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TypeBlog PostPublishedFeb. 25, 2016, 3:30 p.m....next thing. If I made a mistake, I evaluated what lessons I had learned – call it “troubleshooting.” Trying...I asked some colleagues to share valuable life lessons they have learned during their scientific training... them in a blog post on Born to Science: Life Lessons from Scientific Training More and more voices ...
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Light Sheet Fluorescence Microscopy
TypeBlog PostPublishedJuly 16, 2015, 2:30 p.m....Praparaten. Leipzig: S. Hierzel. J.A Dent, A.G. Polson, M.W. Klymkowsky. (1989) Development, 105.61-74... Ross A, J. Hecksher-Sorensen, R. Baldock, D. Davidson. (2002) Science, 296, 541-545. Pubmed. A. Ertürk.... Kim, S. Kalyanasundaram, A.S. Andalman, T.J. Davidson et al. (2013) Nature, 497, 332-337. Pubmed. R.... -
The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data
TypeBlog PostPublishedJuly 16, 2020, 1:15 p.m....the statistical independence assumption of the Poisson process. This modification also eases the interpretation...orders. Our model factors the likelihood in our Poisson process into two components: 𝜃x(t) and 𝜓z(t)....contains an intractable integral of the product of a Poisson and multivariate log-normal likelihoods. Effects... -
Droplet Digital PCR for AAV Quantitation
TypeBlog PostPublishedJan. 16, 2020, 2:18 p.m....qPCR runs before a new one is needed. For these reasons, we are in the process of transitioning our titering...for the detection of low copy numbers. For this reason, it can be used in the detection of rare sequences...PMID: 24328707. PubMed Central PMCID: PMC3991984. Song, Neng, et al. "Detection of circulating Mycobacterium... -
Tips for arabidopsis transformation
TypeBlog PostPublishedOct. 25, 2018, 1:23 p.m....Arabidopsis is a fantastic model organism for many reasons, not the least of which is ease of transformation...a protocol for antibiotic selection, check out Harrison et al., 2006. Tips: Don’t plate seeds too densely... 24032037. PubMed Central PMCID: PMC3759027. 2 Harrison, S. J. et al. (2006) ‘A rapid and robust method... -
An Introduction to Adenovirus
TypeBlog PostPublishedOct. 3, 2023, 1:15 p.m....genes that apparently contribute to virulence (Tollefson et al., 1996) – cell death has been reported when...6301263. Servellita, V., Sotomayor Gonzalez, A., Lamson, D. M., Foresythe, A., Huh, H. J., Bazinet, A. ...10.1182/BLOOD-2007-02-062117. PMID: 17510320. Tollefson, A. E., Scaria, A., Hermiston, T. W., Ryerse, ... -
AAVs CREATed for Gene Delivery to the CNS and PNS
TypeBlog PostPublishedSept. 28, 2017, 2:01 p.m....gross comparison of AAV.PHP.eB and AAV.PHP.S to their parental vectors. To quantify this comparison, capsids...PubMed PMID: 28671695. 2. Deverman BE, Pravdo PL, Simpson BP, et al. Cre-dependent selection yields AAV variants... -
The PAM Requirement and Expanding CRISPR Beyond SpCas9
TypeBlog PostPublishedAug. 20, 2020, 2:30 p.m....Fuller CK, Cameron P, Fazzio TG, Zhu LJ, Wolfe SA, Sontheimer EJ (2018) NmeCas9 is an intrinsically high-fidelity.... Kim E, Koo T, Park SW, Kim D, Kim K, Cho H-Y, Song DW, Lee KJ, Jung MH, Kim S, Kim JH, Kim JH, Kim ...Topkar VV, Nguyen NT, Zheng Z, Gonzales APW, Li Z, Peterson RT, Yeh J-RJ, Aryee MJ, Joung JK (2015) Engineered... -
Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone
TypeBlog PostPublishedFeb. 18, 2020, 2:15 p.m....meeting who felt isolated at a conference for any reason was a Singleton. Figure 1: The Binning Singletons...Gorski, Mark O. Martin, Maureen Berg, Michael D. L. Johnson, Tiffany Lowe-Power, Eric Griffis, Janet Iwasa,...Dunivin, Jen Greenwich, Susanna Harris, Heather Hendrickson, Jonathan Jacobs, Mo Kaze, Christina Kellogg,... -
Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More
TypeBlog PostPublishedAug. 13, 2019, 12:58 p.m....vertebrate embryogenesis. To illustrate some of the reasons that zebrafish have been embraced as a model organism...examine the consequences of manipulating genes. One reason for the success of zebrafish as a model organism... 2016). One common and robust strategy uses a transposon called Tol2 to randomly integrate foreign DNA...