormal and/or supernumerary preimplantation... generate specialized cells for their location, mostly to maintain cell numbers or replenish dying cells...

...process of even applying to one of these programs is almost as daunting as the program itself. Picking a mentor...idea to email around three professors per program. Most applications will recommend that you name two professors...

...Pooran's NgAgo Survey The take-home message While most of the respondents have been struggling to achieve...editing in mammalian cells. However, the fact that most of the researchers surveyed here could not achieve...

...common issues follow. It's a matter of concentration Most FPs in nature are prone to dimerization (i.e. EGFP... FPs such as TagRFP. Problem 3 can be solved, in most cases, by using monomeric FPs or FPs from different...

...EMCCD) or complementary metal-oxide semiconductor (CMOS) sensors, it takes only a few milliseconds to acquire...Murray’s clear solution.  Murray's clear (11) was mostly used to study the development of vertebrate embryos...

...be used as a means of detection; they are useful almost anytime you want to express multiple genes in the...genes from a single transcript. The two strategies most widely incorporated into plasmids for research purposes...

...validating your new clones, try these tips to make the most of your time and sequence! You might also enjoy ...GenBank, EMBL, DDBJ, and PDB sequences and may be the most comprehensive for searching. Timesaving Tip #1:...

...fluorescence microscopy, is another option. However, most SRM techniques also require specialized instruments...requires fixed samples. This limitation may be the both most obvious and least flexible one. Tissue expansion...

...’t we use it? This last section was arguably the most important, and it was a little worrisome for me ...basically impossible for textbooks to keep up. Even the most ambitious teachers, who work hard maintaining a ...

...post-translational modifications that bacterial and most yeast cells cannot. Your choice of production system... the amount of protein you need to produce.  The most common yeast strain used for recombinant antibody...

... Types’ I selected ‘Lentiviral’ which eliminates most of the plasmids designed for other uses. I could...inspiration for our updated mascot, it’s also a convenient most requested filter. Applying a second filter, this...

...-contianing “ligand” that binds to the tag. Like most good ideas in biological imaging, the initial breakthrough...proteins makes them the go-to choice for many (if not most) fluorescence microscopy experiments. However, when...

...’s your favorite part about working at Addgene?” Most Addgenies reply with an enthusiastic, “Our company... atmosphere...

...easy for scientists to re-synthesize DNA with the most optimized sequence. Unfortunately, it is not always...compared to others. Image from Hiss et al., 2017. Most commonly, scientists codon optimize genes from eukaryotic...

... the excision of the phage from the bacterial chromosome and the regeneration of attP and attB sites. ...inhibits the growth of CcdB sensitive E. coli strains, most colonies should contain the desired, recombined ...

...every biologist, academic, and researcher. Among the most well-known CRISPR-associated nucleases are Cas9 ...just 408 amino acids long — making it one of the most compact genome editors to date. We have developed...

...this technology, but Oxford Nanopore is one of the most popular options. Nanopore sequencing deviates from...flexibility for discrepancies in backbone sequences. Most theoretical sequences submitted to Addgene have ...

...Antibody here!   Anderson, Z., Zhu, H., Riedel, T., Moshinsky, D., Meijers, R. (2024). Western Blot for Anti-V5...signal and can be used in light-sensitive systems. Most excitingly, assembly of the split luciferase is ...