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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 676 results for: Mos;

Showing: 401 - 420 of 676 results

  2. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...EMCCD) or complementary metal-oxide semiconductor (CMOS) sensors, it takes only a few milliseconds to acquire...Murray’s clear solution.  Murray's clear (11) was mostly used to study the development of vertebrate embryos...

  3. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...be used as a means of detection; they are useful almost anytime you want to express multiple genes in the...genes from a single transcript. The two strategies most widely incorporated into plasmids for research purposes...

  4. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ...validating your new clones, try these tips to make the most of your time and sequence! You might also enjoy ...GenBank, EMBL, DDBJ, and PDB sequences and may be the most comprehensive for searching. Timesaving Tip #1:...

  5. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...fluorescence microscopy, is another option. However, most SRM techniques also require specialized instruments...requires fixed samples. This limitation may be the both most obvious and least flexible one. Tissue expansion...

  6. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...post-translational modifications that bacterial and most yeast cells cannot. Your choice of production system... the amount of protein you need to produce.  The most common yeast strain used for recombinant antibody...

  8. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    Published
    July 9, 2019, 12:52 p.m.
    ...’t we use it? This last section was arguably the most important, and it was a little worrisome for me ...basically impossible for textbooks to keep up. Even the most ambitious teachers, who work hard maintaining a ...

  9. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    Published
    Sept. 8, 2016, 2:30 p.m.
    ...-contianing “ligand” that binds to the tag. Like most good ideas in biological imaging, the initial breakthrough...proteins makes them the go-to choice for many (if not most) fluorescence microscopy experiments. However, when...

  12. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    Published
    July 15, 2025, 1:15 p.m.
    ...this technology, but Oxford Nanopore is one of the most popular options. Nanopore sequencing deviates from...flexibility for discrepancies in backbone sequences. Most theoretical sequences submitted to Addgene have ...

  13. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...Antibody here!   Anderson, Z., Zhu, H., Riedel, T., Moshinsky, D., Meijers, R. (2024). Western Blot for Anti-V5...signal and can be used in light-sensitive systems. Most excitingly, assembly of the split luciferase is ...

  14. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ... the excision of the phage from the bacterial chromosome and the regeneration of attP and attB sites. ...inhibits the growth of CcdB sensitive E. coli strains, most colonies should contain the desired, recombined ...

  16. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ...A similar genetic code is used by most organisms on Earth, but different organisms have different preferences...Nonsense! I should just choose the codon with the most abundant pool of charged tRNAs in my host organism...

  18. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    Published
    May 21, 2024, 1:30 p.m.
    ...field of research], and have excellent [list the most important skills for the position]. I am enthusiastic... does not need to be a literary masterpiece. For most jobs, the writing quality required will be close...

  19. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...easy for scientists to re-synthesize DNA with the most optimized sequence. Unfortunately, it is not always...compared to others. Image from Hiss et al., 2017. Most commonly, scientists codon optimize genes from eukaryotic...

  20. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...several sgRNA’s at the same time, and choose the most effective ones to increase your chances of gene ...the pronuclei were fusing, you may have several mosaic mice. To figure out what alleles are present in...
Showing: 401 - 420 of 676 results