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We narrowed to 833 results for: ins

Showing: 401 - 420 of 833 results

  1. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ..., and Cas11. Type I-E Cascades contains all five of these Cas proteins, while type I-C Cascades do not...boundaries defined by anti-CRISPR proteins or running into other proteins naturally bound to the DNA. Cas3...The helicase domain is then activated, and Cas3 begins unraveling the double-stranded DNA (dsDNA) in what...Cas9 vs. The Other Cas(s) CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems INTEGRATE: Bacterial...

  2. Common Injection Routes in Mice

    Type
    Blog Post
    Published
    Jan. 22, 2025, 9:42 p.m.
    ... as “instillation” or “gavage,” as they do not require any punctures. Intratracheal instillation Intratracheal...Skill required: advanced Intranasal instillation Intranasal instillation is very similar to its intratracheal...compounds, as circulation is slower, and more institutional oversight is required. Restraint: mechanical...Intratracheal instillation is used to deliver substances directly to the lungs. It is an advanced technique...

  3. Antibodies 101: Flow Compensation

    Type
    Blog Post
    Published
    May 16, 2024, 1:15 p.m.
    ... depending on your flow cytometer’s setup. For instance, BV510 and AmCyan have almost identical emission...and how do I fix it? When plotting two channels against each other, you should be able to draw perpendicular...single-color APC control, with APC and APC-CY7 plotted against each other. Since it is a single-color control,...After increasing the compensation values for APC against APC-Cy7, the populations are in more of a straight...compensation value of five was applied to APC-Cy7 against APC to correct for this bleed. A compensation value...population is not a double-positive population and is instead due to bleeding, you will see the populations move...

  4. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    Published
    May 22, 2015, 4:58 p.m.
    ...Wyss Technology Development Fellow at the Wyss Institute and Harvard Medical School. Scientists making ...drive? The simplest way to make a gene drive is to insert an endonuclease gene within its own cut site1. ...spreads through the wild population and immunizes it against the first drive. Laboratories interested in building...question), why not use them whenever applicable?Against this, consider the cost of an accidental release...accidental release could delay real-world applications against scourges such as malaria and dengue for many years... Holm, D. G. & Suzuki, D. T. Genetic Control of Insect Populations: I. Cage Studies of Chromosome Replacement...

  5. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...causes the proteins and genomic DNA to precipitate. Plasmid DNA is free in solution. Proteins and genomic...the same principles: cells are lysed, RNAs and proteins are removed to leave you with DNA. This DNA is...you lyse the bacteria and denature the DNA and proteins in solution. The pH is then lowered using a renaturing...phenol chloroform extraction to remove remaining proteins and RNase. Finally, DNA is precipitated with alcohol...identical to that of commercially available kits but instead of binding DNA to silica columns, it’s bound to...

  6. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    Published
    Sept. 6, 2018, 12:02 p.m.
    ...such as PX459, which encodes Cas9 and contains a site for inserting a custom gRNA.  While this methodology...translation delays editing What are Cas9-gRNA ribonucleoproteins? One alternative approach, which avoids many...Schier laboratories. Delivering Cas9-gRNA ribonucleoproteins  An advantage of performing CRISPR experiments...vivo applications. This technique utilizes Cas9 proteins harboring receptor ligands (C in the figure above...., et al. "Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing...

  7. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    Published
    Oct. 19, 2017, 1:54 p.m.
    ...expression while proteins needed for later experiments (cellular tracking for instance) can be controlled...Tamoxifen is added. Tamoxifen then binds to the ER-LDB domains of the fusions allowing Cre/FLP translocate to ...fluorescent cytoplasmic markers (e.g. eYFP) and synaptic proteins (e.g. postsynaptic PSD95-mcherry) are used in ...In order to analyze the function of individual proteins in neuronal circuits or synaptic remodeling, researchers...is fascinated by the question of how genes and proteins coordinate billions of neurons to form the functional...

  8. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    Published
    July 25, 2019, 12:03 p.m.
    ...these experiments can be done in days to weeks instead of months to years. In the plant sciences “transient...evaluate its function. In nature Agrobacterium contains a plasmid with a T-DNA (transfer-DNA) region. ...pSB166 - pL2_pSB90_tMAS::rLUC-I::pMAS. Fluorescent proteins (FP, e.g. GFP) can be used in many ways. Often...from Agrobacterium. Test different Agrobacterium strains We used the disarmed Agrobacterium tumefaciens ...plants using syringe agroinfiltration." Recombinant proteins from plants. Humana Press, 2009. 41-50. PubMed... 5.02 (2003): 103-115. Hu, Ching-yeh, et al. "Intrinsic GUS-like activities in seed plants." Plant cell...

  9. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ...yourself needing to brainstorm with a team. One major challenge with these brainstorming sessions is avoiding...with unlimited time? Another strategy for your brainstorm session is to start with something completely... students during an interview. When they were instructed to use the three-ring binder as a starting point...people to agree with the most prominent idea, and instead building an environment where everyone has their... ideas that I have found helpful in my team brainstorming sessions.  Allow the group time to think about...

  10. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    Published
    May 20, 2025, 1:15 p.m.
    ...origin of replication (ori), where DNA replication begins, so a bacterial plasmid needs a compatible ori ... divide. In transient transfection, the vector remains episomal, meaning it stays separate from the genome...synthesis, and the neomycin resistance gene protects against any of them. But different aminoglycosides work...options doesn’t end there. Make sure you read the instructions for whatever antibiotic you choose to ensure...to spontaneously develop resistance to selection toxins. Auxotrophic selection is useful for producing ...researcher may choose not impose selection on cells and instead to use a vector with a reporter, such as a fluorescent...

  11. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ...with higher specificity (eSpCas9 and HypaCas9) instead.  Choosing a Cas9 for site-specific modifications... effects. The SpG variant, discovered by the Kleinstiver group has a PAM of NGN (Walton et al., 2020)....system can be used with a variety of impaired Cas9 proteins (such as SpRY) with altered PAM requirements, ...facilitates recruitment of multiple activation domains to the same genetic locus via co-expression of ...epitope tagged dCas9 and antibody-activator fusion proteins. The Synergistic Activation Mediator complex, ..., R. T., Christie, K. A., Whittaker, M. N. & Kleinstiver, B. P. Unconstrained genome targeting with near-PAMless...

  12. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    Published
    Oct. 13, 2016, 2:30 p.m.
    ...pharmaceutical companies, biotech start-ups, health insurance companies, and governments on the most effective...physicians and patients, shaping discussions with insurance companies about the pricing and coverage of drugs...situations might be especially challenging. For instance, the client might need to get a large amount of...occasions, clients can be excessively demanding. For instance, a client may offer a lot of pointed feedback ...understanding of the molecular behaviors of chemicals and proteins to learn how a particular drug or medical device...therapeutic approach brings to patients, doctors, and insurance providers, and is crucial to how our clients’ ...

  13. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ...unstable in vivo due to poor folding and thermal instability at 37°C (Ouellet et al., 2016) and requires a...riboswitch, metabolite binding to the riboswitch instead activates Spinach fluorescence. In the search for...identified the nucleotides that contribute to the instability of Spinach, and were able to develop an aptamer...Spinach, a miniaturized version of Spinach that retains similar levels of fluorescence when bound to DFHBI... cell imaging. Bunch of baby spinach, or BoBS, contains tandem repeats of Baby Spinach. It was developed... Aliyah Weinstein...

  14. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ..., R., Weinstock, G., Metzker, M. et al. Genome sequence of the Brown Norway rat yields insights into mammalian...organism in microbiology and biochemistry. Certain strains have been specifically developed to thrive in laboratory...extensive research history and characterization. Many proteins and processes naturally found in E. coli have ...amphibians in science with early research providing insight into some of the most significant and fundamental...), 565–571. https://doi.org/10.1159/000323761 Modlinska, K., & Pisula, W. (2020). The Norway rat, from...

  15. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...to reveal even more insights. Super-duper lipid editor And if recruiting proteins to the cell membrane...photoswitchable elements into many other types of proteins. Here, we’ll highlight a few recently-deposited...et al., 2022). Stopping traffic with CIB1-Rabs Instead of using an optogenetic module to activate or recruit...such a strategy to dissect the roles of three Rab proteins during a key stage of zebrafish development (Aljiboury...(Aljiboury et al., 2023). Rab family proteins support vesicle trafficking and organelle dynamics, but ...

  16. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    Published
    Aug. 2, 2016, 2:30 p.m.
    ...reporter constructs), in functional analysis of proteins (to perform alanine scanning mutagenesis or targeted...should be resistant to Cas9 cleavage. If a plasmid contains the template, site-directed mutagenesis can be...which is dam+). Methylation deficient bacterial strains can be identified by the dam13(-) mutation – you...you’ll want to steer clear of these strains when preparing the plasmid template for site directed mutagenesis...functional regions of the plasmid including the insert. This is essential since mutations are potentially...PubMed PMID: 2448875. 4. Xia, Yongzhen, et al. "New insights into the QuikChangeTM process guide the use of...

  17. Evolution of Lab Techniques

    Type
    Blog Post
    Published
    June 21, 2016, 2:30 p.m.
    ...whether you are perusing classic papers or are brainstorming new technological innovations. Let’s take a ...blotting to screen founder transgenic animals for the inserted transgene because Southern blots have a lower ...digests. A researcher can test the orientation of an insert in only a few hours at low cost with restriction...do not typically rely on Sanger sequencing but instead use methods like pyrosequencing that are parallelized...techniques we use now may soon become obsolete. For instance, the popular genome-engineering tool, CRISPR, ...mouse lines, such as BAC transgenic or traditional knockins/knockouts, a thing of the past. In the sequencing...

  18. Ghanaian Researchers to Receive Plasmid Awards from Addgene and Seeding Labs

    Type
    Blog Post
    Published
    June 15, 2016, 1:30 p.m.
    ...bioinformatic and proteomic tools to discover genes and proteins associated with pathogenesis. In his future work... T7 polymerase, EcoRI, and various fluorescent proteins. Dr. Duedu’s plans for these plasmids have a heavy...PCR. We hope these plasmids will help Dr. Duedu inspire new Ghanaian scientists to continue to do excellent...have received equipment through Seeding Labs’ Instrumental Access program. We are excited to discover what...Apply to Recieve Equipment through Seeding Labs' Instrumental Access Program Additional Resources on the ...

  19. A Scientist's Guide to Ebola

    Type
    Blog Post
    Published
    Oct. 16, 2014, 3:01 p.m.
    ...does all of its damage with just seven structural proteins. But, as NPR explained, it’s not the virus itself... critical for viral attachment and fusion. Once inside a host cell, new evidence in PLoS Pathogens shows...shows that the Ebola virus glycoprotein remains essential in allowing those infected cells to go undetected...in a novel way, by shielding host cell surface proteins. Researchers at the University of Virginia School...California, Santa Cruz, offers sequence data on five strains of Ebola representing the latest outbreak and earlier...

  20. How Exercise Purges Stress and Keeps Depression at Bay

    Type
    Blog Post
    Published
    Nov. 25, 2014, 4:02 p.m.
    ...researchers led by Dr. Jorge Ruas of the Karolinska Institutet, the discovery really wasn’t what they’d...those mild stressors as usual, but somehow their brains were protected. Effects of Activation of the PGC... Moon and Henriette van Praag of the National Institute on Aging in a commentary about the new work published...explore. “It will be important to identify the key proteins and metabolites that are dynamically secreted ...
Showing: 401 - 420 of 833 results