We narrowed to 705 results for: ANG
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TypeBlog PostPublishedAug. 11, 2020, 1:15 p.m....which have recognition sites in the ITRs. Regular Sanger sequencing typically fails to sequence through ...and high GC content, but some companies now offer Sanger sequencing conditions which can read through this...
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Summer SciComm Series: Master of SciComm
TypeBlog PostPublishedJuly 21, 2022, 1:15 p.m....past and chatting with individuals whose research ranged from wildlife conservation to abortion law to poetry...confluence of science and the world it hopes to change. Most researchers are aware of the importance of... -
RUBY-Red Siliques
TypeBlog PostPublishedMarch 1, 2022, 2:15 p.m....site linkages and a terminator. Image from He, Y., Zhang, T., Sun, H. et al. Hortic Res. (2020). I ... References and Resources References He, Y., Zhang, T., Sun, H. et al. A reporter for noninvasively... -
Viral Vectors 101: Transductions
TypeBlog PostPublishedApril 30, 2024, 1:15 p.m....virus to deliver nucleic acids? Sounds sort of dangerous at first suggestion! The good news is that viruses... the right safety considerations, they aren't dangerous for controlled lab usage. Using viral transductions... -
Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering
TypeBlog PostPublishedMarch 4, 2014, 4:49 p.m....: to understand how a mouse changes emotions, to see how a monkey changes decision making, to cure a human... -
Learning Technical Skills at a Lab Central Workshop
TypeBlog PostPublishedFeb. 20, 2018, 2:30 p.m.... The space was modern with clean bold colors of orange and white. The decor was very contemporary and ...hours. It was robust, capable of generating a wide range of insert sizes and also produced high yields of... -
Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**
TypeBlog PostPublishedSept. 19, 2017, 1:20 p.m....awhile (check out this review), but the Bruchez and Tsang labs recently developed a photosensitizer composed... in the comments below! References 1. He, J., Wang, Y., Missinato, M. A., Onuoha, E., Perkins, L. A... -
Pushing the Limits of DNA Assembly
TypeBlog PostPublishedSept. 6, 2017, 1:01 p.m....ability to remove, reset and ligate mismatched overhangs, yielding scarless DNA assembly, and allowing ...mixture. The exonuclease removes mismatched 3’ overhangs, so that the region of homology doesn’t have to... -
Visualizing Translation at the Single Molecule Level
TypeBlog PostPublishedAug. 1, 2017, 1:15 p.m....TRICK reporter mRNA this displacement leads to a change in the fluorescent signal: untranslated RNAs appear...experiments, it’s important to verify that these changes do not impact translation rate, as the authors ... -
What Good Citizenship Can Do for Reproducibility in Science
TypeBlog PostPublishedNov. 7, 2019, 2:18 p.m.... reagents readily available. What if that could change? Everyone in science needs both a job and funding...around 100 professional editors who produce a broad range of primary research and review journals across life... -
Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids
TypeBlog PostPublishedNov. 20, 2018, 8:17 p.m....bought out by another company and catalog number changes) the original identifier loses its meaning, making... Angela Abitua... -
Networking Follow-up: I Met a Person. Now What?
TypeBlog PostPublishedJan. 12, 2021, 2:15 p.m....side note, my guess is that people will not be exchanging business cards for awhile or ever so your LinkedIn... want to do: Don’t connect via LinkedIn with strangers. It’s a waste of time. People that don’t know ... -
Quantifying DNA? Here are Five DNA Quantification Methods to Consider
TypeBlog PostPublishedJuly 21, 2020, 1:15 p.m....However, the measurements are made in the visible range and can be read with a standard ELISA reader if ... Jennifer Tsang... -
Using CRISPR/Cas9 to Edit Disease Out of the Genome
TypeBlog PostPublishedJune 23, 2022, 1:15 p.m....Cas9 has been used to generate mutations in a wide range of organisms, but its potential to efficiently correct...the use of nanoparticles. [6]. References 1. Zhang B. 2021. “CRISPR/Cas gene therapy.” Journal of cellular... -
New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor
TypeBlog PostPublishedApril 7, 2022, 1:15 p.m....fusion protein binds to glutamate, conformational changes lead to an increase in fluorescence intensity from...properties such as kinetics, ∆F/F0, SNR, dynamic range, photostability, quantum yield, and expression. ... -
Predicting Adverse Reactions to Monoclonal Antibody Drugs
TypeBlog PostPublishedFeb. 13, 2024, 2:15 p.m..../fddsv.2022.952326Joubert, M. K., Deshpande, M., Yang, J., Reynolds, H., Bryson, C., Fogg, M., Baker, ...https://doi.org/10.1016/j.ejpb.2018.06.008Lu, RM., Hwang, YC., Liu, IJ. et al. Development of therapeutic... -
Viral Vectors 101: Inverted Terminal Repeats
TypeBlog PostPublishedOct. 19, 2023, 1:15 p.m.... the feature its characteristic T-shape. The arrangement of the B-B’ and C-C’ palindromic sequences determines... ITR sequences? Next-generation sequencing and Sanger sequencing might fail to correctly read through... -
The MTA at Addgene
TypeBlog PostPublishedJuly 23, 2024, 2:17 p.m....or agreement that states the terms of use for a tangible research material that is shared between two parties... via an online process, though the process has changed a lot over the last twenty years! This has helped... -
Hot Plasmids - January 2023
TypeBlog PostPublishedJan. 31, 2023, 2:15 p.m....were compared head-to-head for gene expression changes of non-target genes and RNA-seq of a nuclear (MALAT1...CAR–T cells was recently devised by the Yingxiao Wang lab. The crux of the system is a set of heat-inducible... -
Why and how to start studying chromatin (with Addgene's help)
TypeBlog PostPublishedJune 12, 2018, 11:40 a.m....Alexander Stark Bas van Steensel Richard Young Yi Zhang Leonard Zon You! Chromatin-related genes...chromatin after T lymphocyte receptor signaling. Zhao K, Wang W, Rando OJ, Xue Y, Swiderek K, Kuo A, Crabtree ...