We narrowed to 457 results for: PAC

1. Mouse Modeling, Part 1: Genetically Engineered Mice

   Type

   Blog Post

   Published

   July 11, 2019, 1:26 p.m.

   ...comparing dominant and recessive traits and their impact on diseases or treatments, but are not useful for...

2. A Look at Addgene's QC Process

   Type

   Blog Post

   Published

   May 6, 2025, 1:15 p.m.

   ...discrepancy in the plasmid, and that it is not known to impact the function of the plasmid. If the issue is likely...

3. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

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   Blog Post

   Published

   Nov. 14, 2024, 2:15 p.m.

   ...start site function would also help to reveal the impacts of a lncRNA on nearby genes and its role in gene...

4. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

   Type

   Blog Post

   Published

   July 12, 2021, 1:15 p.m.

   ... are cloned into pLVX vectors (for lentiviral packaging) and contain an IRES-Puro marker for stable cell...

5. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

   Type

   Blog Post

   Published

   April 6, 2021, 12:30 p.m.

   ...number one was to ensure that this changeover had no impact on our ability to provide requesting scientists...

6. CRISPR in the Clinic

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   Blog Post

   Published

   Nov. 2, 2023, 1:15 p.m.

   ...Clinical trials are underway to explore CRISPR’s capacity for both classes of edits in cancer therapy. Blood...

7. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

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   Blog Post

   Published

   Jan. 21, 2015, 1:30 p.m.

   ...options for mutually beneficial solutions. If you package your challenges with your supervisor into learning...

8. xCas9: Engineering a CRISPR Variant with PAM Flexibility

   Type

   Blog Post

   Published

   March 28, 2018, 6:52 p.m.

   ... 64 possible NNN PAM sequences adjacent to a protospacer. If ω-dCas9 could recognize a library PAM, it...

9. Transferable Skills Guide: Managing a Team

   Type

   Blog Post

   Published

   Jan. 30, 2018, 2:42 p.m.

   ...and not valued which will undoubtedly negatively impact your work and your life. It’s up to you to decide...

10. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type

    Blog Post

    Published

    July 2, 2020, 12:40 p.m.

    ...that are distant linearly but close together in space. The chromatin “picture” is then developed by detecting...

11. Plasmids 101: Optimizing Plasmid Yields

    Type

    Blog Post

    Published

    May 26, 2016, 2:30 p.m.

    ...strain Differences between E. coli host strains can impact plasmid yield. Check the bacterial strain to see...

12. Experimenting with New Careers while in Grad School

    Type

    Blog Post

    Published

    Feb. 25, 2016, 3:30 p.m.

    ... once a week to get recharged. The fun change of pace helped break up the negativity and allowed me to...

13. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type

    Blog Post

    Published

    Feb. 7, 2019, 2:16 p.m.

    ...guide sequence to every possible 23bp sequence (protospacer + PAM) in the target region after HDR. If the...

14. Hot Plasmids: Winter 2025

    Type

    Blog Post

    Published

    March 11, 2025, 1:15 p.m.

    ...from a vector backbone that allows lentiviral packaging as a single payload (Figure 4). Figure...

15. Magnetic Control of Proteins: More than a Dream

    Type

    Blog Post

    Published

    Sept. 24, 2024, 1:15 p.m.

    ...-up studies. “We don’t have a vision of passive impact,” he says, noting that his team regularly shares...

16. Genome-wide Screening Using CRISPR

    Type

    Blog Post

    Published

    Aug. 18, 2015, 2:30 p.m.

    ...of the genome and located immediately 5’ to a protospacer adjacent motif (PAM) sequence. Co-delivery of...

17. Which Fluorescent Protein Should I Use?

    Type

    Blog Post

    Published

    May 20, 2014, 2:06 p.m.

    ...wavelength. Thus, FPs are proteins with this unique capacity. Many of these FPs are fluorescent when ectopically...

18. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type

    Blog Post

    Published

    Dec. 12, 2013, 3:35 p.m.

    ...plugging into the probiotic and cellular therapeutic space. If you were to look forward to the future of the...

19. Transferable Skills Guide: Identifying Your Transferable Skills

    Type

    Blog Post

    Published

    Jan. 11, 2018, 3:29 p.m.

    ... is the transferable skills that create a full package and add an extra shine to your work. Knowing your...

20. DNA Purification Without a Kit

    Type

    Blog Post

    Published

    Feb. 11, 2020, 2:15 p.m.

    ...stacked together in series to increase binding capacity. Figure 1: Purify DNA using glass filters...