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We narrowed to 798 results for: Arc

Showing: 421 - 440 of 798 results

  1. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...recently identified Cas12f systems from uncultivated archaea are much smaller and would be ideal for such AAV...system (CasMINI) engineered from the uncultivated archaea Cas12f (Un1Cas12f1, or Cas14a1) system. The CasMINI...editing efficiency. The lab also optimized the architecture and amino acid sequence of the prime editor,...

  3. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ...of the different steps in RNA extraction. Researchers at the NIH in the mid 1980’s set out to develop...with chloroform as in the above no-kit method.  Researchers have also developed ways to “stabilize” RNA within...gene expression. There are several things that researchers can do to combat this issue. First and foremost...

  4. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    Published
    Nov. 21, 2019, 1:53 p.m.
    ... long tenure at Addgene. I would work with our research team and software engineers to update our Lab ...engineer that you want the LIMS software to create a barcode for your plate, you will get in trouble if you ...given container Whether or not the container was barcoded (this is pretty Addgene specific) The types or...

  5. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...as part of its natural viral life-cycle, and researchers have adapted the components for use in genome...-lox? The Cre/lox system is a well-established research tool, especially in the field of mouse transgenics...parallel. Have you used the Cre-lox system in your research? Do you have any tips or tricks, or clever cre-dependent...

  6. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    Published
    July 23, 2020, 1:20 p.m.
    ..., Doxzen KW, Ma E, Liu J-J, Knott GJ, Edraki A, Garcia B, Amrani N, Chen JS, Cofsky JC, Kranzusch PJ, .../10.3390/v10120695 Pawluk A, Amrani N, Zhang Y, Garcia B, Hidalgo-Reyes Y, Lee J, Edraki A, Shah M, Sontheimer... on Addgene.org Find CRISPR Plasmids for Your Research Interested in light-inducible Cas9 activity? Check...

  7. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    Published
    June 25, 2020, 1:15 p.m.
    ... doesn’t mean you’ve failed. “I got a job as a research assistant in a lab at the university that I went...choice, but by circumstance. She was working as a research and technical assistant, when her life changed...often under a lot of pressure to publish their research and are not so keen on sharing their results in...

  9. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    Published
    July 11, 2019, 1:26 p.m.
    ...provide researchers with useful and adaptable tools to perform basic and preclinical research. For scientists...Inbred and outbred strains Mouse strains used in research can fall under one of two broad classifications...

  10. Tips for Technical Support Calls

    Type
    Blog Post
    Published
    Sept. 20, 2016, 2:39 p.m.
    ... the reagents available at Addgene come from researchers, and the vast majority of our plasmids and pooled...it, there’s a reason nearly 70% of all internet searches are done via Google. The “Let me Google that for...questions, consider using Google Scholar, which searches scientific articles, patents, and even some doctoral...

  11. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ...CRISPR toolbox. When Cpf1 was first discovered, researchers wondered if it could overcome some of Cas9’s ...templates should remove the PAM sequence. Many research and clinical applications require HDR over NHEJ...possibility has not been experimentally confirmed. Researchers have also not yet characterized the potential...

  13. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    Published
    June 5, 2014, 3:32 p.m.
    ... pretty good. We started doing extracurricular research on some of them to see how feasible they were,... for us? After about a month of discussion and research and lots of conversation, we hit upon the idea... They invested in us this summer – $30,000 for research and another $30,000 in mentorship and business...

  14. Common Injection Routes in Mice

    Type
    Blog Post
    Published
    Jan. 22, 2025, 9:42 p.m.
    ...drugs, DNA, viral vectors, and viruses. All researchers must go through the proper basic training prior...commonly administered through the tail vein — many researchers will simply refer to these as “tail vein injections...feeding tube can be flexible or metal, depending on researcher preferences. It sounds complicated but has a ...

  15. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ...depositing them in Addgene’s repository. Other researchers, eager to try this new technology, were quick...the CRISPR community?  Addgene exists to help researchers share useful tools, and as such, we’re always...collection, we’ve shared almost 300,000 plasmids with researchers all over the world. That’s an average of 27,272...

  16. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...devising their tagging strategy, another group of researchers was working on isolating monoclonal antibodies...identified oncogenes and was a target of intense research. Evan, et al. hoped that their new monoclonals...producing anti-Myc [9E10] as a recombinant antibody. Researchers first sequenced the variable domains of Myc1-...

  17. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    Published
    March 28, 2018, 6:52 p.m.
    ...d like to modify. To tackle the PAM problem, researchers have engineered alternative Cas9s binding distinct...this case, NNGRRT and NNNNGATT, respectively. Researchers also engineered SpCas9 variants with alternative...fused to third generation cytidine base editor architecture (BE3) slightly improves NGG PAM editing efficiency...

  18. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    Published
    May 23, 2019, 12:26 p.m.
    ...So you’ve done the research, gathered up your data into an exciting story, and are ready to present your...all will be familiar with the specifics of your research. Try to avoid jargon in this initial introduction...data they may ask the audience to not share their research on social media. Use the hashtag if you are live-tweeting...

  19. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ... essential part of being able to continue your research. As an academic postdoc, you’re often required... at once at the end of February or beginning of March, and the jobs are mixed into the general careers...developmental biology and is applying this towards researching neurodegenerative diseases.   Additional resources...

  20. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...Jesse Bloom’s lab at the Fred Hutchinson Cancer Research Center developed a pooled plasmid library encompassing...plasmid encodes the mutated spike protein and a barcode, and is designed so that the SARS-CoV-2 spike RBD...encapsulating a single enzyme. Compared with previous research, which required protein compartments to co-localize...
Showing: 421 - 440 of 798 results