We narrowed to 517 results for: ATO

1. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

   Type

   Blog Post

   Published

   July 23, 2020, 1:20 p.m.

   ...Peptides Decreases CRISPR/Cas9 Toxicity in Human Hematopoietic Stem Cells. Molecular Therapy - Methods & Clinical...

2. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

   Type

   Blog Post

   Published

   May 7, 2020, 1:15 p.m.

   ...editing, the researchers tested ABE8 in CD34+ hematopoietic stem cells. ABE8 efficiently edited the fetal...

3. Overcoming the Challenges of Lentiviral Production

   Type

   Blog Post

   Published

   Aug. 7, 2018, 12:31 p.m.

   ...systems such that it no longer requires the viral transactivator, Tat. For more information on the different...

4. Popular Retroviral Vectors and Their Uses in Scientific Research

   Type

   Blog Post

   Published

   July 24, 2018, 12:51 p.m.

   ...Destination vectors. These vectors can induce combinatorial constitutive or inducible gene overexpression...

5. Adenoviral Vector Production and Troubleshooting

   Type

   Blog Post

   Published

   June 14, 2018, 11:44 a.m.

   ...medicine (2017).   1st Gen AdVs are stripped of regulatory genes E1 and E3. Without these genes, AdVs cannot...

6. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

   Type

   Blog Post

   Published

   Aug. 20, 2019, 12:31 p.m.

   ...general consideration for behavioral testing in a laboratory, a high level of experimental control is required...

7. CRISPR in the Clinic

   Type

   Blog Post

   Published

   Nov. 2, 2023, 1:15 p.m.

   ... muscular dystrophy, cardiovascular disease, inflammatory diseases, blindness, and diabetes. Directions...

8. Viral Vectors 101: Chemogenetics

   Type

   Blog Post

   Published

   July 11, 2023, 1:15 p.m.

   ...PSAMs PSAMs PSAMs, or pharmacologically selective actuator modules, were developed with the goal of creating...

9. Viral Vectors 101: Virus Safety

   Type

   Blog Post

   Published

   June 22, 2023, 1:15 p.m.

   ..., some adenovirus strains are known to cause respiratory diseases in humans, earning this virus a BSL-...

10. Plasmids for Endogenous Gene Tagging in Human Cells

    Type

    Blog Post

    Published

    April 6, 2017, 1:02 p.m.

    ...specific, individual proteins under endogenous regulatory control. But even with the advent of CRISPR/Cas9...

11. Plasmids 101: The Wide World of Natural Plasmids

    Type

    Blog Post

    Published

    Oct. 8, 2024, 1:15 p.m.

    ...Auriol, P., Vogel, T. M., & Simonet, P. (2001). Laboratory-scale evidence for lightning-mediated gene transfer...

12. Making CRISPR Plasmids Using Fragmid

    Type

    Blog Post

    Published

    Aug. 29, 2024, 1:15 p.m.

    ...Plasmid pRDB_053 (for transgenic flies; gypsy insulator sequences; white + selectable marker; attB site...

13. 5 Tips for Troubleshooting Viral Transductions

    Type

    Blog Post

    Published

    Aug. 11, 2016, 2:23 p.m.

    ...sensitive to the toxicity of polybrene (e.g., hematopoietic or primary cells), and has been shown to increase...

14. Hot Plasmids and Viral Preps - January 2021

    Type

    Blog Post

    Published

    Jan. 5, 2021, 2:15 p.m.

    ...AAV9. Find viral preps here pAAV-hSyn-Cre-P2A-dTomato is now available in AAV1 and AAV8, as well as AAV5...

15. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type

    Blog Post

    Published

    Oct. 22, 2019, 12:20 p.m.

    ... center of the poster in a large font. Hence, spectators can read the text from many feet away and immediately...

16. Engaging with science and society at pgEd

    Type

    Blog Post

    Published

    Sept. 20, 2018, 12:17 p.m.

    ... in genetics research, their applications and regulatory policies; keeping tabs on the conversation about...

17. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type

    Blog Post

    Published

    July 14, 2016, 2:30 p.m.

    ...barely fits into AAV when you add in a gRNA and regulatory elements. To solve this problem, the Zhang lab...

18. Gaining Leadership Skills Volunteering at a Professional Organization

    Type

    Blog Post

    Published

    Aug. 6, 2015, 2:30 p.m.

    ...the work I agreed upon (just as I would in my laboratory work). Committee members have to work professionally...

19. Experimenting in an Open Source lab: from CRISPR to Cats

    Type

    Blog Post

    Published

    July 23, 2015, 2:30 p.m.

    ...trying to explore the links between genetics and anatomy, which are not yet fully understood. We attempt...

20. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type

    Blog Post

    Published

    Jan. 21, 2015, 1:30 p.m.

    ...graduate students since 2009. Dora is also the creator of the “Finish Your Thesis” online course.  To ...