Skip to main content
Addgene
Showing: 401 - 420 of 621 results

  1. A Control for All Seasons

    Type
    Blog Post
    Published
    Oct. 5, 2023, 1:15 p.m.
    ...knockout.  If knockout samples are unavailable, try substituting with knockdown approaches using siRNA or shRNA...either by modifying the blocking conditions or by substituting for a more specific secondary antibody. The ...

  2. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    Published
    July 28, 2016, 2:30 p.m.
    ...only semi-quantitative. It won’t tell you the distribution of states in a sample; instead, it will suggest...the Lu Lab at MIT. He is broadly interested in biocomputing and biological circuits.     References 1. N...

  3. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ...Wenning Qin and Haoyi Wang. CRISPR/Cas9 is revolutionizing the mouse gene-targeting field. Mice have long... he is interested in using CRISPR to develop therapeutic applications that better human health.     References...

  4. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    Published
    March 6, 2018, 1:49 p.m.
    ...and circularization of dsDNA (6). Less commonly utilized in vitro, Taq DNA ligase will ligate only nicks...replacements for T4 DNA Ligase in applications such as routine cloning or DNA library preparation. However, when...

  5. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    ...to generate a monoclonal cell lines by limiting dilution. The rest of the cells were frozen down. ...Isolating a Monoclonal Cell Population by Limiting Dilution Lentivirus Production Websites Primer3: for ...

  6. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    Published
    Oct. 27, 2020, 1:15 p.m.
    ...techniques It is fair to say that CRISPR has revolutionized molecular biology and has enabled functional.... Nevertheless, amiRNA-based RNAi experiments routinely display highly specific and robust gene silencing...

  8. Finding Your Science Policy Path

    Type
    Blog Post
    Published
    Sept. 22, 2020, 1:15 p.m.
    ...legislation (see Figure 1 for career map). The beautiful part of science policy is that you can jump from...work can happen at the national level within the executive branch or in Congress, or can also happen at the...

  9. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ...reference library. If this happens to you, one solution is to identify all the alternative names for your...screen identifies genetic vulnerabilities and therapeutic targets in acute myeloid leukemia." Cell reports...

  10. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ...that can be inserted into the target protein. The utility of these reagents is that binding is covalent, ...Observation of intersubunit movement of the ribosome in solution using FRET." Journal of molecular biology 370.3...

  11. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    ...The first base editors revolutionized CRISPR gene editing. Cytosine base editors (CBEs) and adenine base...than an accessible/inaccessible binary. A word of caution: CGBEs and indels Cytosine transversion editors...

  12. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...methanol, PFA-saponin, PFA-triton). The fix/perm solutions can make a significant difference in the efficacy...and methanol, we found that the optimal perm/fix solution depended on the specific antibody:target combination...

  15. New FLIS Plasmid (April Fools 2022)

    Type
    Blog Post
    Published
    April 1, 2022, 1:15 p.m.
    ...and re-contain any specimens that escape during routine lab activities.  Here, we demonstrate the new system...

  18. New Videos: Addgene Lab Tips

    Type
    Blog Post
    Published
    Feb. 15, 2018, 2:41 p.m.
    ...several times. This will help ensure ensure equal distribution. Lab tip #3: Barcode everything Use barcodes...
Showing: 401 - 420 of 621 results