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Showing: 421 - 440 of 691 results

  1. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    Published
    Jan. 12, 2021, 2:15 p.m.
    ...side note, my guess is that people will not be exchanging business cards for awhile or ever so your LinkedIn... want to do: Don’t connect via LinkedIn with strangers. It’s a waste of time. People that don’t know ...

  2. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    Published
    Sept. 6, 2017, 1:01 p.m.
    ...ability to remove, reset and ligate mismatched overhangs, yielding scarless DNA assembly, and allowing ...mixture. The exonuclease removes mismatched 3’ overhangs, so that the region of homology doesn’t have to...

  3. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ...TRICK reporter mRNA this displacement leads to a change in the fluorescent signal: untranslated RNAs appear...experiments, it’s important to verify that these changes do not impact translation rate, as the authors ...

  4. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    Published
    Feb. 20, 2018, 2:30 p.m.
    ... The space was modern with clean bold colors of orange and white. The decor was very contemporary and ...hours. It was robust, capable of generating a wide range of insert sizes and also produced high yields of...

  5. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    Published
    Oct. 19, 2023, 1:15 p.m.
    ... the feature its characteristic T-shape. The arrangement of the B-B’ and C-C’ palindromic sequences determines... ITR sequences?  Next-generation sequencing and Sanger sequencing might fail to correctly read through...

  6. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ...were compared head-to-head for gene expression changes of non-target genes and RNA-seq of a nuclear (MALAT1...CAR–T cells was recently devised by the Yingxiao Wang lab.  The crux of the system is a set of heat-inducible...

  7. The MTA at Addgene

    Type
    Blog Post
    Published
    July 23, 2024, 2:17 p.m.
    ...or agreement that states the terms of use for a tangible research material that is shared between two parties... via an online process, though the process has changed a lot over the last twenty years! This has helped...

  8. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    Published
    Feb. 13, 2024, 2:15 p.m.
    .../fddsv.2022.952326Joubert, M. K., Deshpande, M., Yang, J., Reynolds, H., Bryson, C., Fogg, M., Baker, ...https://doi.org/10.1016/j.ejpb.2018.06.008Lu, RM., Hwang, YC., Liu, IJ. et al. Development of therapeutic...

  9. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type
    Blog Post
    Published
    Sept. 19, 2017, 1:20 p.m.
    ...awhile (check out this review), but the Bruchez and Tsang labs recently developed a photosensitizer composed... in the comments below!   References 1. He, J., Wang, Y., Missinato, M. A., Onuoha, E., Perkins, L. A...

  14. Antibodies 101: Isotypes

    Type
    Blog Post
    Published
    Oct. 19, 2021, 1:15 p.m.
    ...switching Isotype switching occurs when a B cell changes which heavy chain constant domain it pairs with...the course of an immune response since it also changes the effector function of the antibody. Isotype ...

  16. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    Published
    June 29, 2017, 1:06 p.m.
    ...easier to navigate. These practical and effective changes will give you the ability to find the plasmid with... community of scientists) for suggesting these changes, and the SnapGene and Addgene software development...

  17. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    Published
    May 25, 2017, 2:30 p.m.
    ... Nano-lantern color palette to include cyan and orange Nano-lanterns and showed that these three luminescent...eNL-based calcium sensor displaying a 500% signal change upon calcium binding. Suzuki et al. also envision...

  18. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ... is a Cas9 expression vector developed by Feng Zhang’s lab at the Broad Institute (Sanjana et al., 2014...PubMed PMID: 12595892. 4. Sanjana NE, Shalem O, Zhang F. Improved vectors and genome-wide libraries for...

  19. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    Published
    June 12, 2018, 11:40 a.m.
    ...Alexander Stark Bas van Steensel Richard Young Yi Zhang Leonard Zon  You!     Chromatin-related genes...chromatin after T lymphocyte receptor signaling. Zhao K, Wang W, Rando OJ, Xue Y, Swiderek K, Kuo A, Crabtree ...

  20. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    Published
    June 6, 2023, 1:15 p.m.
    ...experimental needs, and can transduce (infect) a wide range of cell types with a high efficiency. However, gamma-retrovirus...as the LTRs or structural proteins are not interchangeable between the two systems, other, more general...
Showing: 421 - 440 of 691 results