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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 808 results for: ARC

Showing: 421 - 440 of 808 results

  1. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type
    Blog Post
    Published
    July 10, 2014, 7:17 p.m.
    ...engineer and scientist, I like to envision that my research will have a direct impact on healthcare and the...result, I have occasionally pondered translating my research into a startup company. However, like many like-minded...incorporated into Novophage Therapeutics, Inc. in March 2009 and tested in the “market” by competing in ...

  2. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ...Colorful CRISPR technologies are helping researchers visualize the genome and its organization within...low resolution and can’t be used in vivo. Some researchers have used fluorescently tagged DNA-binding proteins...CRISPR. Early CRISPR labeling techniques allowed researchers to visualize nearly any single genomic locus,...

  3. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

    Type
    Blog Post
    Published
    April 23, 2018, 2 p.m.
    ... podcast segment CRISPR and RNAi have helped researchers alter DNA sequence and RNA expression - but what... Yeast Transcription Factors Podcast Segment Researchers from the El-Samad lab recently deposited plasmids...that this level of control will enable future researchers to better simulate physiological gene expression...

  4. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ...out plenty of roles just the way they are, the research antibodies in your refrigerator often need a little...are kind of hard to see. To solve this issue, researchers conjugate various labels to antibodies that produce...conjugation, which is commonly used for labeling research antibodies. Lysine residues are found throughout...

  5. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ...of the different steps in RNA extraction. Researchers at the NIH in the mid 1980’s set out to develop...with chloroform as in the above no-kit method.  Researchers have also developed ways to “stabilize” RNA within...gene expression. There are several things that researchers can do to combat this issue. First and foremost...

  6. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...recently identified Cas12f systems from uncultivated archaea are much smaller and would be ideal for such AAV...system (CasMINI) engineered from the uncultivated archaea Cas12f (Un1Cas12f1, or Cas14a1) system. The CasMINI...editing efficiency. The lab also optimized the architecture and amino acid sequence of the prime editor,...

  8. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    Published
    Jan. 31, 2019, 1:32 p.m.
    ...Visit our cancer research plasmids and resources page  Find plasmids for your research Learn about Addgene's...this process is not fully understood, a team of researchers lead by Takayuki Kogure (Kogure et al., 2011)...

  9. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    Published
    Nov. 21, 2019, 1:53 p.m.
    ... long tenure at Addgene. I would work with our research team and software engineers to update our Lab ...engineer that you want the LIMS software to create a barcode for your plate, you will get in trouble if you ...given container Whether or not the container was barcoded (this is pretty Addgene specific) The types or...

  10. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...as part of its natural viral life-cycle, and researchers have adapted the components for use in genome...-lox? The Cre/lox system is a well-established research tool, especially in the field of mouse transgenics...parallel. Have you used the Cre-lox system in your research? Do you have any tips or tricks, or clever cre-dependent...

  11. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    Published
    July 23, 2020, 1:20 p.m.
    ..., Doxzen KW, Ma E, Liu J-J, Knott GJ, Edraki A, Garcia B, Amrani N, Chen JS, Cofsky JC, Kranzusch PJ, .../10.3390/v10120695 Pawluk A, Amrani N, Zhang Y, Garcia B, Hidalgo-Reyes Y, Lee J, Edraki A, Shah M, Sontheimer... on Addgene.org Find CRISPR Plasmids for Your Research Interested in light-inducible Cas9 activity? Check...

  12. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    Published
    June 25, 2020, 1:15 p.m.
    ... doesn’t mean you’ve failed. “I got a job as a research assistant in a lab at the university that I went...choice, but by circumstance. She was working as a research and technical assistant, when her life changed...often under a lot of pressure to publish their research and are not so keen on sharing their results in...

  14. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    Published
    July 11, 2019, 1:26 p.m.
    ...provide researchers with useful and adaptable tools to perform basic and preclinical research. For scientists...Inbred and outbred strains Mouse strains used in research can fall under one of two broad classifications...

  15. Tips for Technical Support Calls

    Type
    Blog Post
    Published
    Sept. 20, 2016, 2:39 p.m.
    ... the reagents available at Addgene come from researchers, and the vast majority of our plasmids and pooled...it, there’s a reason nearly 70% of all internet searches are done via Google. The “Let me Google that for...questions, consider using Google Scholar, which searches scientific articles, patents, and even some doctoral...

  16. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ...CRISPR toolbox. When Cpf1 was first discovered, researchers wondered if it could overcome some of Cas9’s ...templates should remove the PAM sequence. Many research and clinical applications require HDR over NHEJ...possibility has not been experimentally confirmed. Researchers have also not yet characterized the potential...

  18. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    Published
    June 5, 2014, 3:32 p.m.
    ... pretty good. We started doing extracurricular research on some of them to see how feasible they were,... for us? After about a month of discussion and research and lots of conversation, we hit upon the idea... They invested in us this summer – $30,000 for research and another $30,000 in mentorship and business...

  19. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    Published
    March 28, 2018, 6:52 p.m.
    ...d like to modify. To tackle the PAM problem, researchers have engineered alternative Cas9s binding distinct...this case, NNGRRT and NNNNGATT, respectively. Researchers also engineered SpCas9 variants with alternative...fused to third generation cytidine base editor architecture (BE3) slightly improves NGG PAM editing efficiency...

  20. Common Injection Routes in Mice

    Type
    Blog Post
    Published
    Jan. 22, 2025, 9:42 p.m.
    ...drugs, DNA, viral vectors, and viruses. All researchers must go through the proper basic training prior...commonly administered through the tail vein — many researchers will simply refer to these as “tail vein injections...feeding tube can be flexible or metal, depending on researcher preferences. It sounds complicated but has a ...
Showing: 421 - 440 of 808 results