...cell type that can differentiate into hepatic or intestinal cell types. MEFs were reprogrammed into iEPs ...least day 13. However, distinct transcriptional states were seen as early as day 6. All together these...in a competitive transplant assay in the mouse intestine, demonstrating its utility in long-term tracking...

...shown that cells treated with sucrose or other osmolytes to induce hypertonicity display enhanced monoclonal...impaired endocytosis. In parallel assays designed to test the endocytosis of a fluorescent dye and transduction...host of other AAV serotypes; it will be exciting to test this approach with different capsids. Most importantly...

...Avidin and its derivatives have four biotin-binding sites per molecule so, theoretically, saturated avidin...were unable to cleave TEV within the protein we tested, it may be possible with other fusion proteins ...bacteria significantly enhance protein folding (tested using real science). Make a stock solution of 2.5...

...building that someone from a different company had tested positive for COVID-19 (it turned out to be an unfounded...operations until our normal work resumed. Addgene distributes and fulfills requests for scientists doing almost...type of biomedical science research. Our work accelerates and enhances productive collaborations as well...

...effective substrates for tagmentation-based library preparation procedures. This finding eliminates the double-stranding...be run in parallel and provide serotype data in minutes and the scripts  can be easily customized to detect...

...avoid off target photostimulation of axons and dendrites on neighboring neurons. We have also packaged ... AAV in smaller aliquots of 20 ul for serotype testing to help you pick the best AAV for your experiment...control plasmids in new serotypes. See our Serotype Testing AAV inventory for the entire collection! Plasmid...

...scicomm group project on the Leith river. Photo courtesy of Leonardo Ramos.  I enrolled into a summer...industry. Along this road, science communication graduates are often well-equipped. Many institutions are...explores risk-taking behavior among collision sport athletes, as well as the ethical and health policy implications...

...have recently received a grant from the National Institutes of Health that will address this problem.The ...animals may be variable among animals and bleed dates. The development of monoclonal antibody (mAb) technology... represent the official views of the National Institutes of Health.     References and Resources References...

...is mind-boggling. Unlike a mountain – with many routes up and only one destination – a career in the sciences...molecular pathways, I found myself curious about other routes – In terms of both types and topics of work. A ...rare organisms so I found it hard to stick to vertebrates alone.  And a longstanding inclination to be ...

...physical and chemical conditions like YNP thermal sites - are also of practical interest since they’ve previously...distance. At each collection site, we took GPS coordinates, water temperature readings using a heat gun,...the van der Oost lab. Phylogenetic comparison indicates that like GeoCas9 and ThermoCas9, IgnaviCas9 is...

...of heredity were thought of as everything from parasites, to symbionts, to genes and the labels applied... the National Academy of Sciences of the United States of America 110.39 (2013): 15521–15529. PMC. PubMed... the National Academy of Sciences of the United States of America 70.11 (1973): 3240–3244. PubMed PMID...

... to read a little more this October. Science favorites Musicophilia: Tales of Music and the Brain by ...Communications and Marketing Coordinator Non-science favorites  The Mistborn series by Brandon Sanderson "The... reader of historical fiction (I can personally attest to this!). Following history from the Ice Age to...

...the selected card on the board game. They had 35 minutes to develop a strategy and build a transfer plasmid... manipulate human stem cells. Then they had 10 minutes to expose their strategy, justifying each single...lentivirus-based ex-vivo gene therapy’ strategies to undergraduates, however you may use it and modify it for non-commercial...

...variety of research applications and particularly notes their ability to be precisely spatio-temporally ...down the HMO of interest, the lactose released activates expression of GFP from the lac operon. GFP expression...Light to Control CRISPR Genome Editing Choose the Brightest Fluorescent Protein for Your Experiments Resources...

...measures these absorbances using UV-transparent cuvettes. First you measure the absorbance of the buffer...-2 ul of sample and the run time is just a few minutes per sample. During the run, DNA fragments move ...quantified over time using a fluorescent dye that intercalates into the DNA such that smaller fragments are...

...stood out from their initial testing. It outperformed other tRNA candidates (either from human or plant...muscular dystrophies, heart disease, and type 2 diabetes, across four different types of human cells, including...

...there is someone you personally know or your lab collaborates with that can fulfill your research needs, that... that is probably the easiest path. Some institutes have structured visiting positions within defined ... any protocols the day before, etc.), take good notes, and ask questions if you don’t understand something...

...specific subsets like CD4+ or CD8+ T-cells or monocytes. PBMCs can be further differentiated into dentritic...decisions about in vivo application and clinical testing. This would pave the way for improved patient outcomes...Burns-Naas, L. A. (2015). Chapter 27—Immunotoxicology Testing of Monoclonal Antibodies in Macaca fascicularis...

...area, as the image below shows:    This system ablates tumors just as effectively as standard CAR-T cells...elevated at stable levels for 6 days - after just 15 minutes of FUS.  Similar constructs to the one above can...conditions better than most FPs. The group also tested hfYFP in expansion microscopy as well as correlative...

...CRISPR/Cas system, Cas9 is guided to its target sites with the aid of two RNAs: the crRNA which defines... as a scaffold linking the crRNA to Cas9 and facilitates processing of mature crRNAs from pre-crRNAs derived...modify the desired genomic locus.   Some historical notes CRISPR arrays in bacterial genomes consist of repeated...