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  3. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...AID* into genomic sites of interests via CRISPR techniques. This tool-kit includes an array of FPs as well...

  4. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ...jRCaMP1) and mApple (jRGECO1a) are also available. Detection methods Many calcium reporters use a single fluorescent...

  6. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ...Gi-coupled receptor in transgenic mice. Nature Biotechnology 17:165–169 . https://doi.org/10.1038/6165 Roth...

  7. A Look at Addgene's QC Process

    Type
    Blog Post
    Published
    May 6, 2025, 1:15 p.m.
    ...dedicated scientists with expertise in sequencing technologies and bioinformatics. Through their efforts, the...

  8. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    Published
    Aug. 29, 2024, 1:15 p.m.
    ... enables rapid assessment of emerging CRISPR technologies. Cell Genomics, 4(3), 100519. https://doi.org...

  9. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    Published
    March 7, 2024, 2:39 p.m.
    ...genomic landscapes should be editable with CRISPR technology.     Fig. 1: SpCas9 has sparse PAM coverage...

  11. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    Published
    March 15, 2016, 2:30 p.m.
    ...of lentiviral vector titration methods."BMC biotechnology 6.1 (2006): 1. PubMed PMID: 16836756. PubMed...

  18. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ... development and improvement of CRISPR-based technologies over the past decade. CRISPR screens are easily...

  20. Antibodies 101: Flow Compensation

    Type
    Blog Post
    Published
    May 16, 2024, 1:15 p.m.
    ... immunotherapies. She is currently a research technician in Arlene Sharpe's lab at Harvard Medical School...

  21. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...Systematic profiling of conditional degron tag technologies for target validation studies. Nat Commun 13...

  22. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ...more difficult. I’ve learned that some of the technologies that helped us navigate a global pandemic can...

  24. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ...are distributed under the terms of the UBMTA to protect our depositors’ IP, but there may also be extra...

  26. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...Transgenic Analysis of Plants’, Bioscience, Biotechnology, and Biochemistry, 71(8), pp. 2095–2100. doi...

  28. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    .../ul) could still be eluted from the column, as detected by qPCR, but this did not reduce cloning efficiencies...

  30. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ...Indoor and Outdoor Air. Environmental Science & Technology Letters, 2(4), 84. https://doi.org/10.1021/ACS.ESTLETT...
Showing: 631 - 660 of 685 results