We narrowed to 932 results for: Gene

...human genes have counterparts in the rat (Gibbs, et al., 2004). Additionally, breakthroughs in gene modification...abundance of genes related to limb regeneration (Vieira et al., 2019). To study regeneration scientists ...during and after embryogenesis. Addgene has over 600 plasmids that contain Xenopus genes and a tool-kit ...models.  Check out Addgene’s collection of plasmids containing Rattus norvegicus genes! Mexican axolotl ...array of molecular tools, such as gene manipulation and mutagenesis techniques, are available to the scientific...world worked to characterize each gene’s function. Some key genetics research with Arabidopsis include... for scientists to study human disease-relevant genes in such a controlled environment displays how essential...

...deletion of one or several of these genes permits the expression of genes farther downstream in the construct... two tandem sets of genes aligned head-to-head and the deletion of one of the gene pairs. Brainbow 2.0...Brainbow uses a Cre/lox system to label neurons. Genes encoding the fluorescent proteins are organized ...organized in tandem with a single promoter, but only the gene directly following the promoter is expressed. Cre-mediated... which uses Cre to trigger the inversion of two genes aligned head-to-head. Another variation, Brainbow...located directly upstream of the fluorescent protein genes, Cre recombination is required before fluorescence...fluorescent proteins topic page Resources on Addgene.org Browse Addgene’s fluorescent protein collection Learn...

...you were cloning a gene into an expression vector, you would want the start of the gene to end up just downstream... 5’ of the start of the gene and a HindIII site just 3’ of the end of the gene. This way you can then ...leaving the 5’ end of your gene just downstream of the promoter and placing the gene in the proper orientation... multiple sites allows you to easily orient your gene insert with respect to the promoter. For example...restriction sites EcoRI, XhoI, and HindIII. To place your gene in the proper orientation downstream of the promoter...binds the plasmid to the new fragment thereby generating a complete, circular plasmid that can be easily...green, insert) are cut with restriction enzymes to generate compatible overhangs that allow them to bind. ...

...the puromycin resistance gene through a 2A peptide. The puromycin resistance gene enables selection of cells...to translate Cas9-2A and the Puromycin resistance gene as separate peptides. PX459 This is the vector backbone...had a point mutation in the puromycin resistance gene that made it less effective in certain cell lines...Resources on the Addgene Blog Browse Our CRISPR Posts Learn about Using CRISPR to Generate Knockouts Read...Purpose  SpCas9 This plasmid expresses Streptococcus pyogenes (Sp) Cas9 from a Cbh promoter. Cbh is a modified... the key component of most CRISPR systems and generates double strange breaks at target sites 5' of the...your 20 nucleotide guide sequence of choice (for generation of a gRNA that will target SpCas9 to the approrpriate...

...the sgRNAiBAR library covers every annotated human gene, it enables genome-wide screening with a high MOI...a stop cassette upstream of an effector/reporter gene. In cells where the stop cassette is removed, the...fish, and mice, by replacing the effector with a gene of interest in the cells you are studying. Read ...calcineurin-dependent response elements (CDREs) and drives gene expression. Because calcineurin is required for ...antifungals that target calcineurin. Current calcineurin gene reporters are based on the bacterial βgal assay,...Competition (SPARC) is a fully genetic tool for labeling just a fraction of a genetically-defined subset of cells...lentiviral vectors the iBARs can be detected by next generation sequencing.  In order to avoid a high false-positive...

...Cardiac Gene Transfer Superior to AAV1, AAV6, AAV7, and AAV8 in the Mouse and Rat. Human Gene Therapy,...toolkit for human gene therapy. Molecular Therapy: The Journal of the American Society of Gene Therapy, 14(...AAV1 AAV1 was the first viral vector approved for gene therapy (Issa et al., 2023). It may be a good choice...exposure or rAAV treatment, which can pose problems for gene therapy applications. Cross-reactivity of AAV serotypes... Various AAV Serotypes and Their Applications in Gene Therapy: An Overview. Cells, 12(5), 785. https:/...Injection in Mouse and Human Skeletal Muscle. Human Gene Therapy, 31(3–4), 233–240. https://doi.org/10.1089...type 5 to 2,3-linked sialic acid is required for gene transfer. The Journal of Biological Chemistry, 276...

...sensitizing gene cassettes. Thse vectors are engineered to replace the drug resistantance genes in the bacteria...were identified for 61 such diseases. Now single gene alleles have been identified for 4,163, over 50%...Lowe talked about his work on the 500+ human tRNA genes and, until now, we've overlooked regulatory fragments...may be involved in preventing silencing of self-genes. Dr. Mello presented a hypothesis that these pathways...involved in RNA based surveillance of repetitive gene families and provide a way to guide inheritance ... field. Resources at Addgene Browse Addgene's RNAi resources Browse Addgene's CRISPR resources Read ...countries in number of plasmid requests from Addgene. Addgene - Canton Nucleic Acids Forum 2015 from Joanne...

...Ehrhardt, A., et al., Episomal vectors for gene therapy. Curr Gene Ther, 2008. 8(3): p. 147-61. PubMed PMID... functional genes at the site of insertion and which can potentially promote tumorigenesis in vivo. The...fellow at Massachusetts General Hospital with research interests in the fields of gene editing and stem cells...non-integrating plasmid from blood cells with unique epigenetic and gene expression signatures. Cell Res, 2011. 21...The phenomenon has been recorded in past gene therapy trials and remains a safety concern in the clinical...clonal T cell proliferation in two patients after gene therapy for SCID-X1. Science, 2003. 302(5644): p...the chances of insertional mutagenesis, which is the integration of transgenes into the genome that can ...

...used for genome editing, activating gene expression, repressing gene expression, and much more. But there...engineering viruses. For example, an Acr gene was used to replace genes in the difficult-to-engineer Sulfolobus... Selection marker while engineering viruses Acr genes can be used as positive selectable markers in engineering...et al., 2018). Only viral particles that have the gene deletion could replicate when challenged with the...Experiment Read more about Optogenetic Control of CRISPR   Resources on Addgene.org Find CRISPR Plasmids ...Nme) (Pawluk et al., 2016). While Streptococcus pyogenes Cas9 (SpyCas9) is the most used and well studied...10.1126/sciadv.1701620 Additional Resources on the Addgene Blog First time CRISPR user? Check out this post...

...editing single genes is very useful, sometimes, you need to modify multiple genes to generate a cellular ...Nicole Gaudelli, the Associate Director and Head of Gene Editing Technology at Beam Therapeutics, used a ...In addition to stem cells the authors targeted 6 genes in primary human T cells using 8 selected ABE8s ...the reduction in protein expression of these six genes. All ABE8s edited more efficiently than ABE7.10 ... was the winner. It could simultaneously edit 3 genes (98.1%, 98.3%, or 98.6% efficiency, respectively... eighth generation of adenine base editors, called ABE8 (Gaudelli et al., 2020). The generated ABE8s needed...replaced S. pyogenes Cas9 in the ABE complex with PAM-variant Cas9 proteins: the S. pyogenes NG-Cas9 (PAM...