Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 889 results for: ANT;

Showing: 421 - 440 of 889 results

  1. 2023 and 2024: Reflections and Looking Ahead

    Type
    Blog Post
    Published
    Jan. 23, 2024, 2:15 p.m.
    ...(IPI), in which Addgene will distribute recombinant antibodies (rAbs) developed at IPI. The first IPI ...excited to launch our Antibody Data Hub, an open-access repository for antibody validation data. We’ll...world. That included 2008 pooled libraries, 290 antibodies, 18,359 viral items, and 166,247 plasmids. We... our distributor partners, with the launch of antibodies in China and expansion of viral services available...

  2. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

    Type
    Blog Post
    Published
    July 29, 2014, 6:16 p.m.
    ...scale: Look at the mEmerald variants first, followed by the color variants. The mEmerald plasmids offer...Davidson lab prepares to shutter its doors. “We wanted to give all of these vectors to the scientific ... FSU. Mike is really the one who had been very adamant that we distribute to as many researchers as we...reagents. "We are proud to be able to bring such an important collection to the scientific community," says ...scientists, and the FSU team who have made this large, important plasmid deposit possible.   ...

  3. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    Published
    May 18, 2018, 7:32 p.m.
    ...findings can be predictably applied to future work. Importantly, no one should expect that every study should...who have complaints about the unreliability of antibodies. IPI is working to address this issue directly...directly by creating well characterised antibodies against all human extracellular proteins with community-...research tools. BenchSci collates information about antibodies while FPbase curates information about fluorescent...and use computer based analysis techniques, an important component of reproducibility is making sure that...figures. Cause #2: Hidden “negative” data We all want to be able to tell a good story when presenting ...journal’s perspective, probably not what most readers want to read. As Susanna Bachle points out in her talk...

  4. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    Published
    Jan. 16, 2025, 3:42 p.m.
    ...administered systemically, without causing disease? Recombinant adeno-associated viral vectors, or rAAVs, can...serotypes can be useful to evade neutralizing antibodies developed from prior natural exposure or rAAV...route of administration (Wu, Asokan, et al., 2006). Want to know if a capsid is a good option for your experiment...61779-370-7_6 Tabebordbar, M., Lagerborg, K. A., Stanton, A., King, E. M., Ye, S., Tellez, L., Krunnfusz.... Directed evolution of a family of AAV capsid variants enabling potent muscle-directed gene delivery ...., Gerfen, C. R., Schiller, J., Dudman, J. T., Hantman, A. W., Looger, L. L., Schaffer, D. V., & Karpova...Karpova, A. Y. (2016). A Designer AAV Variant Permits Efficient Retrograde Access to Projection Neurons. ...

  5. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...expression. Experimental output is measured and quantified. Created with BioRender.com.   Optogenetic...2020). Light-gated ion channels are useful when you want to activate or inhibit specific neurons without ...pathway. Photoswitches are great for teasing out important branch points along a signaling cascade that may... activated. Created with BioRender.com.   Important considerations for using optogenetic tools Once...optogenetics or biosensors in one system. There can be substantial overlap in the activation curves (Marshel et ... fidelity with the stimulus. For example, if you want to stimulate at a fast frequency without missing...can wreak havoc on your experiments, so it is important to understand the details of how each tool performs...

  6. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    Published
    July 28, 2016, 2:30 p.m.
    ...reporter-based) each come with their advantages and disadvantages. Sanger sequencing is simple and the...1B below). Alternatively, if an attB-attP pair is anti-aligned (sites facing opposite directions) on a ...from multiple primer pairs, one can infer the predominant state of registers within a sample using a metric...multiple registers. However, it is only semi-quantitative. It won’t tell you the distribution of states...states in a sample; instead, it will suggest the predominant state of registers in a sample based on a similarity...of inputs (see Figure S9 of our paper (1)). We anticipate that, depending on the context and application...

  7. ReFigure: Save Scientific Figures into Dashboards and Share Your Insights

    Type
    Blog Post
    Published
    Dec. 13, 2017, 3:20 p.m.
    ... our ReFigures on the therapeutic use of anti-CD47 antibodies in immuno-oncology. Funding agencies such... Cataloging a reagent’s performance as in this antibody ReFigure Analyzing replications as in this ReFigure... ReFigure to coherently save analyses with the relevant figures from a literature review. No more hunting... and good for science ReFigures are published instantly and are editable by the author at any time. Insights...preprints, data, software, micropublications) in grant proposals. ReFigures are one such research product...

  8. Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics

    Type
    Blog Post
    Published
    June 11, 2019, 1:16 p.m.
    ...ve realized my figures are just as important, if not more important, than my writing. Initially I didn...design software, like Adobe Illustrator, and I didn’t want to pay for these programs either. But with a little...presentations, manuscripts, or social media. If you want to draw your own figures Google Drawings Google ...through your university or your public library. If you want to use pre-drawn images in your figures Biorender...New icons can be requested, although there’s no guarantee Biorender will create it. Additionally, you can...

  9. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    Published
    June 11, 2020, 1:34 p.m.
    ... have pushed the boundaries in neuroscience by granting targeted control over neuronal activity. While..., both techniques offer researchers different advantages and limitations.  The four main factors in which...studies, however, require a permanent intracranial implant to be surgically installed to allow for light delivery... delivery. Chemogenetics poses the advantage of being less invasive and more flexible as it relies on ...Invasiveness Mice require permanent intracranial implant Noninvasive Controlling Stimulation ExogenousEasily...controlled EndogenousNot easily controlled Unique advantages Mapping the brainDissecting circuits Studying...

  10. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    Published
    July 27, 2021, 1:15 p.m.
    ...direct, specific and sensitive detection of the plant hormones strigolactones By Fabienne Furt Current... detect strigolactones (SLs) are costly, non quantitative or require heavy optimization. Claudia Vickers...fluorescence intensity, it provides a direct and quantitative detection of SLs. The authors also showed that... known to trigger seed germination of parasitic plants, this approach was also used to build and validate...Qi's lab, allows simultaneous gene activation in plants.  FnCas12a, unlike other Cas12a nucleases identified...in moderate thermophiles.  These evolved SpCas9 variants have improved activity on NAG PAMS and reduced...

  11. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    Published
    Feb. 21, 2023, 2:15 p.m.
    ...ever been the target of an ‘-omics’ study or is resistant to specific compounds, links to publication references...cross-references with cell line catalogs and collections. Importantly, Cellosaurus also identifies problematic cell... the most common contaminator line.  For those wanting to access Cellosaurus programatically, their API... helpful information on the known features and relevant literature, or to decide which line is best for...scientists all over globe, with some surprising, and important, findings being shared. For instance, recently...around! If you’re excited about this resource and want to learn more, check out the Cellosaurus FAQ! References...

  12. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    Published
    June 30, 2015, 2 p.m.
    ... both of these cases, transposons combine the advantages of viruses and naked DNA while eliminating some...therapy - avoiding the use of viruses is also important for social and regulatory reasons. 1.) The transposon...Zsuzsanna Izsvak’s lab screened many SB tranposase variants for hyperactivity in mammalian cells. Their efforts...transposase (as determined by transposition assays of antibiotic-resistance genes conducted in HeLa cells). In...inducible expression vectors were developed into 16 variants with different selection markers (blasticidin,...as a nonviral vector for CD19-directed chimeric antigen receptor (CAR) therapy. SB is a valuable tool and...

  13. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

    Type
    Blog Post
    Published
    Sept. 18, 2018, 1:33 p.m.
    ...reporter can also quantify Cas9 cleavage, thus exhibiting its versatile utility in quantifying multiple gene...versatile in vivo fluorescent reporter for rapid quantification of base-editing and Cas9 cleavage activity ...several cell types is the lack of a rapid and quantitative editing assay. To help accelerate the expansion...plasmid that allows for rapid fluorescence-based quantification of base editors that utilize dCas9 fused to...frame-shift mutation within the mCherry reporter. Quantification of editing efficiencies simply involves tracking...

  14. Plasmid Cloning by PCR

    Type
    Blog Post
    Published
    March 29, 2016, 2:30 p.m.
    ...restriction sites are present in a given sequence. You want to choose enzymes that: Do not cut within your...Next, we need to examine the DNA sequence that we want to amplify and design primers that will bind to ...primer design: Because we are cloning an ORF, we want to clone from the start codon (ATG) to the stop ...reaction Run PCR to amplify your insert DNA. It is important to use a high fidelity polymerase to minimize .... The fidelity of the polymerase becomes more important the longer the expected PCR product is. You should...used. No matter which polymerase you use, it is important that you sequence the final product. Additional...

  15. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    Published
    Oct. 29, 2015, 2:30 p.m.
    ...treated a tetracycline resistant plasmid, pSC101, and a newly developed kanamycin resistant plasmid, pSC102,...with EcoRI and selected for E. coli transformants that were resistant to both. When this proved successful...generic term for any extrachromsomal hereditary determinant”. His proposal, however, was basically ignored...was the tool of choice for molecular biologists wanting to study bacterial genetics. This all changed thanks...

  16. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ...Imaging by Coat protein Knock-off) instead takes advantage of the ribosome’s movement. When the ribosome ... reporter. Delayed translation is known to be important in development, and Halstead et al. used TRICK...mRNA and keeps it in a single field of view for constant tracking. You can then directly monitor multiple...that can recruit GFP fused to the SunTag-specific antibody scFv. Each time the reporter is translated, the...second. They also explored the effects of 5’ UTR variants in translation of cell cycle protein Emi1, showing...and 3’ UTR. Before beginning experiments, it’s important to verify that these changes do not impact translation...

  17. Addgene Depositors Get More Citations

    Type
    Blog Post
    Published
    April 20, 2017, 2:30 p.m.
    ...This post was contributed by guest bloggers Samantha Zyontz and Neil Thompson from the MIT Sloan School...pulled the journals from 2005 to 2015 in areas most relevant to plasmids: Genetics & Heredity, Cell Biology...after controlling for journal. And so, while our quantitative analysis cannot rule out all other explanations...along the way. Many thanks to our guest bloggers, Samantha Zyontz and Neil Thompson. Special thanks to Addgenies...for helping compile the data used in this work. Samantha Zyontz is a Ph.D. candidate at the MIT Sloan School...ssrn.com/author=1232466.     Neil Thompson is an Assistant Professor of Innovation and Strategy at the MIT...

  18. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    Published
    July 17, 2018, 12:23 p.m.
    ...other professors at the University of Barcelona wanted to develop strategies to make our classes more ...thinking about how to solve this problem. I didn’t want to face another group of sleepy students at 8 a....engaging card game could help me get the engagement I wanted. I therefore started planning a game that would...following lessons. And let me tell you more, they had brilliant ideas on how to solve many therapeutic and pre-clinical...educational nature of this material are welcome and if you want to receive our modifiable powerpoint file, please...University of Barcelona. He is also founder and consultant at FRESCI particularly interested in advanced...

  19. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    Published
    March 1, 2018, 2:11 p.m.
    ...us take comfort in the fact that it’s often not quantity, but quality that really matters. Well, it turns...another example, neuronal gene expression by an anterograde AAV was shown to drop off drastically with lower...concentrating your AAV above your desired titer and want to dilute it, err on the side of caution when adding...References 1.Clark, K. Reed. "Recent advances in recombinant adeno-associated virus vector production." Kidney...PMID: 11841606. 2. Schultz BR, Chamberlain JS. Recombinant Adeno-associated Virus Transduction and Integration...PMC2574934. 3. Zingg, Brian, et al. "AAV-mediated anterograde transsynaptic tagging: mapping corticocollicular...

  20. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    Published
    Sept. 12, 2019, 1 p.m.
    ...-AAV-syn-jGCaMP7c variant 1513-WPRE (105321-AAV1) pGP-AAV-syn-FLEX-jGCaMP7c variant 1513-WPRE (105322-...vectors to more serotypes. We currently package four variants of these biosensors, which are optimized for specific...relative to baseline fluorescence, as the GCaMP7f variant, and is approximately 5-fold brighter than GCaMP6s...GCaMP6s. jGCaMP7f is the fastest responding variant. jGCaMP7b exhibits the brightest resting fluorescence...fluorescence from inactive neurons is reduced. This variant has an approximately 12-fold increase in brightness...
Showing: 421 - 440 of 889 results