Optogenetics + CRISPR, Using Light to Control Genome Editing
Type
Blog Post
Published
Sept. 3, 2020, 12:15 p.m.
... had the lowest background activity in the dark state and highest fold induction at 31X. By using a slit...by blue light (470nm), paCas9-1 induced indel mutations via NHEJ (frequency of 20.5%) and induced modifications...significantly alter the system’s efficiency at generating mutations when activated with light and lowered background...