We narrowed to 512 results for: ato
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TypeBlog PostPublishedMarch 24, 2020, 1:15 p.m....lysis. This can be challenging depending on your laboratory situation or tissue collection method, so biotechnology...
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Transferable Skills Guide: Cross-team Communication
TypeBlog PostPublishedNov. 21, 2019, 1:53 p.m.... a lot on context. New people in the lab or collaborators will not have the context you do, which means... -
Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number
TypeBlog PostPublishedSept. 4, 2018, 12:53 p.m....of IPTG. Because RepA is a plasmid replication initiator protein, they could alter copy number of the plasmid... -
RaPID Detection of RNA-protein Interactions
TypeBlog PostPublishedAug. 16, 2018, 12:40 p.m....one to study RNAs expressed from their native regulatory elements. Controls for RaPID experiments RaPID... -
dTAG - You're it!
TypeBlog PostPublishedJune 21, 2018, 2:06 p.m....dTAG-47 Weintraub et al. YY1 Is a Structural Regulator of Enhancer-Promoter Loops. Cell. December 14 ... -
Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics
TypeBlog PostPublishedNov. 3, 2020, 2:15 p.m....makes them light-responsive. When light shines on excitatory channelrhodopsin-expressing neurons, the channelrhodopsins... -
Summer SciComm: Preprints
TypeBlog PostPublishedAug. 16, 2022, 1:15 p.m...., social media, coffee chats with potential collaborators, blogging and more. These each have different... -
Summer SciComm Series: Cognitive Load
TypeBlog PostPublishedJune 16, 2022, 1:15 p.m....help: If there’s a teacher, mentor, or other educator working with your audience, ask! They are experts... -
Antibodies 101: Affinity Reagents
TypeBlog PostPublishedApril 14, 2022, 1:15 p.m....antibody constant region. They are generated using combinatorial libraries, and high specificity has been reported... -
Human Germline Editing Using CRISPR
TypeBlog PostPublishedAug. 10, 2017, 2:19 p.m....proceed with caution and with proper oversight from regulatory and ethics committees comprised of individuals... -
Lighting Up Cell Signaling with Photoswitchable Kinases
TypeBlog PostPublishedJuly 25, 2017, 1:20 p.m....endogenous ERK, and its localization serves as an indicator of MEK1 activity. When MEK1 is active, it phosphorylates... -
Addgene's Tips for Plasmid Quality Control
TypeBlog PostPublishedJan. 14, 2016, 3:30 p.m.... (such as LTRs or ITRs), hairpins, or strong terminators. Detailed instructions on other factors to consider... -
Plasmids 101: Golden Gate Cloning
TypeBlog PostPublishedAug. 27, 2015, 2:30 p.m....for multiple DNA components (promoters, genes, terminators, etc) to be assembled in the correct order in... -
Career Coaching for Scientists: Why and Where Do I Find One?
TypeBlog PostPublishedJan. 29, 2015, 1:25 p.m....help you. A good coach usually will be a clear communicator and make direct suggestions for improvement ... -
5 Steps to Writing Grant Proposals for Grad Students
TypeBlog PostPublishedFeb. 11, 2014, 12:13 p.m....first. Additionally, reach out to peers and collaborators who have succeeded at grant writing to see what... -
Plasmids 101: The Wide World of Natural Plasmids
TypeBlog PostPublishedOct. 8, 2024, 1:15 p.m....Auriol, P., Vogel, T. M., & Simonet, P. (2001). Laboratory-scale evidence for lightning-mediated gene transfer... -
Making CRISPR Plasmids Using Fragmid
TypeBlog PostPublishedAug. 29, 2024, 1:15 p.m....Plasmid pRDB_053 (for transgenic flies; gypsy insulator sequences; white + selectable marker; attB site... -
Viral Vectors 101: Chemogenetics
TypeBlog PostPublishedJuly 11, 2023, 1:15 p.m....PSAMs PSAMs PSAMs, or pharmacologically selective actuator modules, were developed with the goal of creating... -
Viral Vectors 101: Virus Safety
TypeBlog PostPublishedJune 22, 2023, 1:15 p.m...., some adenovirus strains are known to cause respiratory diseases in humans, earning this virus a BSL-... -
Plasmids for Endogenous Gene Tagging in Human Cells
TypeBlog PostPublishedApril 6, 2017, 1:02 p.m....specific, individual proteins under endogenous regulatory control. But even with the advent of CRISPR/Cas9...