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  2. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    Published
    July 18, 2019, 12:55 p.m.
    ...The C. elegans community has always emphasized the need for open science and collaboration. The field already...transgene control in Caenorhabditis elegans." Proceedings of the National Academy of Sciences 115.15 (2018...

  3. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    Published
    June 27, 2017, 1:03 p.m.
    ...laboratory may have already created the sensor that you need. FRET biosensors designed to measure specific small...insertion within green fluorescent proteins." Proceedings of the National Academy of Sciences 96.20 (1999...

  4. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    Published
    Aug. 29, 2024, 1:15 p.m.
    ...promoters, N’ and/or C’ terminus, and selection markers. Need fewer components? A spacer can be inserted instead...backbone and genes contain only the Bbsl cut sites needed for the Fragmid cuts, clone in the appropriate ...

  5. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    Published
    April 6, 2023, 3 p.m.
    ...dollars on a new secondary antibody if you don’t need it.  If, on the other hand, your target is expressed...approach, that is simple - but what about when you need to use an indirect (primary and secondary antibody...

  6. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ... easier for you to find and use the plasmids you need. Here's what you'll find in this post: Epigenetic... regulators or chromatin-modifying enzymes. The speed and flexibility of the single-pot system will allow...

  7. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    Published
    Oct. 22, 2019, 12:20 p.m.
    ... you can show off your work at a conference, you need to first make your poster. While a poster generally... to verbally communicate your work, so you don’t need every little detail on the board.  Poster layout...

  8. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ...one RNA rather than the two (tracrRNA and crRNA) needed by Cas9 for cleavage. Now, two new studies show...given that Cpf1 gRNAs are also shorter than those needed for Cas9 (42 nt vs 100 nt.) However, the newly ...

  9. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    Published
    July 23, 2015, 2:30 p.m.
    ...locomotion, but our worms are constantly moving so we need good ways to monitor and track them. We have a number...microscopes, including an automated microscope but we need to develop the manual skills at the regular microscope...

  11. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    Published
    June 18, 2020, 1:15 p.m.
    ... reagents in three major steps - all without the need to find a llama farm! To find an optimally binding... Vlieger D, Corbett KS, Torres GM, Wang N, Van Breedam W, Roose K, van Schie L, Hoffmann M, Pöhlmann S...

  12. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ...protein you are trying to express How much protein you need   Your plans for downstream applications In this...than bacterial cells and growing conditions often need to be optimized. Yeast cells are also known for ...

  13. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    Published
    March 28, 2018, 6:52 p.m.
    ... xCas9 - here’s what we know so far! Why do we need PAM flexibility? IDT estimates that there is an ...Despite the progress we’ve made, there is still a need for and interest in enzymes with shorter PAM sequences...

  14. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    Published
    Sept. 12, 2017, 1:44 p.m.
    ...CHRM4 gene. When contamination is suspected, we proceed to de novo assembly of the unmapped reads, employing...interrogating the VGS data for small predetermined seed sequences unique to the capsid plasmid. Our recombination...

  15. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    Published
    Sept. 7, 2017, 12:17 p.m.
    ... cell clones with sterile truncated pipet tips. Indeed, an experienced operator can pick 48 clones from... your colonies are well separated. For this, you need to have some idea of the rate of growth of your ...

  16. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...mammalian cells, or in purified form, and didn’t need any added cofactors.      Figure 2: Fluorescence... and often, including works-in-progress still in need of further study and development like MagLOV. The...

  17. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...system – HaloTag and its PROTAC are all that is needed to facilitate degradation. HaloTag is also the ...tag does depend a lot on your unique experimental needs. If your protein’s function is easily perturbed ...

  18. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ...arm repair template (Fig. 1, Step 1). There is no need for PCR or cloning, as digestion of the destination...endogenous genes in human cells. To use CRISPaint, users need 3 vectors:  1) a gRNA vector that targets the gene...

  20. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...ve sold out. You’ve given up your intellectual freedom and integrity. (Not the case, as I’ve discovered...has a unique approach, and some might suit your needs better than others. Whether you decide to do an ...
Showing: 421 - 440 of 686 results