We narrowed to 897 results for: ANT

...the thousands of other scientists using pLKO.1 variants learn about those updates? This is a problem many...resources can be found in our Plasmids 101 blog series. Want to learn more about sharing your plasmids via Addgene...looking to streamline your lab management and you want the works: organizing reagent storage and inventory... plasmid features, and reference tables (i.e. antibiotic concentrations, commonly used primers). We also...

...repairing DNA double strand breaks – (HR). HR is the dominant homology-directed repair (HDR) pathway in mammalian...higher HR frequencies than quiescent cells. It is important to know that HR is often mutated in cancer cells...recognition sequence in the donor DNA, you can guarantee there won’t be re-targeting after HDR. If your...your cells. Enrich S-phase cells HR operates predominantly in S and G2 phase of the cell cycle, when a ...

... popular and useful viral vectors in the lab. Advantages of lentivirus include a large genetic capacity...Figure 2). The HIV genes that do remain are very important for viral production: Gag (structural precursor...transduced with a lentiviral vector containing an antisense sequence against the HIV-1 envelope. As of 2013...contain a deletion in the 3’ LTR that prevents aberrant activation of nearby genes. These safer vectors...

...activator (1a) or a repressor (1b). Scientists took advantage of these discoveries to create a new system for...mating two single haploid yeast fusion plasmid transformants into a diploid strain for screening), or arrayed...increase the stringency of your binding. And most importantly: Independently validate identified binding partners...ensure that your setup works. The last thing you want is to run your lovely library screen only to find...

... as pre-made viral preps. If you can’t (or don’t want to) make viral preps in your lab, there are custom...Titering After generating your lentivirus, you’ll want to infect your cells with the virus–the right amount...conditions to optimize include incubation time, antibiotic conditions, and amount of polybrene. (psst…didn...have a large number of cells to transduce, you may want to consider spinfection. Spinfection is the process...

...comparable (within r2=0.96) to that of NGS data. Advantages of ICE Determine Editing Efficiency and Types...  ICE uses Sanger sequencing data to produce quantitative, NGS-quality analysis of CRISPR editing - enabling...guide design tool, guide validation tool, and guaranteed editing with the CRISPR Gene Knockout Kit. Please...featured topic page xCas9: Engineering a CRISPR Variant with Pam Flexibility RNA Editing with Cas13 and...

... allows access to a larger number of willing participants. While fibroblasts obtained from aged patients...hiPSCs than age-matched fibroblasts [3]. These advantages make PBMCs the ideal source of somatic cells ... vectors are based on the Epstein-Barr Nuclear Antigen-1 (oriP-EBNA-1) system where each vector contains...OCT3/4, SOX2, KLF4, C-MYC, LIN28 and SV40 large T antigen (SV40LT). The method had a reprogramming efficiency...

...at a lower rate than proteins encoded by highly abundant, charged tRNAs. For example Tuller et al. found...ribosome will move quickly along regions calling for abundant, charged tRNAs but will stall at regions calling...! I should just choose the codon with the most abundant pool of charged tRNAs in my host organism for ...normally found at low abundance in E. coli. The advantage of producing additional tRNAs is that you can ...

...into AAV. NmeCas9 has the added advantage that it can be turned off by anti-CRISPR proteins. The Sontheimer...genome modification, but they did so without a substantial immune response or toxicity. More AAV-based CRISPR...Resources on the Addgene Blog Learn more about Cas9 Variants and the Expanded CRISPR Toolbox Read about CRISPR...

...These differences in recombinase efficiency are important to keep in mind when deciding whether to use the...common means to achieve ligand control is to fuse a mutant estrogen receptor (ER) ligand binding domain (LDB...recombinase. The high concentration of FLEx vectors guarantees that most cells are transfected with all labeling...versions can be used in these experiments. This elegant combination of Cre, FLP, and inducible systems ...

...that asbestos exposure remains the single-most important factor in developing mesothelioma. Asbestos, and...cells that can begin an immune response. This important biomarker can be used to distinguish between three...earlier, thus improving the patient’s prognosis significantly, but also to monitor those who may be at greater...be Used to Study Cancer Drug Resistance Plasmid Grant to Aid Cervical Cancer Screening in Ghana Additional...

...Cut-to-Mutation distance" affects editing efficiency. One important point to note is that Cas enzymes can continue...The size of the intended edit is the biggest determinant when selecting a type of donor. ssDNA templates...Easi-CRISPR for more details. References and resources Want to know more? Check out the additional resources...PLoS ONE 9(8); (2014). PubMed. Making designer mutants in model organisms. Peng, Y. et al. Development...

... monomers, although this can be prevented via antibiotic selection (Crozat, 2014). How to Detect Plasmid...CGE-LIF) for detection and can provide data on the quantity of various topologies and multimers present within...DH5α, HB101, NEB Stable, Stbl3, etc., are recA- mutant strains, and therefore should have reduced frequencies...plasmids causes instability: CoIE1 encodes a determinant essential for plasmid monomerization and stability...

...d microinject into blastocysts to be implanted in pseudopregnant female mice. If you obtained the desired...other homology-directed repair experiments, you’ll want the guide cut site to be as close as possible to...microinjection, i-GONAD is performed directly on pregnant female mice using a small incision that exposes...

...your antibody's purity? Check out our latest protocol video, "Coomassie Purity Stain of Recombinant Antibodies...

...For example, in bacteria, transposons can move antibiotic resistance genes between plasmids or from plasmids...is active in vitro as well as in vivo in yeast, plant, insect, and mammalian cells, including human cells...2021). Target site preference and host range are important to consider when selecting a transposon for a ... Sleeping Beauty and piggyBac, which is a big advantage over viral vectors (~5 kB payload for AAV and ...

...contrast, fluorescence imaging has emerged as the dominant light microscopy contrast technique in modern ...acquired all fluorophores throughout the sample are constantly exposed to light, not just those in the focal...for this to happen.Using red light has a second advantage: red light penetrates deeper into biological tissue...-time monitoring of neuronal activity across substantial 3D volumes. In addition to 2-photon microscopy...

...2!) decades that it's hard to summarize, but we wanted to mark the occasion by trying.  When we started...viral vectors, and in 2022 we added ready-to-use antibodies. Now, we think of ourselves as a repository for...keep an eye on our blog and social media. In the meantime, thank you all so much for being part of our community...

...Addgene's booth? Well, first, Addgenies! We'll have an antibody specialist, Amrita, and a viral vector specialist...tons of information about Addgene's ready-to-use antibodies, viral vectors, and, of course, plasmids! Plus...Scientific Writing" on Sunday, from 12 pm - 2 pm. If you want to learn about, and practice, principles of clear...

...approximate, two-dimensional map. The Baker Lab wanted to create a new fold that is not present in nature...design model at atomic resolution” (Kuhlman and Dantas et al., 2003). That made it a powerful proof of...Hassabis, and John Jumper! References Kuhlman, B., Dantas, G., Ireton, G. C., Varani, G., Stoddard, B. L....

... my assay at like 23:00, so I decided to make a giant snow angel for biologists in our building. Unfortunately...2022   Congratulations to all our #DeckTheLab contestants!           View this post on Instagram                     ... labs with everything from Falcon tube trees to giant paper mache Krampus performing routine lab maintenance...

...depositor, but it can also be valuable data to share in grant proposals or to demonstrate impact in annual reports...features of dashboards are (i) easy access to important numbers and trends, (ii) user interaction with...metrics!  About the Author Joachim Goedhart is an assistant professor at the Section of Molecular Cytology...

...colony to DNA extraction and quantification so that you can focus on what you want to do with your plasmid ... grades of DNA  Read about the various ways to quantify DNA Resources on Addgene.org Learn about our...

...also added AAV1 and AAV5 preparations of several variants of a new optogenetic tool called PdCO, deposited...offering AAV1 and AAV5 preparations of several variants of a new optogenetic tool called PdCO.  This is...bistable nature, it enables inhibition of synapses distant to the site of injection with high temporal precision...

...peek in case you missed them. July 2016 was a fantastic month for the Addgene blog. We achieved roughly...editing when compared to the most commonly used Cas9 variant, SpCas9. These properties, combined with other ...in the post, may make Cpf1 the preferred CRISPR variant going forward.   Special Delivery Fluorophore Targeting...

...been working with Seeding Labs to provide plasmid grants to researchers in developing countries. Today we...announce that we’ve selected our first two plasmid grant awardees: Drs Louis Bengyella and Kwabena O. Duedu...excited to discover what new and creative ideas applicants will propose for plasmids from the Addgene community...

...into next year we’d like to know what topics you want to learn more about. Our post on “Making Your Own...Finally, we are always looking for guest bloggers who want to share their research with the community, so email...Mentoring Support By Joanne Kamens Plasmids 101: Antibiotic Resistance Genes By Marcy Patrick Plasmids...

...fresh new friend: Antibody of the Day!  Figure 4: The plasmid, viral prep, and antibody of the day While...others may be less obvious — but every change is meant to make your experience on our website better so...

...programmable DNA-binding domains" publication.  If you want to see this new feature in action, click here to...plasmid of interest, which may help you identify relevant protocols and experimental data Find other related... an article that used a plasmid from Addgene, we want to hear about it so we can link to it in the "Articles...

...Product Management “I knew very early on that I wanted to be an artist/designer. But there were a few ...Campbell, and read about his "follow your bliss' mantra and never forgot it. Usually when I see inspiring...Designer On the job search “Always go for the job you want, even if you don’t think you’re qualified. The worst...