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We narrowed to 803 results for: cha;

Showing: 401 - 420 of 803 results

  2. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

    Type
    Blog Post
    Published
    Oct. 20, 2020, 1:15 p.m.
    ...independently” and “the power or opportunity to act or take charge before others do”. Candidates who have “started...including those that find events stressful. Video chatting makes it pretty easy to make 30 minute appointments...being able to just look at a paragraph and do a character count in your head.  Here are some sites that ...

  3. Summer SciComm: Science Storytelling

    Type
    Blog Post
    Published
    Sept. 16, 2022, 1:15 p.m.
    ...one or two roadblocks that sparked creativity, changed the outcome of the project, or illustrates the ... and what can be left out, which can be a big challenge!  The resolution Throughout your conflict, you... and driver of your story gives your audience a chance to get curious and invested in the research itself...

  4. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    Published
    June 9, 2016, 2:30 p.m.
    ...against invading DNA. In 2014, Swarts et al. characterized Argonaute from Thermus thermophilus (TtAgo),...expressed from a plasmid or in vitro transcribed, and changes in gRNA secondary structure can affect editing ... 3. Swarts, Daan C., et al. “Argonaute of the archaeon Pyrococcus furiosus is a DNA-guided nuclease that...

  5. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    Published
    March 6, 2014, 7:12 p.m.
    ...you don’t take this past the chatting stage you will rarely make any change in your skill sets or activate...formality to mentoring Just getting together and chatting with another person about their career path is...

  7. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    Published
    April 4, 2023, 1 p.m.
    ... Ploidy Usually diploid, but spontaneously changes Usually haploid Usually haploid Pathology...their peroxisomes will proliferate upon a media change into methanol and peroxisomal autophagy can be ...Resources References Liu, Wei, et al., From Saccharomyces cerevisiae to human: The important gene co‑expression...

  8. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ...Susanna Stroik The Doudna lab’s recent work characterized a new tool for RNA targeting, with a leg up ...were compared head-to-head for gene expression changes of non-target genes and RNA-seq of a nuclear (MALAT1...denaturing conditions, high temperatures, and other chaotropic conditions better than most FPs. The group also...

  9. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    Published
    April 19, 2017, 3:25 p.m.
    ...and accurate sequencing (“reading”) of DNA, have changed our collective thinking about the feasible size...seqWell, Inc., our mission is to overcome crucial challenges in NGS by developing technologies that can help...problem in multiplex NGS library preparation. The challenge here is that, as more samples are sequenced simultaneously...

  10. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    Published
    April 7, 2022, 1:15 p.m.
    ...fusion protein binds to glutamate, conformational changes lead to an increase in fluorescence intensity from... from the inserted GFP. After characterizing iGluSnFR in vitro, showing that it is selectively activated...response than its predecessors. The detailed characterization of the new variants is available here. Upgrades...

  11. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

    Type
    Blog Post
    Published
    July 21, 2020, 1:15 p.m.
    ...absorb best at 280 nm and organic compounds and chaotropic salts maximally absorb at 230 nm. The A260/A280...doesn’t tell you about contaminating proteins or chaotropic salts. Capillary electrophoresis Quantifying...fragments move through a micro- or nanofluidic channel and the sample is separated by electrophoresis....

  12. DAP arrays for multiplex gene editing

    Type
    Blog Post
    Published
    July 7, 2022, 1:15 p.m.
    ...CRISPR tools have leveraged such host-defense mechanisms to enable multiplex gene editing in a variety... fused base editors (nCas9-BE), as Cas9 is mechanistically more favored for efficient base editing. They... To do so, they harnessed the tRNA processing mechanism, which is conserved in all living organisms and...

  13. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    Published
    Aug. 13, 2015, 2:30 p.m.
    ...throughput functional characterization of specific edits to the genome. Such characterization can be done in ...The RNAi process had just been discovered, the mechanism was barely understood.” Doench’s graduate work...

  14. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    Published
    Dec. 18, 2014, 4:06 p.m.
    ...non-academic one, one of the biggest changes (and perhaps challenges) is the need to present yourself using...studied astronomy or bioinformatics data, then the chance is that you have handled large data sets and are...

  15. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    Published
    Oct. 9, 2014, 3 p.m.
    ... for them to be imaged in distinct fluorescent channels on the microscope. With the proliferation of fluorescent...the best protein for a given application is a challenge. To help keep you abreast of the latest fluorescent... abundant proteins, with the blue and infrared channels used for more abundant proteins or marking compartments...

  16. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ...cells Blocks polypeptide synthesis at 80S; inhibits chain elongation 100-800 ug/mL Hygromycin B hygB HeLa...cells Blocks polypeptide synthesis at 80S; inhibits chain elongation. 50-500 ug/mL Puromycin pac HeLa, 293HEK...Jurkat T cells Inhibits protein synthesis; premature chain termination 1-10 ug/mL Zeocin Sh bla HeLa, NIH3T3...

  17. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type
    Blog Post
    Published
    Jan. 21, 2025, 2:15 p.m.
    ...management, the journey can be as exciting as it is challenging. How do you prepare for this shift and navigate...experiences, and practical advice for overcoming challenges. Experimenting with leadership opportunities...'ve witnessed several colleagues do this while changing industries and positions. Some people discover...

  18. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    Published
    Aug. 20, 2024, 1:15 p.m.
    ...picked up by the sensors. You can adjust this by changing the voltage for the particular fluorophore prior...protein into your cells (visualized via the FITC channel) and you are trying to see what percentage of total...expression, using a histogram plot and MFI values to characterize your samples may allow for better interpretation...

  19. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    Published
    May 25, 2017, 2:30 p.m.
    ...imaging! These eNL constructs use the brightest characterized luciferase, NanoLuc, and its substrate furimazine...Nagai laboratory has a done a wonderful job characterizing Nano-lanterns and eNL for many different applications...eNL-based calcium sensor displaying a 500% signal change upon calcium binding. Suzuki et al. also envision...

  20. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    Published
    Jan. 18, 2018, 2:34 p.m.
    ...IPTG, the lac repressor undergoes a conformational change that removes it from lacO sites within the promoter...there is very little crossover. The induction mechanism must also be compatible with your experimental...The Promoter Region Plasmids 101: Cre-lox Light-Switchable Two-Component Systems for E. coli Browse other...
Showing: 401 - 420 of 803 results