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Showing: 401 - 420 of 633 results

  1. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...IPI’s recombinant version is a fusion of mouse variable and rabbit constant domains and is recommended...Tension Sensor (Zhong et al., 2024). In this new variation on their previous sensor STReTCh, the 11-amino...

  2. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    Published
    July 30, 2024, 1:15 p.m.
    ...save researchers significant time and removes a variable from the lengthy process of validating and using... (Figure 4), our catalog includes vectors in a variety of serotypes, including the retrograde AAVrg and...

  3. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    Published
    June 20, 2024, 1:15 p.m.
    ...way for the cell to control DNA replication. A variety of other proteins also contribute to this regulation...emboj/17.17.5201 del Solar, G., Giraldo, R., Ruiz-Echevarría, M. J., Espinosa, M., & Díaz-Orejas, R. (1998...

  4. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...domain conferred several new improvements. The new variant has increased signal-to-noise ratio and better ...disease-relevant large proteins. They demonstrated REVeRT in various contexts in vitro and in vivo, including in human...

  5. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ... Cas9 activator should I use? There are a wide variety of activators you can use for your experiments....Addgene Blog Learn how to Choose the Best Cas9 Variant for Your Next Experiment Find out More about Genome-wide...

  6. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...expression? Low expression outcomes can result from variety of sources, so fear not—there are a few simple ...up a small-scale expression experiment to test variables such as temperature, time, and media conditions...

  7. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    Published
    March 27, 2018, 1:32 p.m.
    ...genome editing. In this post we'll walk through a variety of ways you can use AAVs to improve your genome...being the vector of choice for gene delivery to a variety of cell types, now you know that AAVs can provide...

  9. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ...homology arm, but keep in mind these numbers may vary based on your locus of interest and experimental...ssODNs in mouse models. Although HDR efficiency varies across loci and experimental systems, ssODN templates...

  10. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    Published
    March 28, 2024, 1:15 p.m.
    ...are chimeric antibodies composed of rat or mouse variable regions fused with rabbit constant domains. All...s work with the Boston Children’s Hospital and Harvard-based lab of Tim Springer, the founder of IPI. ...

  11. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    Published
    Feb. 8, 2018, 12:17 p.m.
    ...and red fluorescence. The paper reports a wide variety of applications of the Enhanced Nano-Lanterns including...PMC4942401. 5. Susuki, K., et al. (2016) Five colour variants of bright luminescent protein for real-time multicolour...

  12. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ...to the intended edit rather than some passenger variant that came along with the single cell clone (although...SaCas9, NmeCas9, Cas12a enzymes, and engineered variants thereof offers additional PAM options that can...

  13. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ...demethylation and induce transcription at CpG regions of various promoters. Other researchers additionally fused...applications direct catalytically dead dCas9 fused to a variety of epigenetic modifiers to specific loci without...

  14. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...responsive to light in combination with retinal) variations designed to give researchers flexibility in using...photoswitches can be used in a single pathway to activate various protein regulators at different points in the pathway...

  15. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    Published
    June 8, 2021, 1:15 p.m.
    ...investigator (PI) Runs the lab Writes a lot of grants Varying degrees of engagement with what happens in the ...Undergraduate student Roles and responsibilities can vary "Lab assistants" usually wash glassware, prep media...

  16. Finding Your Science Policy Path

    Type
    Blog Post
    Published
    Sept. 22, 2020, 1:15 p.m.
    ... worked for seven different organizations in a variety of science policy roles including fellowships, ...folks who work in science policy come from a wide variety of backgrounds with technical expertise that ranges...

  17. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ...curing diseases caused due to single nucleotide variations in the genome.    Due to the rise of CRISPR in...engineering and open advertisement of the technology on various non-scientific platforms, CRISPR has also transcended...

  19. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...et al. A percentage is not listed because many variables (cell type, criteria used, etc.) can effect reprogramming...insertional mutagenesis and random integration creates variation between iPSC clones. Retroviruses are not considered...
Showing: 401 - 420 of 633 results