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We narrowed to 790 results for: Cre

Showing: 211 - 220 of 790 results

  1. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...USA have used AAV1 and AAV2 serotypes in order to create an atlas of the mouse brain. This atlas enables...antibody recognition of epitope tags. Thus they have created antigenic protein tags called “spaghetti monster... FPs fused to multiple epitope tags. Indeed, to create the smFPs Looger’s team strategically inserted ...antibody-based amplification of their signals is weak and increases background fluorescence. Furthermore, many of ...instance, smFPs have been used to visualize “thorny excrescence” (TE) spines in the Hippocampal CA3 pyramidal...sites and primary antibody-based detection, smFPs create much brighter signals for weakly expressed proteins...: Making the journey from axon to nucleus AAVs CREATEd for gene delivery to the CNS and PNS Resources...

  2. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...Villarreal. By the late 1980s, scientists even created a codon adaptation index (CAI) that is based on... (Sharp and Li, 1987). In 2000 the Ikemura lab created the Codon Usage Tabulated from GenBank database...interest based on an organisms codon usage bias to increase translational efficiency and thus protein expression...across an entire polypeptide. Many researchers have created codon-optimization algorithms/resources (Athey ...study, codon optimization resulted in a 6-9 fold increase in expression of these channels providing scientists...to codon optimize? Although codon optimization increases protein production in certain systems, synonymous...optimization may not always be the optimal strategy for increasing protein production. If you are trying to express...

  3. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    Published
    Aug. 20, 2020, 2:30 p.m.
    ...s lab performed a series of positive selection screens in bacteria to identify mutants of S. pyogenes ...sequences (Kleinstiver et al. 2015). From these screens, they identified four novel SpCas9 variants with...some cleavage with an NGA PAM. This variant may increase the specificity of genome modifications at DNA...allows for expanded PAM recognition as well as increased specificity and lower off-target activity (Hu ...proteins has and will continue to dramatically increase the number of CRISPR applications. The first non-Cas9...formation of “sticky ends” rather than the blunt ends created by Cas9. Cas12a  displays lower off-target editing...) system fuses Cas13b to RNA deaminase ADAR2 to create a specific RNA editor. Cas13 enzymes are advantageous...

  4. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    Published
    March 12, 2019, 1:37 p.m.
    ...significant knowledge gap in your field or even creating a new field. A knowledge gap is an impediment ...of experiments or a laundry list of molecules to screen has unclear utility. You cannot predict what will... have no hook. A battery of new assays for your screen result may or may not actually help. Ultimately...distinguish mechanistic questions from open ended screens and data collection. Organize your research vision...want to know the answer to your research problem. Creating a coherent unified story and model can help make...Consider going beyond the whiteboard. You may wish to create a sculpture or print a 3D model to better illustrate... His interests include the quality control of secretory proteins in the Endoplasmic Reticulum, optimization...

  5. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    Published
    July 25, 2024, 1:15 p.m.
    ...level of antigen in the sample. This figure was created with BioRender.com  Direct ELISAs A direct ... bind non-specifically to assay components and increase background staining. Direct ELISAs also lack the... antibody. Incorporating a secondary antibody increases the sensitivity of the assay, since multiple secondary... both primary and secondary incubation steps, increasing the workload and time commitment for users. This...antibodies for the capture and detection steps, which increases the specificity of the assay. The assay is also...antibody availability Longer protocolLess specificityIncreased chance of cross-reactivity Sandwich ... High specificityHigh sensitivity Longer protocolIncreased chance of cross-reactivityChallenging to develop...

  6. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    Published
    April 11, 2024, 1:15 p.m.
    ...they function in nature can inform us on how to create better recombinant vectors. In this blog post, ...provide tips on how we can harness those features to create broad host range recombinant vectors.  A plasmid... host range in plasmids? Not all plasmids were created equally. Only a handful of plasmids have been identified..., from broad host range plasmids can be used to create recombinant vectors that can replicate in different...vinelandii, Brevibacterium methylicum, Caulobacter crescentus, Desulfovibrio vulgaris, Erwinia carotovora, ..., Bordetella spp., Brucella spp., Caulobacter crescentus, Escherichia coli, Gluconacetobacter xylinus,...recombinant vectors can be modified further to increase the range of hosts in which proteins can be expressed...

  7. Addgene Stands with the Scientific Community

    Type
    Blog Post
    Published
    April 23, 2025, 1:30 p.m.
    ...limitations to facilities and indirect costs, and increased tariffs present an unprecedented challenge to ...to help research institutions maximize their increasingly limited resources: Preserving scientific assets...authenticated materials at a fraction of the cost of creating and verifying them independently — particularly..., and antibody production that labs may find increasingly difficult to maintain or access. These services...with industry and public and private partners to create additional pathways for critical scientific work...community has demonstrated remarkable resilience and creativity in the face of funding challenges. By strengthening...

  8. The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years

    Type
    Blog Post
    Published
    Jan. 8, 2014, 3:10 p.m.
    ...was Addgene's first distributed plasmid and was created in the lab of my thesis advisor, Dr. Bruce Spiegelman...requested item of 2005. shRNA - In 2006, we saw an increase in popularity of empty backbones for shRNA expression...requested. Ubiquitin - One particular gene we saw increased interest in from 2008 to 2009 was ubiquitin. The...popular with scientists, but 2012 was marked by increased deposits (and requests) of biosensors, optogenetics...and for so many different purposes that we had to create a CRISPR page to keep up with all the new plasmids...of plasmid sharing? Check out this infographic created by our own Addgenie, Laura Veckerelli. Click on...

  9. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ...Harvard. There’s an astounding number of ways to create chromosomal mutations in bacteria, so many that...of surrounding genes. However, there are ways to create scarless mutations, ones that don’t leave any undesired..., CRISPR-Cas9 technology has also aided in the creation of scarless mutations, both in stand-alone systems...systems (Jiang et al., 2013), and those which increase the efficiency of Lambda red (Pyne et al., 2015;... recombineering (Khetrapal et al., 2015). They created vectors for Lambda Red that contained one of eight...aeruginosa. These toxins were a mix of Type VI secreted effector toxins, toxin-antitoxin type toxins, ...Enterobacter cloacae, and Serratia marcescens and have created dozens of deletions as well as insertions with ...

  10. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    Published
    April 8, 2025, 1:15 p.m.
    ...also helps confirm that lanes are loaded evenly. Created with BioRender.com. Loading controls are even...normalized values for the two conditions are equal. Created with BioRender.com. This kind of normalization...must be addressed with separate controls (green). Created with BioRender.com. Researchers often rely ... of the assay is between the two dotted lines. Created with BioRender.com. To see why this S-shaped...protein abundance versus band intensity (right). Created with BioRender.com. On this gel, the protein...loading control that can be used for normalization. Created with BioRender.com. Assumption #1: The loading...protein approach. By eye, it’s difficult to see an increasing concentration of β-actin in panel A. Indeed, ...
Showing: 211 - 220 of 790 results