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Showing: 401 - 420 of 705 results

  3. Fluorescent Proteins 101: History of Fluorescent Proteins

    Type
    Blog Post
    Published
    Aug. 7, 2017, 1:58 p.m.
    ...the Pacific Ocean, off of the coast of Washington state. Using these samples, he was able to isolate two... protein’s structure and also discovered many mutations that alter and improve its function and shift ...

  4. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    Published
    Dec. 29, 2020, 2:15 p.m.
    ...this blog post. 9. Droplet Digital PCR for AAV Quantitation by Kate Harten DeMaio We made the switch to...blogger Shravanti Suresh who studies CRISPR at Iowa State University detailed five methods for using CRISPR...

  5. Developing Transferable Skills During Science Training

    Type
    Blog Post
    Published
    June 3, 2014, 2:14 p.m.
    ...you need to, go to ESL classes Practice your presentation skills (as much as you can), form a peer mentoring...content-useful website, write a blog Pursue speaking invitations by inviting others (be on a speaker committee...

  6. Why Do I Need an MTA Anyway?

    Type
    Blog Post
    Published
    May 1, 2014, 8:23 p.m.
    ...pride in facilitating the exchange of not just the physical samples, but also the legal documentation that...

  7. Let There Be LITE Plasmids

    Type
    Blog Post
    Published
    Nov. 21, 2013, 3:56 p.m.
    ...players in normal biological processes and disease states. While LITE plasmids have clear application to ...mammalian endogenous transcription and epigenetic states." Nature. 500, 472–476 (22 August 2013).   , ...

  8. Editor's Choice, September 2016

    Type
    Blog Post
    Published
    Oct. 7, 2016, 4 p.m.
    ...guest blogger Sean Stacey from Eastern Tennessee State University and provides a brief rundown of Sean’...phage are super cool and have considered getting a tattoo of my favorite molecular biology tool, P1 phage...

  9. Editor's Choice, August 2016

    Type
    Blog Post
    Published
    Sept. 2, 2016, 1:32 p.m.
    ...personal experience, is a quick way to generate many mutated plasmid constructs. The second post was contributed...specificity issues, and other means of generating mutations in live cells are all discussed further in this...

  10. Management for Scientists: Seeking Feedback

    Type
    Blog Post
    Published
    Jan. 8, 2015, 2:34 p.m.
    .... Check out Joanne's Reddit AMA Here they are annotated with my comments: Everyone on the team talks...complaining. They may worry that it seems like "tattling" if others are involved. They may feel they are...

  13. What's New in CRISPR - September 2019

    Type
    Blog Post
    Published
    Sept. 10, 2019, 12:56 p.m.
    ... Using the structure of CasX at eight different states of assembly from cryo-electron microscopy, the ...activation and genome editing by fusing Cas9 to a mutated human estrogen receptor. This protein fusion stays...

  14. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    Published
    June 2, 2022, 2:15 p.m.
    ...wanted to ensure applicants understood what the expectations were for the intended position. After submitting...Addgene 101” series, where each team gives a presentation on what they do and how it relates to other ...

  15. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    Published
    Dec. 12, 2023, 2:15 p.m.
    .... The Patriarchi Lab aimed to overcome these limitations by developing and depositing two new norepinephrine... using ColabFold (Mirdita et al., 2022). B, Representative images of HEK293T cells and neurons expressing...

  17. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    Published
    April 1, 2014, 5:33 p.m.
    ...different ways to succeed – as loner or gregarious;  dictator or laissez-faire; theory or practice; business-like...clear and attractive reports, being good at presentations and of course, networking and collaborating....

  18. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

    Type
    Blog Post
    Published
    Feb. 20, 2014, 4:57 p.m.
    ...the process, that you are not losing library representation.” It’s also important that you infect enough...selection. Shalem and Sanjana say they kept a high representation of library within cells and aimed for 300 cells...

  19. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    Published
    Feb. 14, 2019, 2:35 p.m.
    ...drop off the box at any location where the United States Postal Service picks up mail. NEB actually started...drop off the box at any location where the United States Postal Service picks up mail. Recycle plastic...

  20. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    Published
    May 18, 2017, 2:30 p.m.
    ...biosensors track cellular characteristics like pH, redox state, and voltage across membranes. These sensors generally... experimental setup? Consider what kind of instrumentation you have available, and the conditions of your...
Showing: 401 - 420 of 705 results