We narrowed to 430 results for: sequence

1. An Introduction to Adenovirus

   Type

   Blog Post

   Published

   Oct. 3, 2023, 1:15 p.m.

   ...genomic plasmid can be used to house a recombinant sequence (transgene) instead.    Although the late genes...

2. Plasmids 101: Broad Host Range Plasmids

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   Blog Post

   Published

   April 11, 2024, 1:15 p.m.

   ...initiation proteins. These iterons vary in their sequence and number and can alter the efficiency of replication...

3. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

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   Blog Post

   Published

   Sept. 13, 2018, 1:55 p.m.

   ...2) non-targeting guides (red), which target no sequence in the genome, and are commonly used as negative...

4. Rett Syndrome: A History of Research and Therapeutic Outlooks

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   Blog Post

   Published

   Nov. 19, 2020, 2:15 p.m.

   ...interacts with the minor groove of AT-rich DNA sequences to regulate transcription (Baker et al., 2013)...

5. The Fluorescent Vegetables in Aptamer Soup

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   Blog Post

   Published

   Jan. 29, 2019, 2:27 p.m.

   ...processes including RNA movement by tagging RNA sequences with an aptamer and RNA transcription by placing...

6. How to Negotiate a Successful Lab Start Up Budget

   Type

   Blog Post

   Published

   Sept. 1, 2015, 2:30 p.m.

   .... Major equipment includes large centrifuges, sequencers and analyzers, and any ultra sensitive equipment...

7. Viral Vectors 101: Systemic Capsids

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   Blog Post

   Published

   Oct. 12, 2023, 1:15 p.m.

   ... miRNA target sites, and recombination target sequences, denoted by triangles] facilitate tissue or cell...AAV-Mediated Transcriptional Changes via Single-Cell RNA Sequencing. Frontiers in immunology, 12, 730825. https:/...N., & Pfenning, A. R. (2022). Machine learning sequence prioritization for cell type-specific enhancer...

8. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

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   Blog Post

   Published

   May 13, 2025, 1:15 p.m.

   ...interest, flanked by long terminal repeat (LTR) sequences, which facilitate integration of this portion ...considered a double-edged sword with unintended consequences. In this blog, we’ll take a deep dive into integrase-deficient..., & Ciuffi, A. (2012). Viral Integration and Consequences on Host Gene Expression. Viruses: Essential ...

9. Antibodies 101: Introduction to Gating in Flow Cytometry

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   Blog Post

   Published

   April 4, 2024, 1:15 p.m.

   ...analyze your samples, it is necessary to set up a sequence of gates to be able to select and precisely measure...

10. Harnessing Bacterial Toxins for Allelic Exchange

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    Blog Post

    Published

    Aug. 15, 2019, 12:30 p.m.

    ...computer program for the construction of polylinker sequences of molecular vectors". Proceedings of the Belarusian...

11. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type

    Blog Post

    Published

    March 5, 2019, 1:08 p.m.

    ...2.1, GFP is tagged with a nuclear localization sequence, allowing it to localize to a different area of...

12. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type

    Blog Post

    Published

    May 18, 2018, 7:32 p.m.

    ...ll, of course, mention Addgene as a source of sequenced and quality controlled plasmids and viral vectors...

13. Antibodies 101: The Basics of Western Blotting

    Type

    Blog Post

    Published

    March 8, 2021, 2:41 p.m.

    ...Denature the samples to their primary amino acid sequence by boiling in the presence of a reducing agent...

14. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type

    Blog Post

    Published

    Jan. 26, 2016, 3:30 p.m.

    ...gene is very large (79 exons), but much of the sequence appears to be nonessential. Within the mutational...

15. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type

    Blog Post

    Published

    Oct. 17, 2019, 12:28 p.m.

    ...are too long for rAAVs, which can only package sequences shorter than 5 kb (Hirsch et al., 2016; Hudry ...

16. Cancer and the Immune System: Deciphering the Relationship

    Type

    Blog Post

    Published

    March 14, 2017, 2:30 p.m.

    ...cell therapeutics because they can help start a sequence of immune reactions that aid in the fight against...

17. Antibody Validation for Flow Cytometry

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    Blog Post

    Published

    Sept. 10, 2024, 1:15 p.m.

    ...therefore necessitates careful design of RNAi sequences, confirmation of RNA knockdown by RT-qPCR, and...

18. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type

    Blog Post

    Published

    Nov. 6, 2018, 1:12 p.m.

    ...pluripotent stem cells free of vector and transgene sequences. Science, 2009. 324(5928): p. 797-801. PubMed ...

19. Using AAV for Neuronal Tracing

    Type

    Blog Post

    Published

    Aug. 9, 2018, 1:04 p.m.

    ... In addition, rAAV2-retro’s altered amino acid sequence might facilitate interaction with uptake or intracellular...

20. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type

    Blog Post

    Published

    July 30, 2020, 1:15 p.m.

    ...brief window in time “The most obvious future consequence of the undermining of the scientific process ...still is and will continue to be a “critical consequence of ...the marginalization of science,” we know...know now in the summer of 2020 that the consequences of undermining science are even more immediate and...