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We narrowed to 863 results for: TOR;

Showing: 401 - 420 of 863 results

  1. Twenty Years of Sharing Science!

    Type
    Blog Post
    Published
    Jan. 8, 2024, 3:25 p.m.
    ...in 2004, we were the plasmid repository. In 2016, we added viral vectors, and in 2022 we added ready-to-use...ready-to-use antibodies. Now, we think of ourselves as a repository for DNA-based materials, including our popular... kits and libraries. This DNA-based materials repository contains 139,868 reagents from 5,821 labs in ...international community, we've partnered with distributors in China, Korea, Japan, India, and Brazil.  ... system for scientists using materials in our repository, from our customer and science support to our...

  2. Addgene’s New Way to Help You Share Science

    Type
    Blog Post
    Published
    Nov. 14, 2013, 4:10 p.m.
    ...amazing stories to share. I spend a lot of time telling scientists about Addgene’s repository, and explaining...student, post-doctoral fellow, lab manager, research technician, or Principal Investigator. How-to guides...for genome editing? Know a professor with a great story to tell? Let us know by emailing us at [email protected] on Twitter and Facebook. We’ll track down the story, get the inside scoop, and keep you informed.   ...

  3. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    Published
    July 9, 2019, 12:52 p.m.
    ...m a father of three, a scientist and science communicator, and I strive to be a volunteer and contribute...scientist. When our elementary school needed a new coordinator for the annual Math & Science Night, I took the...contacting our school district’s science curriculum coordinator with minimal luck, but eventually the 8th grade...for a scientist. I think that establishing this history helped demonstrate that a) I was a real person ...great feedback from teachers and other science communicators.  What can CRISPR do? I spent more time on...which demonstrated to the students that scientific history was very much in the making. One of the exciting... in an exciting, tumultuous time in scientific history. Whatever your reasons, let us know how it goes...

  4. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    .... (2007) ‘Improved Gateway Binary Vectors: High-Performance Vectors for Creation of Fusion Constructs ... J. (2002) Arabidopsis : a laboratory manual. Cold Spring Harbor Laboratory Press. Available at: https...expression system Choosing an appropriate expression vector is the first step in generating transgenic Arabidopsis...from the Tsuyoshi Nakagawa lab are binary gateway vectors and can be used for promoter swapping and generating... ‘A robust family of Golden Gate Agrobacterium vectors for plant synthetic biology’, Frontiers in Plant...

  5. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    Published
    July 16, 2020, 1:15 p.m.
    ...significant holidays. Figure 4: Plasmid request trajectories suggest an exponential decay demand model with... scientists or labs placing orders.  Our model factors the likelihood in our Poisson process into two ... multiplies the likelihood by the corresponding factor 𝜙z if t is during event z. The "events" we considered...each region using stochastic gradient descent in Pytorch, which gave us the maximum likelihood estimates...chemistry and chemical biology.       David R. Liu is Director of the Merkin Institute and Vice-Chair of the ...University; and Howard Hughes Medical Institute Investigator. Liu’s research integrates chemistry and evolution...four examples of technologies pioneered in his laboratory. Learn more here. Additional resources on the...

  6. The Developmental Studies Hybridoma Bank: Over 25 Years of Antibody Sharing

    Type
    Blog Post
    Published
    March 10, 2015, 2:25 p.m.
    ... Bank (DSHB), directed by David R. Soll, is a repository for monoclonal antibodies, distributing over ...antibodies to be used as a powerful reagent in the laboratory.   The DSHB focuses on monoclonal antibodies,...include those directed against human transcription factors such as PAX7, PAX6, AP-2 alpha and ISLET-1. In ...against new cancer biomarkers. We believe that repositories, such as Addgene and the DSHB, play an important... to ensuring affordable access to materials, repositories provide standardized quality-control and technical...to Our Guest Blogger!   David Soll has been the Director of the Developmental Studies Hybridoma Bank (DSHB...

  7. Addgene’s Top 10 Blog Posts from 2014

    Type
    Blog Post
    Published
    Dec. 16, 2014, 8:01 p.m.
    ...Mammalian Vectors By Marcy Patrick Will You Be My Mentor? Finding and Asking for Mentoring Support ...through the lens of Addgene’s blog and plasmid repository. Our blog is just over a year old, and it has...more and more scientists offering to share their stories – about their research, their job hunts, and their... Genes By Marcy Patrick Plasmids 101: Yeast Vectors By Marcy Patrick CRISPR-Cas9 FAQs Answered! ...

  8. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...proteins (FP) have also been important in mapping, monitoring, and manipulating neuronal circuits. Epitope ... is therefore difficult to use epitope tags to monitor single molecules - something researchers often ...tags, low FP expression may be insufficient for monitoring a weakly expressed POI. Spaghetti Monster - A... CA3 pyramidal neurons which are known to be notoriously difficult to label. It has also been shown that...technologies such as array tomography, super-resolution STORM imaging, and electron microscopy. Sample preparation...standard proteins tags like GFP. Specifically for STORM, the high intensity signal provided by smFPs can...Spaghetti Monster probes at Addgene Browse all AAV vectors Find ready-to-use AAV preps ...

  9. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type
    Blog Post
    Published
    Aug. 13, 2019, 12:58 p.m.
    ...Rogers is a postdoctoral researcher in Patrick Müller’s group at the FriedrichMiescher Laboratory of the Max... post was contributed by Katherine Rogers, a postdoctoral researcher at the Friedrich Miescher Lab of ... response to an experimental treatment can be monitored. This can reveal previously unknown functions ...splicing. (RNAi, in contrast, engages existing gene regulatory mechanisms to block activity and does not work...phiC31. The popular UAS/Gal4 and Cre/Lox gene regulatory systems have also been successfully implemented..."Single-cell reconstruction of developmental trajectories during zebrafish embryogenesis." Science 360.6392...

  10. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    Published
    Oct. 1, 2015, 2:30 p.m.
    ...reinforcement are ICSS and place preference.   Historically, ICSS was done by implanting an electrode and...successful inhibition of hippocampal neurons. Initial monitoring Second, this may sound rudimentary, but before...exciting—and unanticipated—road of discovery. Monitoring behavioral output The appropriate behavioral ...shock that has become associated with a visual or auditory cue. The hippocampus has long been known to be...versatility and standardization of genetic tools in the laboratory mouse, most optogenetics is performed in this...University of Michigan and he is currently a Postdoctoral Fellow in the lab of Mary Jeanne Kreek at the....    References 1. Stuber, Garret D., et al. "Excitatory transmission from the amygdala to nucleus accumbens...

  11. 500,000 Plasmids Shipped and Counting!

    Type
    Blog Post
    Published
    March 26, 2015, 3:24 p.m.
    ...Our plasmid repository succeeds because of our amazing community. The trust of our depositors to allow us...focused on our mission – documenting your plasmids, storing them, providing quality control and customer support...more scientists outside of the US now use the repository – about 50% of plasmids are shipped to labs outside...manage our website, and continue to improve the repository. We love being able to provide a useful (and ... us to help store and distribute their plasmids. The partnerships and collaborations with institutions...

  12. Make a Splash: Notions of Scientific Impact Are Evolving

    Type
    Blog Post
    Published
    Dec. 5, 2014, 2:54 p.m.
    ...Of course, all of you toiling away in laboratories this holiday season want the work you are doing to...been to publish in journals with a high “impact factor,” a measure that dates back to 1975 and is based... online access on scientific impact Partly, the story has to do with online accessibility. It is now about... recently reported that the most cited work in history is a 1951 description of an assay to determine ...of scientific publications. Ventures like Impact Story seek to find ways to capture scholarly work that... impact factor...

  13. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    Published
    Jan. 11, 2017, 8:47 p.m.
    ...of new tools came out including the single base editors, Casilio, CombiGEM, and a variety of pooled libraries...top requested plasmid from 2016 is an expression vector for CRISPR nuclease, SpCas9 from the Zhang Lab.... TLDR: This is a versatile mammalian expression vector that you can use to express both SpCas9 and your...resistance gene as separate peptides. PX459 This is the vector backbone. This particular backbone can be used ...Overall, this is a versatile mammalian expression vector that you can use to express both SpCas9 and your...mammalian cell lines. Alternative forms of this vector contain GFP-tagged and un-tagged versions of SpCas9...

  14. Tips for Getting a Faculty Position

    Type
    Blog Post
    Published
    July 20, 2023, 1:15 p.m.
    ...Structural Biology, and now serves as the Director of Student and Postdoctoral Programs at the Janelia Research...tribulations of the process, I’ve provided guidance and mentoring to several people that found my insights helpful...It helps to come from the lab of a well-known investigator. If you only have middle author papers or no... potential collaborations, critiques of your postdoctoral research, etc. The second question is whether...from Oregon Health Sciences University, did his postdoctoral fellowship with Jennifer Lippincott-Schwartz... His interests include the quality control of secretory proteins in the Endoplasmic Reticulum, optimization...

  15. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ...oligo when cloning my target spacers into the PX330 vector backbone? A2: There is no need to add the NGG PAM...degree of off-target effects depends on a number of factors, including: how closely homologous the off-target...recombination (HR) Q7: Can you summarize HR template vector design guidelines? A7: A few notes below are considerations...recently is the double nickase system, with PX335 vector, you need to find two guides for each cleavage ... co-deliver into the cells with the Cas9/sgRNAs vector (like PX330). For large changes (>100 bp insertions...typical size (~5 kb) donor plasmid with Cas9/sgRNAs vector, for one well in 24-well plate. A good example/...typical size (~5 kb) donor plasmid with Cas9/sgRNAs vector, for a one well in a 24-well plate. A good reference...

  16. Reflections and Looking Ahead

    Type
    Blog Post
    Published
    Feb. 25, 2025, 2:15 p.m.
    ...simplifies your viral vector options. Almost any AAV transfer plasmid in our repository can now be packaged...522 organizations in 41 countries enriched our repository with 15,020 new plasmids — a testament to the...shared progress. But numbers tell only part of the story. This year, we made significant strides in making... our collection, such as blogs describing viral vector serotypes, or hundreds of Data Hub reports detailing...innovation.  In response to a demand for more AAV viral vector options, we launched AAV Packaged on Request, a...

  17. SciComm with the Experts at Science in the News Part 2

    Type
    Blog Post
    Published
    April 27, 2017, 2:30 p.m.
    ...Chapter 6: The SITN Editorial Process -Vini discusses the importance of the editorial process and peer-to-peer...and training the next generation of science communicators. In this chapter we learn about some of their...social context. Episodes are driven by narrative and story telling. Chapter 5: The SITN Blog - Vini and Amy...graduate student at Harvard University, former Co-director of Science in the News, and member of the SITN...her PhD from Harvard University, is a former Co-director of Science in the News, and is a member of the...

  18. Supporting Rare Disease Research with BeHeard 2017

    Type
    Blog Post
    Published
    July 20, 2017, 2:30 p.m.
    ...molecules for use in the clinic and thereby restore motor neuron function in spinal muscular atrophy patients...gene results in the degeneration of spinal cord motor neurons. This disease is estimated to affect anywhere...other genes. He will use fluorescent proteins to monitor the localization of these genes, CRISPR to disrupt...Peritoneal Mesothelioma  Noushin Nabavi is a Postdoctoral Fellow at the Vancouver Prostate Center and ...systems should allow Nabavi and coworkers to test inhibitors of a variety of signaling pathways for their ...

  19. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    Published
    Feb. 18, 2016, 3:42 p.m.
    ...Your Insert and Vector by Gel Purification     Now that you’ve cut your insert and vector, unfortunately...unsuccessful 1), in a vector alone plate with many more colonies than the vector + insert plate (unsuccessful..., if you were cloning a gene into an expression vector, you would want the start of the gene to end up... ligation step. 3. Ligate Your Insert into Your Vector In the ligation step, you mix your purified, cut...Resources on Addgene.org Find the Perfect Empty Vector for Your Cloning Experiments Browse Our Molecular...

  20. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    Published
    Nov. 22, 2016, 3:30 p.m.
    ...Mary O’Riordan, and Victor Di Rita, and, last, homework assignments. The instructors are leaders in the...scientists. Over the course of the class, the moderator discussed individual sections of a paper, the ...quizzes and discussion on that week’s topic by the moderators. The assignments, which consisted of reading ...course was breaking up into focus groups with a facilitator. In the focus groups, we were required to critique... of the paper. They should be able to tell your story for you and they should also be easily understood...
Showing: 401 - 420 of 863 results