We narrowed to 458 results for: Spr

1. Antibodies 101: Polyclonal Antibodies

   Type

   Blog Post

   Published

   July 14, 2021, 1:15 p.m.

   ... (2019) Antibody Purification and Storage. Cold Spring Harb Protoc 2019:pdb.top099101. https://doi.org...

2. Click Biology: How to Click and Play to Explore Biology

   Type

   Blog Post

   Published

   Oct. 21, 2025, 1:15 p.m.

   ... CRISPaint Gene Tagging Kit SpyTag/SpyCatcher CRISPR-assisted insertion tagging Kit; integration of ...

3. All in a Twist: dsRNA

   Type

   Blog Post

   Published

   Feb. 2, 2023, 2:15 p.m.

   ...021-00430-1 More resources on the Addgene blog CRISPR 101: RNA Editing with Cas13  RNA Interference ...

4. RNA Extraction Without A Kit

   Type

   Blog Post

   Published

   March 24, 2020, 1:15 p.m.

   ...RNA. Keep a clean work area, which may include spraying your bench down with a product to get rid of RNases...

5. Developing Lab Management Software for Biology

   Type

   Blog Post

   Published

   Aug. 25, 2016, 2:30 p.m.

   ...where you learn biology terms (plasmid, 5-prime, CRISPR, growth strains, vector backbone, etc.) and you...

6. Overcoming the Challenges of Lentiviral Production

   Type

   Blog Post

   Published

   Aug. 7, 2018, 12:31 p.m.

   ...for transfer plasmids with small inserts (such as CRISPR guides) while a single 72h harvest is best for ...

7. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

   Type

   Blog Post

   Published

   June 26, 2018, 1:33 p.m.

   ...testing in the lab will be greatly facilitated by CRISPR reagents and other specific gene sequences available...

8. Adenoviral Vector Production and Troubleshooting

   Type

   Blog Post

   Published

   June 14, 2018, 11:44 a.m.

   ...- a primer for beginners Adenoviral delivery of CRISPR/Cas9 5 tips for troubleshooting viral transductions...

9. Educational Planarian Tools for Teaching Developmental Biology Techniques

   Type

   Blog Post

   Published

   May 24, 2018, 1:20 p.m.

   ...Biology with BioBuilder 3D Models for learning about CRISPR Resources at Addgene.org Find plasmids from the...

10. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type

    Blog Post

    Published

    June 25, 2020, 1:15 p.m.

    ...Perkins presented a poster a few years ago at a CRISPR meeting.Read his Instagram feature here. You...

11. Hot Plasmids February 2024

    Type

    Blog Post

    Published

    Feb. 20, 2024, 2:45 p.m.

    ...cells By Susanna Stroik Even with the advances in CRISPR technology over the past decade, primary cells ...

12. Viral Vectors 101: Calcium Sensors

    Type

    Blog Post

    Published

    Aug. 10, 2023, 1 p.m.

    ...with genetically encoded calcium indicators. Cold Spring Harbor Protocols. 2012 Jun 1;2012(6):pdb-top069609...

13. Early Career Researcher Toolbox: Finding Relevant Papers

    Type

    Blog Post

    Published

    Jan. 9, 2020, 1:45 p.m.

    ...including a bot for biosensor papers, microbe articles, CRISPR papers, and more. Saving papers There isn’t technically...

14. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type

    Blog Post

    Published

    Nov. 7, 2017, 1:59 p.m.

    ...our Viral Vectors Featured Topic Page Learn about CRISPR-AAV vectors Learn about Addgene AAV Quality Control...

15. 5 Tips for Troubleshooting Viral Transductions

    Type

    Blog Post

    Published

    Aug. 11, 2016, 2:23 p.m.

    ...adoptive immunotherapy." Acute Leukemias VIII. Springer Berlin Heidelberg, 2001. 291-306. PubMed PMID:...

16. Tips for Titering Your Lentiviral Preps

    Type

    Blog Post

    Published

    March 15, 2016, 2:30 p.m.

    ... Lentivirus FAQ Learn How Lentivirus is Used in CRISPR Genome-Wide Screening Learn How Lentivirus Has ...

17. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

    Type

    Blog Post

    Published

    Oct. 11, 2018, 12:30 p.m.

    ...illustrates how the volume of the 1 ml outgrowth spread on each transformation plate can be manipulated...

18. Plasmids 101: 5 factors to help you choose the right cloning method

    Type

    Blog Post

    Published

    Aug. 21, 2018, 12:31 p.m.

    .... You’re going to engineer the best thing since CRISPR; you are ready to clone! But...how? Cloning is...

19. Polymerase Chain Reaction Overview and Applications

    Type

    Blog Post

    Published

    Aug. 10, 2021, 1:15 p.m.

    ...identified in the late 1960s during research at hot springs in Yellowstone National Park.  In addition to Taq...

20. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type

    Blog Post

    Published

    Aug. 3, 2023, 1:15 p.m.

    ...t “wash out” the rest of the panel and create spreading error. Conversely, markers with low expression...