We narrowed to 711 results for: tat
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TypeBlog PostPublishedJune 16, 2020, 1:25 p.m....important to consider your experiment. It will dictate the amount of DNA you need, and at which level ...DNA that is required and, as a consequence, also dictate the required sizes of the bacterial cultures and...
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Making Career Development an Integral Part of the Science Training System
TypeBlog PostPublishedFeb. 25, 2021, 2:15 p.m....Steering Committee and working groups with representatives from all areas of the scientific training and...Sciences (NIGMS) will support the development and implementation of collections of evidence-based practices.... -
More Data for You: Find Articles Citing Addgene Plasmids
TypeBlog PostPublishedJune 16, 2015, 2:15 p.m....then you'll find the plasmids listed below the citation. An example of these "Associated Plasmids" is ...increasing your work's visibility Help us improve our citations lists We are still growing our "Articles Citing... -
A Conference By Postdocs For Postdocs: Future of Research
TypeBlog PostPublishedSept. 2, 2014, 3:05 p.m.... in some form? There is a lot of talk about the state of scientist training in the US and around the world...postdoc-driven biotechnology clubs such as Penn State, Berkeley and Harvard. Students also partner with... -
Science Communication for Everyone
TypeBlog PostPublishedApril 16, 2019, 1:05 p.m....sci-art that would ultimately give a visual representation of science to our audience. How can researchers...extensively use Google search. Be careful about hidden connotations behind a word. Non-scientists interpret terms... -
CRISPR-Cas14: a family of small DNA-targeting enzymes enabling high-fidelity SNP genotyping
TypeBlog PostPublishedNov. 29, 2018, 1:53 p.m....CRISPR array and intergenic regions containing the putative tracrRNA into a plasmid and expressed it in an...infectious organisms (ex: SARS-CoV-2) and genetic mutations. By providing a gRNA specific of a viral sequences... -
Measuring Kinase Activity at the Single-Cell Level with Kinase Translocation Reporters (KTRs)
TypeBlog PostPublishedJuly 26, 2018, 12:46 p.m....activity depends on both the distance and the orientation of the donor and acceptor proteins, which can...of the nucleus depending on its phosphorylation status. The relative ratio of cytoplasmic vs. nuclear ... -
Fluorescent Proteins 101: History of Fluorescent Proteins
TypeBlog PostPublishedAug. 7, 2017, 1:58 p.m....the Pacific Ocean, off of the coast of Washington state. Using these samples, he was able to isolate two... protein’s structure and also discovered many mutations that alter and improve its function and shift ... -
Addgene's Top Blog Posts from 2020
TypeBlog PostPublishedDec. 29, 2020, 2:15 p.m....this blog post. 9. Droplet Digital PCR for AAV Quantitation by Kate Harten DeMaio We made the switch to...blogger Shravanti Suresh who studies CRISPR at Iowa State University detailed five methods for using CRISPR... -
Developing Transferable Skills During Science Training
TypeBlog PostPublishedJune 3, 2014, 2:14 p.m....you need to, go to ESL classes Practice your presentation skills (as much as you can), form a peer mentoring...content-useful website, write a blog Pursue speaking invitations by inviting others (be on a speaker committee... -
Why Do I Need an MTA Anyway?
TypeBlog PostPublishedMay 1, 2014, 8:23 p.m....pride in facilitating the exchange of not just the physical samples, but also the legal documentation that... -
Let There Be LITE Plasmids
TypeBlog PostPublishedNov. 21, 2013, 3:56 p.m....players in normal biological processes and disease states. While LITE plasmids have clear application to ...mammalian endogenous transcription and epigenetic states." Nature. 500, 472–476 (22 August 2013). , ... -
Editor's Choice, September 2016
TypeBlog PostPublishedOct. 7, 2016, 4 p.m....guest blogger Sean Stacey from Eastern Tennessee State University and provides a brief rundown of Sean’...phage are super cool and have considered getting a tattoo of my favorite molecular biology tool, P1 phage... -
Editor's Choice, August 2016
TypeBlog PostPublishedSept. 2, 2016, 1:32 p.m....personal experience, is a quick way to generate many mutated plasmid constructs. The second post was contributed...specificity issues, and other means of generating mutations in live cells are all discussed further in this... -
Management for Scientists: Seeking Feedback
TypeBlog PostPublishedJan. 8, 2015, 2:34 p.m..... Check out Joanne's Reddit AMA Here they are annotated with my comments: Everyone on the team talks...complaining. They may worry that it seems like "tattling" if others are involved. They may feel they are... -
IDP and your PI: A Roadmap for Career Planning and Personal Development
TypeBlog PostPublishedJuly 21, 2015, 2:30 p.m....help each person organize his or her thoughts to facilitate honest conversation. Goals and Planning Worksheet...effort needed to complete them. These documents dictate the subject matter discussed at the meeting, but... -
CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]
TypeBlog PostPublishedFeb. 18, 2015, 3:09 p.m....sequences often result in loss-of-function frameshift mutations. However these may require restriction fragment...allele is likely to bear an indel or inversion mutation [3]. In the CRISPR era, the genomic landscape ... -
What's New in CRISPR - September 2019
TypeBlog PostPublishedSept. 10, 2019, 12:56 p.m.... Using the structure of CasX at eight different states of assembly from cryo-electron microscopy, the ...activation and genome editing by fusing Cas9 to a mutated human estrogen receptor. This protein fusion stays... -
Working 9-5: an Addgene Co-op
TypeBlog PostPublishedJune 2, 2022, 2:15 p.m....wanted to ensure applicants understood what the expectations were for the intended position. After submitting...Addgene 101” series, where each team gives a presentation on what they do and how it relates to other ... -
New Norepinephrine Indicators: nLightG and nLightR
TypeBlog PostPublishedDec. 12, 2023, 2:15 p.m..... The Patriarchi Lab aimed to overcome these limitations by developing and depositing two new norepinephrine... using ColabFold (Mirdita et al., 2022). B, Representative images of HEK293T cells and neurons expressing...