We narrowed to 694 results for: ust
-
TypeBlog PostPublishedSept. 27, 2017, 1:23 p.m....probably write for your institute’s blog. The Wellcome Trust blog or the John Innes Student Voice are two examples...creative – Bring your knacks into play Perhaps writing just isn’t your cup of tea, and you are more of an audio...
-
Hot Plasmids February 2024
TypeBlog PostPublishedFeb. 20, 2024, 2:45 p.m.... though its maturation is even faster. Published just a few days later, Mohan Balasubramanian and colleagues...vascular bias in mature marmosets. CAP-Mac also robustly expressed fluorescent reporters in green monkeys... -
Plasmids 101: The Wide World of Natural Plasmids
TypeBlog PostPublishedOct. 8, 2024, 1:15 p.m....scientists use to promote transformation, but it isn’t just for lab specimens — there is evidence that lightning... helper viruses in AAV production systems. We’re just skimming the surface of the natural plasmid world... -
Mouse Modeling, Part 1: Genetically Engineered Mice
TypeBlog PostPublishedJuly 11, 2019, 1:26 p.m.... in genetically identical mice. Thus, scientists must genotype each animal to identify the ones with the... variations of this technology can be used to customize the effects of this recombination. To generate... -
Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them
TypeBlog PostPublishedMay 4, 2021, 1:15 p.m....have a shelf full of CDs that have been gathering dust since the digital music revolution hit. I predict...functional antibody. These antibodies can be used just as you would use antibodies made from animals or... -
CRISPR in the Clinic
TypeBlog PostPublishedNov. 2, 2023, 1:15 p.m....outstanding, with almost all patients experiencing sustained and substantial relief from both disorders. As...either clinically or financially), and others we just don’t have the time to talk about. Other treatments... -
Viral Vectors 101: Chemogenetics
TypeBlog PostPublishedJuly 11, 2023, 1:15 p.m....receptors are activated through administered drugs that must diffuse throughout the body, the onset of receptor... in the development of therapies when expression must be restricted to specific circuits. Methods for ... -
A Guide to Designing a Scientific Poster: Content, Layout, and More
TypeBlog PostPublishedOct. 22, 2019, 12:20 p.m.... subtle gradient (as is shown in all of our illustrations above) so that the color fades to a lighter ...poster more accessible. Final thoughts Though we’ve just presented some standard formatting guidelines for... -
Rabies and Neuronal Tracing
TypeBlog PostPublishedMay 29, 2018, 1:51 p.m.... to cross a synapse from one cell to another, it must replicate. Modified Rabies Virus: Converting a transsynaptic... network connections in a useful way, but rather just labels the whole brain. To achieve labeling of only... -
Adapting Toehold Switches to Detect Zika Virus
TypeBlog PostPublishedAug. 30, 2016, 2:30 p.m....and submitted our manuscript to the journal Cell just six weeks later. The Zika publication timeline I...self-assembly for applications in health, energy, and sustainability. References 1. Pardee, Keith, et al. "Rapid... -
Plasmids 101: Terminators and PolyA signals
TypeBlog PostPublishedMarch 31, 2016, 2:30 p.m....Plasmid DNA in both prokaryotic and eukaryotic systems must be transcribed into RNA, which occurs in three phases... Addgene.org Browse our Plasmid Reference Pages Just Starting Work in a New Field? Check out Our Plasmid... -
Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles
TypeBlog PostPublishedJune 22, 2017, 2:30 p.m....ImageJ plugins JACoP and Coloc 2, BioImageXD, and customized CellProfiler pipelines. Image analysis software...select FPs for muli-color imaging. Furthermore, one must bear in mind that the resolution of light microscopy... -
Plasmids 101: Knockout/Knock-In Plasmids
TypeBlog PostPublishedDec. 1, 2016, 3:30 p.m....like the HSV thymidine kinase (HSV-tk) is included just outside one of the homology arms. When we select...recombined cells. Recombination is a rare event, so you must select as opposed to screen for cells where recombination... -
Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria
TypeBlog PostPublishedApril 4, 2019, 12:53 p.m....methods. First, its modular design allows for customization with strain-specific promoters, antibiotic resistance...species where its modular nature allows for the customization of guides, promoters, selection markers, and... -
CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments
TypeBlog PostPublishedFeb. 7, 2019, 2:16 p.m....eye. We came up with a ranking formula that does just this: Rank = (compressed CFD score) * (gaussian ...are transformed to 100. This transformation (illustrated in the graph below) is performed because weak... -
Antibodies 101: Conventional vs Spectral Flow Cytometry
TypeBlog PostPublishedAug. 3, 2023, 1:15 p.m....the machine uses the full spectral signature, not just a narrow band, to discern one marker from another...Instead of compensation, which relies on user adjustment, spectral flow cytometry relies on “unmixing.... -
Chromoproteins: Colorful Proteins For Molecular Biology Experiments
TypeBlog PostPublishedFeb. 4, 2020, 2:15 p.m....biological research for a long time, chromoproteins are just now being developed for a broad range of biological...since you can see if your cloning experiment worked just by looking at the cells on the plate. For example... -
Addgene's Cancer Collection Pages Connect the Research Community
TypeBlog PostPublishedSept. 6, 2016, 2:30 p.m...., Ras proteins have stumped the pharmaceutical industry for years. They have proven very difficult to ...be to follow up on these promising results and customize the small molecules for a higher affinity fit.... -
When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells
TypeBlog PostPublishedApril 19, 2016, 2:30 p.m....to be sold by Clontech and is in a freezer box in just about every lab in the world, is not inert. In all...problem with dimerizing fluorescent proteins is illustrated. 1) Membrane proteins fused to FPs that dimerize... -
The Importance of a Fun Workplace: Company Culture at Addgene
TypeBlog PostPublishedSept. 3, 2015, 2:30 p.m....Ladder Ball... there might be a possibility it was just beginner’s luck, but I’m feeling optimistic for ...activity into the mix It’s easy to sit around and just eat with your co-workers. Don’t fall into that rut...