We narrowed to 635 results for: UTY

...biotinylating your macromolecule of interest To utilize the natural binding of biotin and avidin, macromolecules...biotinylation reagents to help with the specific elution of biotinylated proteins. For help determining ...

...interview and sell a vision for your contribution to a Department's/Institution's success is the task. Do your...

... genes with potentially harmful mutations in therapeutic settings. Silencing of a target gene is achieved...the backbone, shRNAs are most often expressed constitutively, either in lentiviral or retroviral backbones...

... is a nearly ubiquitous tool, but proceed with caution! Loading controls only address one type of error...by sample concentration as well as by antibody dilution — both factors we’ve covered in our previous blog...

...graphing data, choosing controls, or mixing clear solutions—public speaking is skill that any scientist can...What happens next?”  Describe how you pushed to a solution or overcame obstacles. Take time to consider the...

...demonstrated two potential strategies for CRISPR-HIV therapeutics - one using dCas9-SAM to activate HIV-1 transcription...next few years, as it would open up many new therapeutic possibilities for some of the world’s most common...

... in the middle of your insert. Gateway cloning utilizes λ integrase to catalyze directional cloning of...Golden Gate cloning methods. Whether it is for routine cloning, assembly of multiple fragments, or synthetic...

... for i-GONAD. A micropipette is used to inject solution into the oviduct wall between the ampulla and ...incision that exposes the ovaries and oviducts. After solution injection into the oviductal lumen, tweezer-type...

... percentage of acrylamide. To further improve resolution, use a stacking gel on top of the resolving gel...Gradient gels are another great option to enhance resolution. In this setup, gels have an increasing range...

...modification was impossible. With the genome engineering revolution came epigenome-engineering tools - zinc finger...transduced cells. Grant Challen’s lab also created constitutive (pCMV-dCas9-D3A) and Tet-dependent (TetO-dCas9...

...knockout.  If knockout samples are unavailable, try substituting with knockdown approaches using siRNA or shRNA...either by modifying the blocking conditions or by substituting for a more specific secondary antibody. The ...

...them? Or maybe you just want to make some really beautiful images? Multiplex IF may be just what you need... L., Zhu, Y., Neumann, E., De Caestecker, M., Gutierrez, D., & Spraggins, J. (2022). Multiplex Immunofluorescence...

...SV40, are always active and thus referred to as constitutive promoters. Others are only active under specific...that also express GAL4. Usage of this system revolutionized genetic studies in flies. An additional component...

...only semi-quantitative. It won’t tell you the distribution of states in a sample; instead, it will suggest...the Lu Lab at MIT. He is broadly interested in biocomputing and biological circuits.     References 1. N...

...Wenning Qin and Haoyi Wang. CRISPR/Cas9 is revolutionizing the mouse gene-targeting field. Mice have long... he is interested in using CRISPR to develop therapeutic applications that better human health.     References...

...and circularization of dsDNA (6). Less commonly utilized in vitro, Taq DNA ligase will ligate only nicks...replacements for T4 DNA Ligase in applications such as routine cloning or DNA library preparation. However, when...

...techniques It is fair to say that CRISPR has revolutionized molecular biology and has enabled functional.... Nevertheless, amiRNA-based RNAi experiments routinely display highly specific and robust gene silencing...

...airflow before and while you are working. Perform routine checks for mycoplasma contamination, before using...importance of preventing contamination. Be aware and cautious rather than learning it the hard way! Many thanks...

...legislation (see Figure 1 for career map). The beautiful part of science policy is that you can jump from...work can happen at the national level within the executive branch or in Congress, or can also happen at the...

...to generate a monoclonal cell lines by limiting dilution. The rest of the cells were frozen down. ...Isolating a Monoclonal Cell Population by Limiting Dilution Lentivirus Production Websites Primer3: for ...