We narrowed to 460 results for: abo.3

... covered. Happy voltage sensing!   Figure 3: Components of the types of voltage indicators reviewed...especially essential. If you want to learn more about how viruses can be used for chemigenetics, biosensors...

...European ancestry.  Understanding the impact Fig. 3: Histograms indicating the frequency (x-axis) which...dependencies associated with each ancestry group. Since about 90% of the cell lines used in DepMap CRISPR screens...

...school. The transition has been a bit rough for her (3 weeks in and there's a big office vs. lab culture ...scientist anymore. Being a scientist is not about what you do--it’s about how you think. I have not worked at ...10 years, but when people ask me about my job, I have no qualms about saying “I’m a scientist.”    What...installment of the Addgene career guide, we’ll think about what causes this culture shock and how to overcome...counterpoints to some of the causes of culture shock above, but after polling some Addgene colleagues, we’ve...This is probably because you don't have to worry about experiments taking longer than expected or dealing... hour days at the lab, I encourage you to think about how much of that time you’re actually spending doing...

...use this ID to name the corresponding Excel sheet.3. Be specific  - When assigning a physical location...is the Laboratory Operations Manager at Addgene.  In addition to helping with daily laboratory operations...through barcoded tubes that are scanned into our Laboratory Information Management System (LIMS), a database...available for distribution!    Check Out How We Think about Developing Lab Management Software    The plasmid's...the correct sample. Tracking samples - it's all about location, location, location There are many different...of these samples have the 2D barcodes mentioned above. These barcodes identify what type of sample is ...every barcode on every tube contains information about that tube’s appropriate location. The barcode also...

...is spent trying to raise the alpha isoform of GSK-3 to the same (deserved) stature as the beta isoform...fraction (<1%) of authors have asked for advice about the scope of the policy. End of story? Not quite...the data policy was raised by researchers worried about the effort to make the underlying data easily available...making data available, but there remains concern about the extra work this may entail.  So if access to...be archived? Simplifying data access Some of the above mentioned issues could be mitigated by on-line services...him on Twitter @jwoodgett.     More Discussions About Data Sharing: PLOS Data Sharing Policy Discussion...Scholarly Kitchen) Read more Addgene blog posts about scientific sharing ...

...Strong yeast expression promoter from glyceraldehyde 3-phosphage dehydrogenase Constitutive  Very strong,...Promoter of the arabinose metabolic operon Inducible by arabinose and repressed catabolite repression in the ...Browse All Plasmids 101 Posts Learn about Mammalian Vectors Read about Reporter Gene like Luciferase and...and operators (response elements). Promoters are about 100 to 1000 base pairs long and found upstream of...this list is a great place to start, the tables above do not delve into the tissue or development-specific...

...Magnetogenetic Ion Channels. Biophysical Journal, 116(3), 454–468. https://doi.org/10.1016/j.bpj.2019.01.003...our tissues unimpeded. Andrew and Maria dreamed about the possibilities of a magnetoresponsive fluorescent...of improved response. Then, just when Andrew was about to suggest she deprioritize the project, Maria’s...with a strong commitment to exploration and collaboration. Andrew is a physicist with a background in ...potential applications.  Andrew is a frequent collaborator and is happy to give advice and support for ...interested in working on these questions or collaborating, the York Lab is eager to hear from you. Find...

...acceptable. Aims should be mechanistic, doable by 1-3 people in 1-5 years, and contribute to resolving the...a single grant. Either way, think realistically about the time and resources required to feasibly accomplish...interrupted frequently by committee members. As mentioned above, plan to give your 10 minute overview and address... list of common questions, check out this eBook about applying for faculty positions. You need to practice...program. You should be proud of it and be excited about getting the opportunity to realize your vision. ...blog posts Resources on Addgene.org Learn more about careers at Addgene Watch our career videos ...

...100th birthday, as your mom will never forgive you… #3 Honor the work day and keep it holy Keep a regular...on the Society for Laboratory Automation and Screening (SLAS) Electronic Laboratory Neighborhood.   Work...worry less about how they will handle an emergency and this will make you much more confident about their ...

... using AAV-PHP.B vectors? See Supplementary Table 3 in Huang et al. for a list of PHP.B permissive and... an AAV rookie  Learn about some considerations when using AAVs Learn more about using AAV as a tool for...PubMed PMID: 29175632. If you want to learn more about how PHP.B and PHP.eB were engineered, check out ...Additional resources on the Addgene blog Learn more about PHP.eB, and PHP.S, a peripheral nervous system (...

...connectivity to other tools used in research; and (3) connectivity to open source data repositories. To...RSpace is integrated with most of the tools noted above, and has a modern API. Integrations with additional...into RSpace. The kind of informal conversations about research that can take place in Slack normally never...RSpace documents to external data sources (described above) further enhances the value the repository integrations...Minisymposium on Reproducibility Read all blog posts about scientific sharing Resources on Addgene.org Find...

...Government (2016). 2. CDC Information Pages on Antibiotics 3. WHO Antimicrobial Resistance Fact Sheet  Additional...resistant infection or not, because scientists in the laboratory will need to isolate and grow whatever bacteria...method that is frequently used in the clinical laboratory. What can we do to stop the emergence of antibiotic...CDC. Antibiotic resistance can be scary to think about, but we can all do our part to learn all we can,...drug resistance. Additionally, she is passionate about health equity, science communication, and public...

...sample to a mastermix. At Addgene, we typically load 3 dilutions onto our ddPCR plate. As the titer is unknown... droplet generator, you will have to be careful about transferring your droplets from the droplet generator...Additional resources on the Addgene blog Read more about polymerase chain reaction Browse our blog posts ...collection of viral vector related protocols Learn more about the Addgene viral service ...

...Orthogonal Type VI-A CRISPR-Cas Enzymes." Mol Cell. 66(3) (2017):373-83. PubMed PMID: 28475872. Find plasmids...CRISPR Featured Topic Page Learn about RNA Targeting with Cas13a Learn about CRISPR DNA Base Editing Resources...and sequences that can be edited.   As discussed above, the ability to edit RNA has multiple advantages...

... the subsequent bbmerge process.     Fig. 3: How we get bbdone with our bbduk process.    ...much information as possible from depositing laboratories and associated publications, but our quality...the process. Another design choice we appreciated about Nextflow was how effortless it was for us to switch...interface. In our next post, we’ll share our story about how we used Nextflow Tower to augment our pipeline...

...specifically to the blue triangle protein but not the other. 3) The beads are collected by centrifugation or a magnet...proteins or nucleic acids. Read on to learn more about this versatile technique.  Immunoprecipitation ...proteins in an IP are not denatured (learn more about antibodies here!) When using Protein A/G to immobilize...eluted by boiling the beads in SDS. (To learn more about Western blotting check out our blog post The Basics...

...often be seen by sinking of the tissue (see Figure 3). This method is probably the most scalable method...Generating a transgenic plant takes even longer, is more labor intensive, inefficient, and often not reproducible... region is transferred into the plant. In the laboratory we can utilize this natural process by replacing...system. The vectors mentioned in this blog post above are ready to use vectors, but with the MoClo system...

...darker than full vector particles.     Figure 3: Electron micrograph of AAV vector preparation shows... before being distributed to customers. Details about our production protocols, titering methods, and ...either in-house by Addgene scientists or through collaboration with viral vector manufacturing facilities, ...generated from lentiviral preparations (described above) that are subject to a concentration step prior ...

...www.businessinsider.com/how-to-get-the-most-out-of-job-fairs-2014-3 https://www.forbes.com/sites/neilpatel/2015/05/21/...or postdoc forever, and you need to be thinking about what comes next. To that end, one of the most important...personal connection; the importance of which will be elaborated on later. As for what job fairs to consider, ...the specific position he or she originally called about, you can keep in touch and reach back out when you...

... sequence complementary to the mRNA sequence. The 3’ end of  the tRNA binds the amino acid that corresponds...usage bias Read more about plasmids in our Plasmids 101 blog series Learn more about trends in synthetic... out Addgene's Molecular Biology Reference Read about genetic code expansion Browse our plasmid collection...