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We narrowed to 806 results for: Tes

Showing: 401 - 420 of 806 results

  1. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    Published
    June 10, 2025, 1:15 p.m.
    ...sequences into genomic DNA downstream of gRNA-specified sites. Created with BioRender.com. CASTs quickly became...mutations require individualized optimizations, safety testing, and approval. In contrast, a system that can deliver... the National Academy of Sciences of the United States of America, 114(35), E7358–E7366. https://doi.org...

  2. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    Published
    Nov. 1, 2016, 2:30 p.m.
    ...sensitive restriction enzyme DpnI to remove the templates. Digestion products are then transformed directly...that the exonuclease activity of the polymerase generates sticky ends in the PCR reactions. These sticky...time to completely digest the template DNA. Note—templates must have been isolated from methylation competent...

  3. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    Published
    Aug. 19, 2014, 3:39 p.m.
    ...carry in the target cell. These vectors allow you to test the function of Your Gene Of Interest (YGOI) in ...collection of empty backbones that have been designed, tested, and published by academic scientists. To help ...a mutated strain or a mutated version of YGOI to test the different phenotypes of the specific mutations...

  4. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    Published
    April 15, 2014, 3:11 p.m.
    ... can’t safely recycle these materials either.  Test tubes aren’t cheap everywhere Lab supplies and equipment...thrifty streak.  One of the most accessed blogs on BitesizeBio is the protocol for making your own Taq polymerase...I got good at this despite the fact that my lab mates thought this was hilarious.  Birth of the baby gel...

  5. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    Published
    Jan. 16, 2014, 2:56 p.m.
    ...labs have websites listing alumi and it is easy to use publication records, university websites, ResearchGate... lunch or beer) and in 1:1 conversations. This creates a level of familiarity and confidentiality to ensure...

  6. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    Published
    May 9, 2019, 1:15 p.m.
    ...cell type that can differentiate into hepatic or intestinal cell types. MEFs were reprogrammed into iEPs ...least day 13. However, distinct transcriptional states were seen as early as day 6. All together these...in a competitive transplant assay in the mouse intestine, demonstrating its utility in long-term tracking...

  7. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type
    Blog Post
    Published
    Sept. 17, 2020, 1:15 p.m.
    ...building that someone from a different company had tested positive for COVID-19 (it turned out to be an unfounded...operations until our normal work resumed. Addgene distributes and fulfills requests for scientists doing almost...type of biomedical science research. Our work accelerates and enhances productive collaborations as well...

  9. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    Published
    Sept. 26, 2017, 12:12 p.m.
    ...is mind-boggling. Unlike a mountain – with many routes up and only one destination – a career in the sciences...molecular pathways, I found myself curious about other routes – In terms of both types and topics of work. A ...rare organisms so I found it hard to stick to vertebrates alone.  And a longstanding inclination to be ...

  10. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    Published
    Jan. 25, 2018, 2:09 p.m.
    ...Avidin and its derivatives have four biotin-binding sites per molecule so, theoretically, saturated avidin...were unable to cleave TEV within the protein we tested, it may be possible with other fusion proteins ...bacteria significantly enhance protein folding (tested using real science). Make a stock solution of 2.5...

  11. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    Published
    July 21, 2022, 1:15 p.m.
    ...scicomm group project on the Leith river. Photo courtesy of Leonardo Ramos.  I enrolled into a summer...industry. Along this road, science communication graduates are often well-equipped. Many institutions are...explores risk-taking behavior among collision sport athletes, as well as the ethical and health policy implications...

  12. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

    Type
    Blog Post
    Published
    May 25, 2021, 2:29 p.m.
    ...have recently received a grant from the National Institutes of Health that will address this problem.The ...animals may be variable among animals and bleed dates. The development of monoclonal antibody (mAb) technology... represent the official views of the National Institutes of Health.     References and Resources References...

  13. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    Published
    Dec. 19, 2019, 2:40 p.m.
    ...avoid off target photostimulation of axons and dendrites on neighboring neurons. We have also packaged ... AAV in smaller aliquots of 20 ul for serotype testing to help you pick the best AAV for your experiment...control plasmids in new serotypes. See our Serotype Testing AAV inventory for the entire collection! Plasmid...

  14. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

    Type
    Blog Post
    Published
    Nov. 12, 2019, 2 p.m.
    ...physical and chemical conditions like YNP thermal sites - are also of practical interest since they’ve previously...distance. At each collection site, we took GPS coordinates, water temperature readings using a heat gun,...the van der Oost lab. Phylogenetic comparison indicates that like GeoCas9 and ThermoCas9, IgnaviCas9 is...

  15. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type
    Blog Post
    Published
    Dec. 13, 2018, 1:14 p.m.
    ...shown that cells treated with sucrose or other osmolytes to induce hypertonicity display enhanced monoclonal...impaired endocytosis. In parallel assays designed to test the endocytosis of a fluorescent dye and transduction...host of other AAV serotypes; it will be exciting to test this approach with different capsids. Most importantly...

  16. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    Published
    Oct. 29, 2015, 2:30 p.m.
    ...of heredity were thought of as everything from parasites, to symbionts, to genes and the labels applied... the National Academy of Sciences of the United States of America 110.39 (2013): 15521–15529. PMC. PubMed... the National Academy of Sciences of the United States of America 70.11 (1973): 3240–3244. PubMed PMID...

  17. Celebrate National Book Month with Addgene

    Type
    Blog Post
    Published
    Oct. 3, 2019, 12:47 p.m.
    ... to read a little more this October. Science favorites Musicophilia: Tales of Music and the Brain by ...Communications and Marketing Coordinator Non-science favorites  The Mistborn series by Brandon Sanderson "The... reader of historical fiction (I can personally attest to this!). Following history from the Ice Age to...

  18. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

    Type
    Blog Post
    Published
    July 21, 2020, 1:15 p.m.
    ...measures these absorbances using UV-transparent cuvettes. First you measure the absorbance of the buffer...-2 ul of sample and the run time is just a few minutes per sample. During the run, DNA fragments move ...quantified over time using a fluorescent dye that intercalates into the DNA such that smaller fragments are...

  19. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type
    Blog Post
    Published
    July 27, 2017, 1:11 p.m.
    ...variety of research applications and particularly notes their ability to be precisely spatio-temporally ...down the HMO of interest, the lactose released activates expression of GFP from the lac operon. GFP expression...Light to Control CRISPR Genome Editing Choose the Brightest Fluorescent Protein for Your Experiments Resources...

  20. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    Published
    July 17, 2018, 12:23 p.m.
    ...the selected card on the board game. They had 35 minutes to develop a strategy and build a transfer plasmid... manipulate human stem cells. Then they had 10 minutes to expose their strategy, justifying each single...lentivirus-based ex-vivo gene therapy’ strategies to undergraduates, however you may use it and modify it for non-commercial...
Showing: 401 - 420 of 806 results