...2.1, GFP is tagged with a nuclear localization sequence, allowing it to localize to a different area of...

...interest, flanked by long terminal repeat (LTR) sequences, which facilitate integration of this portion ...considered a double-edged sword with unintended consequences. In this blog, we’ll take a deep dive into integrase-deficient..., & Ciuffi, A. (2012). Viral Integration and Consequences on Host Gene Expression. Viruses: Essential ...

...analyze your samples, it is necessary to set up a sequence of gates to be able to select and precisely measure...

...ll, of course, mention Addgene as a source of sequenced and quality controlled plasmids and viral vectors...

...Denature the samples to their primary amino acid sequence by boiling in the presence of a reducing agent...

...gene is very large (79 exons), but much of the sequence appears to be nonessential. Within the mutational...

...are too long for rAAVs, which can only package sequences shorter than 5 kb (Hirsch et al., 2016; Hudry ...

...cell therapeutics because they can help start a sequence of immune reactions that aid in the fight against...

...pluripotent stem cells free of vector and transgene sequences. Science, 2009. 324(5928): p. 797-801. PubMed ...

...therefore necessitates careful design of RNAi sequences, confirmation of RNA knockdown by RT-qPCR, and...

... In addition, rAAV2-retro’s altered amino acid sequence might facilitate interaction with uptake or intracellular...

...brief window in time “The most obvious future consequence of the undermining of the scientific process ...still is and will continue to be a “critical consequence of ...the marginalization of science,” we know...know now in the summer of 2020 that the consequences of undermining science are even more immediate and...

...the quantity of DNA that is required and, as a consequence, also dictate the required sizes of the bacterial...micrograms of plasmid DNA for each reaction. As a consequence, the preparations for this technique must be ...

...chosen to leave the lab and march. Unintended consequences While frustration and anxiety have inspired ...others are concerned about possible unintended consequences of a march. Three of these risks include: 1)...

...dissociate due to conformational changes, and as a consequence, EB1 protein function is inactivated by the separation...

...current job as a Marie Curie Fellow is a direct consequence of a blog that I started writing three years ...

...and decreased functionality are all possible consequences of FP aggregation, as Erik Snapp’s blog post...

...underutilized in research settings — mostly a consequence of Cascade's complexity. Figure 1: Schematic...

...that two FPs will collide with each other. The consequences can be dramatic. For a membrane POI localized...

...incorrect assumptions can be a direct cause or consequence of lapsed communication. Making some assumptions...