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We narrowed to 707 results for: ust

Showing: 401 - 420 of 707 results
  1. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ...targeting Cas enzymes Move over Cas9, CRISPR isn’t just for DNA anymore! Cas enzymes have been isolated ...traditional spCas9. If you are looking to modify RNA, Cas9 just won’t cut it – literally. Cas13 Cas13 is an RNA-guided...
  2. Developing Lab Management Software for Biology

    Type
    Blog Post
    Published
    Aug. 25, 2016, 2:30 p.m.
    .... The benefits of lab management software aren’t just limited to large volume facilities; it can be useful...ensure that everything is grown successfully, we must track and manage all of these steps. Unfortunately...
  3. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ...software such as Primer3 to help you design your custom primers. Remember, when designing a sequencing ...outlined above. Avoid primers that bind within or must read through repetitive regions (such as LTRs or...
  4. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    Published
    Oct. 8, 2024, 1:15 p.m.
    ...scientists use to promote transformation, but it isn’t just for lab specimens — there is evidence that lightning... helper viruses in AAV production systems. We’re just skimming the surface of the natural plasmid world...
  5. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    Published
    Sept. 27, 2017, 1:23 p.m.
    ...probably write for your institute’s blog. The Wellcome Trust blog or the John Innes Student Voice are two examples...creative – Bring your knacks into play Perhaps writing just isn’t your cup of tea, and you are more of an audio...
  6. Rabies and Neuronal Tracing

    Type
    Blog Post
    Published
    May 29, 2018, 1:51 p.m.
    ... to cross a synapse from one cell to another, it must replicate. Modified Rabies Virus: Converting a transsynaptic... network connections in a useful way, but rather just labels the whole brain. To achieve labeling of only...
  7. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ... though its maturation is even faster. Published just a few days later, Mohan Balasubramanian and colleagues...vascular bias in mature marmosets. CAP-Mac also robustly expressed fluorescent reporters in green monkeys...
  8. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...outstanding, with almost all patients experiencing sustained and substantial relief from both disorders. As...either clinically or financially), and others we just don’t have the time to talk about. Other treatments...
  9. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ...receptors are activated through administered drugs that must diffuse throughout the body, the onset of receptor... in the development of therapies when expression must be restricted to specific circuits. Methods for ...
  10. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    Published
    July 11, 2019, 1:26 p.m.
    ... in genetically identical mice. Thus, scientists must genotype each animal to identify the ones with the... variations of this technology can be used to customize the effects of this recombination. To generate...
  11. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    Published
    Oct. 22, 2019, 12:20 p.m.
    ... subtle gradient (as is shown in all of our illustrations above) so that the color fades to a lighter ...poster more accessible. Final thoughts Though we’ve just presented some standard formatting guidelines for...
  12. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    Published
    Aug. 30, 2016, 2:30 p.m.
    ...and submitted our manuscript to the journal Cell just six weeks later. The Zika publication timeline I...self-assembly for applications in health, energy, and sustainability.   References 1. Pardee, Keith, et al. "Rapid...
  13. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    Published
    March 31, 2016, 2:30 p.m.
    ...Plasmid DNA in both prokaryotic and eukaryotic systems must be transcribed into RNA, which occurs in three phases... Addgene.org Browse our Plasmid Reference Pages Just Starting Work in a New Field? Check out Our Plasmid...
  14. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ...like the HSV thymidine kinase (HSV-tk) is included just outside one of the homology arms. When we select...recombined cells. Recombination is a rare event, so you must select as opposed to screen for cells where recombination...
  15. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    Published
    Aug. 3, 2023, 1:15 p.m.
    ...the machine uses the full spectral signature, not just a narrow band, to discern one marker from another...Instead of compensation, which relies on user adjustment, spectral flow cytometry relies on “unmixing....
  16. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    Published
    Feb. 4, 2020, 2:15 p.m.
    ...biological research for a long time, chromoproteins are just now being developed for a broad range of biological...since you can see if your cloning experiment worked just by looking at the cells on the plate. For example...
Showing: 401 - 420 of 707 results