We narrowed to 546 results for: TIL;

1. Viral Vectors 101: Viral Applications

   Type

   Blog Post

   Published

   April 13, 2023, 1:15 p.m.

   ...Additional Resources on the Addgene Blog AAV: A Versatile Viral tool for Gene Expression in Mammals Listen...

2. Viral Vectors 101: Gamma-Retroviral Packaging Systems

   Type

   Blog Post

   Published

   Aug. 6, 2024, 1:15 p.m.

   ...using helper-free packaging cell lines This method utilizes a packaging cell line that stably expresses Gag-Pol...

3. Plasmids 101: Inducible Promoters

   Type

   Blog Post

   Published

   Jan. 18, 2018, 2:34 p.m.

   ... inducible tetracycline ON (Tet-On) system, a versatile tool developed for use in prokaryotes and eukaryotes...

4. A Novice's Perspective on Neuroscience - SfN2017

   Type

   Blog Post

   Published

   Dec. 15, 2017, 2:07 p.m.

   ...interested in using AAVs because they provide a highly versatile means of delivering genes to many different cell...

5. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

   Type

   Blog Post

   Published

   March 17, 2020, 1:32 p.m.

   ... of a T7 RNA polymerase and cannot be expressed until the base editor corrects early stop codons within...

6. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

   Type

   Blog Post

   Published

   Jan. 30, 2020, 2:15 p.m.

   ... can be difficult to replicate. Unclear methods Until someone attempts to replicate the results of another...

7. 15 Years of Addgene: The Top 15 Plasmids

   Type

   Blog Post

   Published

   Jan. 8, 2019, 1:54 p.m.

   ... Their long-lasting popularity illustrates the utility these tools have in many different areas of biology...

8. Early Career Researcher Toolbox: Social Media for Scientists

   Type

   Blog Post

   Published

   Dec. 11, 2018, 12:46 p.m.

   ...using them to my professional advantage. It wasn’t until I wrote a blog post about a research paper that ...

9. Plasmids 101: Methylation and Restriction Enzymes

   Type

   Blog Post

   Published

   June 30, 2016, 2:30 p.m.

   ...from the restriction modification systems, yet can still affect whether certain restriction enzymes can effectively...

10. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type

    Blog Post

    Published

    June 2, 2016, 2:30 p.m.

    ...commands entered is useful for beginners who are still learning the basics. However, these notes take up...

11. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

    Type

    Blog Post

    Published

    Feb. 24, 2015, 7:50 p.m.

    ...in my opinion, had not been achieved.” That is, until now. Enter the SAM pooled library In a paper published...

12. How to Make Friends and Meet People at a Scientific Conference

    Type

    Blog Post

    Published

    Aug. 7, 2014, 1:58 p.m.

    ...conference, but start with a more general intro until you know if the person you are talking to is really...

13. 3 Challenges in Plant Synthetic Biology

    Type

    Blog Post

    Published

    July 22, 2014, 5:46 p.m.

    ...over 30 years ago, but plant synthetic biology is still in its infancy. A long timeline from transformation...

14. Summer SciComm: Preprints

    Type

    Blog Post

    Published

    Aug. 16, 2022, 1:15 p.m.

    ...directly alongside the preprint while the work is still in progress or awaiting a final publication in a...

15. Antibodies 101: Affinity Reagents

    Type

    Blog Post

    Published

    April 14, 2022, 1:15 p.m.

    ...trendy as that may sound. Validated antibodies are still an excellent option for many applications, and frankly...

16. Seven Tips for Using LinkedIn as a Scientist

    Type

    Blog Post

    Published

    Oct. 1, 2019, 1:21 p.m.

    ... manager. This is considered intrusive and it’s still connecting with a stranger so is highly discouraged...

17. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type

    Blog Post

    Published

    July 25, 2017, 1:20 p.m.

    ...Raf-MEK-ERK signaling pathway to demonstrate the utility of  photoswitchable kinases in vivo with C. elegans...

18. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type

    Blog Post

    Published

    June 15, 2017, 2:30 p.m.

    ...cells (Shaner et al, 2013; Bindels et al, 2017). Still, the data presented in papers should be used with...

19. Antibodies 101: Fab Fragments

    Type

    Blog Post

    Published

    Sept. 5, 2024, 1:15 p.m.

    ...resulting product is a mini-Y with both Fab regions still attached to one another. Size: ~110 kDa. Fab' - ...

20. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

    Type

    Blog Post

    Published

    April 23, 2018, 2 p.m.

    ...what if you just want to change protein levels? Until recently, no such tool existed for rapid, customizable...