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Showing: 401 - 420 of 756 results

  1. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    Published
    Feb. 2, 2021, 2:15 p.m.
    ...companies across most industries tend to hire their former interns to fill entry-level positions right out...grammatical errors, sentence structures, and overall format (fonts, font size, legibility, and more). Tips ...just as you would for an in-person interview. Formulate and practice a set of common questions with a ...technology, background, and lighting for a smooth performance. Most importantly, make certain that everyone...

  2. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    Published
    March 23, 2017, 2:30 p.m.
    ...improving access to useful research materials and information. Addgene has been successful in helping thousands...Addgene providing plasmids in a ready-to-use viral format. Having identified this need in the community,...The viral vector project team develops methods, performs extensive quality control, generates  educational... and Finance Teams continue to facilitate the formation of partnerships to expand the service and figure...

  3. A Deep Dive into BioBuilder

    Type
    Blog Post
    Published
    Oct. 11, 2017, 1:44 p.m.
    ...cutting-edge research projects in synthetic biology and transforms them into modules that students and teachers ...Murray. Murray’s lab uses cellular extracts to perform transcription and translation in vitro, and the... mentors, and provides a nationally recognized platform in March to exhibit projects at any stage of completion...runoff from winter icing.BioBuilderClub is the only informal synthetic biology club of its kind, running from...

  4. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    Published
    Sept. 15, 2020, 1:15 p.m.
    ...however, can give you this information (more on this below). Plating the transformation on ampicillin plates... our blue-white screening blog post for more information.   Figure 1: Result of a blue-white screen...Sometimes common  primers can't provide all of the information you need, so you'll want to design a custom primer...

  5. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

    Type
    Blog Post
    Published
    Feb. 24, 2015, 7:50 p.m.
    ...backbone, transformations require electroporation. References & Further Reading: For more information about...genome-wide library to activate all 23,430 known coding isoforms from the RefSeq database. The key to developing...crystal structure. “I thought maybe we could use information from the structure to guide and improve activator...

  7. dTAG - You're it!

    Type
    Blog Post
    Published
    June 21, 2018, 2:06 p.m.
    ...proteins. The dTAG molecule induces ternary complex formation between the FKBP12F36V-fusion and the ubiquitin...Chemical Biology additionally provides detailed information on cloning procedures used to construct the deposited...dTAG-13 due its immediate action, potent ligand performance, great depth of degradation, absolute selectivity...FKBP12F36V-tagged proteins using our lentiviral expression platform, to assess selective degradation of FKBP12F36V-BRD4...

  8. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    Published
    Nov. 3, 2020, 2:15 p.m.
    ... forces scientists to use invasive measures to perform in vivo optogenetic experiments. Intracranial injections... this library were designed using structural information about parental channelrhodopsins to create recombinant...correlated with the model’s predictions.  Three top performers, named ChRger1, ChRger2, and ChRger3, were then...minimally-invasive optogenetics While ChRgers performed well in a tissue culture dish, that doesn’t guarantee...

  9. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    Published
    April 12, 2016, 2:30 p.m.
    .... CombiGEM-CRISPR: The marriage of two simple platforms CRISPR has revolutionized how we decode the genome...regulation in biological systems. The CombiGEM platform provides a means to create barcoded gRNA libraries...library sequences via oligo synthesis using the format indicated below and pool-clone them into the pAWp28...Wong et al., 2016). With CombiGEM and CRISPR platforms now being integrated, we look forward to the realization...

  10. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ...then return information such as nutritional status. Within the animal kingdom, this information is relayed...datasets and reagents in the field. Additional information can be found in the Datasets and Reagents sections... The development of the transient transfection platform for assaying transcriptional activity, for example...

  11. How to Prepare for an Industry Interview

    Type
    Blog Post
    Published
    July 9, 2020, 1:15 p.m.
    ...transitioned between different lab groups without having a formal interview process. Industry interviews, however...general questions about job responsibilities, performance evaluations, professional development, and company...ask the hiring manager or recruiter for this information. I found it useful to write my list of questions...the hiring manager or recruiter what presentation format they require. Frequently they will ask you to present...

  12. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    Published
    Oct. 22, 2014, 1:57 p.m.
    ...updates. Panel discussions are becoming a ubiquitous format for events and sessions in scientific conferences...good panel discussion is a well choreographed performance with room for fortuitous improvisation. You want... have the correct date, location and parking information and remind them about the event as it approaches... put your panelists behind a table. It adds a formality and distance that prevents interaction with each...

  13. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ... need to deliver the Cas protein altogether. Platforming AAV Interest in interrogating and mapping the...glioblastoma, and generate programmable screening platforms for mouse tissues. Your AAVancement could be next...highly scalable cell type-specificin vivoscreening platform. bioRxiv (Cold Spring Harbor Laboratory). https...CRISPR Delivery Genome-wide Screening Using CRISPR Performing In Vivo CRISPR Screens Using the FITS Approach...

  14. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type
    Blog Post
    Published
    Dec. 12, 2013, 3:35 p.m.
    ...important to understand that these platforms, or pieces of a platform, that are now available exist within...’s only the promises and associated sequence information. Addgene has partnered with the BioBricks Foundation... good ways of converting typically invisible information into actionable signals. Let's say I wanted to...

  15. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...have advantages: they are convenient and provide uniform, consistent results. But they are also less accessible...remove RNA contamination and you can use a phenol chloroform extraction to remove remaining proteins and RNase...when the kits buffers ran out. Homemade buffers performed the same as those provided with the kit. Although...the results of transfection assays. Go forth and perform your DNA extractions without kits! Regardless of...

  16. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type
    Blog Post
    Published
    Sept. 23, 2014, 3:05 p.m.
    ... up after trying out the new protocols.io beta platform for sharing, annotating, and storing life science...associated protocols. When you upload your plasmid information, you’ll have the option to add additional documents...restriction digest of plasmid DNA, and bacterial transformation. And more plasmid-related educational resources...easy for you to begin using. For the novice, the format does have a learning curve, but OWW walks you through...

  17. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...all over the world looking for some additional information or clarification on these vectors. Read on to... for packaging (that's gag, pol, and env), the isoforms of these proteins, as well as the viral long terminal...shown to repress expression of HIV-1 LTRs (more information is available here). 2nd vs. 3rd Generation Lentiviral...with respect to the specific experiments being performed and you should understand the guidelines for using...

  18. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    Published
    May 21, 2024, 1:30 p.m.
    ...develop a successful, reproducible assay. That information can make you a much stronger candidate!  Additional...via their papers and/or website and use that information to explain why you are interested in working ...of a cover letter Cover letters follow the same format regardless of whether you’re in academia, industry... preparation for updating this post, I took an informal poll of Addgene hiring managers. All of them stated...

  19. How to Be an Excellent Trainee

    Type
    Blog Post
    Published
    Sept. 26, 2023, 1:15 p.m.
    ...protocol(s). Is there enough information there for you to be independent performing the technique you trained...benefits: you may learn faster and retain more information plus your mentor will get you up to speed easier...you need from them, and you can understand the information in your training. Bench mentor-mentee relationships...

  20. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ...the BP and/or LR reactions are performed, the next step is to transform competent E. coli cells and select...our recent Plasmids 101 Post on CcdB for more information. How to clone using Gateway technology To better...sequencing or restriction digest! Then, you can transform or transfect the cells that you want to use for...
Showing: 401 - 420 of 756 results