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We narrowed to 700 results for: EED

Showing: 421 - 440 of 700 results

  1. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    Published
    July 14, 2021, 1:15 p.m.
    ...variability. Once an animal has undergone a terminal bleed to acquire all of its serum, there is no more of...antibody composition available. If you’re going to need a large quantity of antibody for your experiment...

  2. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    Published
    Sept. 8, 2020, 2 p.m.
    ...creatine. In order to reach the brain, creatine needs to be transported from the blood and cross the blood...effector functions. It’s an important process that we need to understand so that we could possibly develop ...

  4. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    Published
    Nov. 24, 2015, 3:30 p.m.
    ...experimental manipulations, saving the time and money needed to develop new lines. Using nanobodies to create...experimental questions, but not having the tools needed to do so. Cepko lab researcher Jonathan Tang wanted...

  6. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...the stop codon is excised, and gene expression proceeds. One popular lox-stop-lox plasmid is from Tyler... introduce Cre into cells of interest. What if I need two separate recombination events? One potential...

  7. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    Published
    Aug. 29, 2024, 1:15 p.m.
    ...promoters, N’ and/or C’ terminus, and selection markers. Need fewer components? A spacer can be inserted instead...backbone and genes contain only the Bbsl cut sites needed for the Fragmid cuts, clone in the appropriate ...

  8. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    Published
    April 6, 2023, 3 p.m.
    ...dollars on a new secondary antibody if you don’t need it.  If, on the other hand, your target is expressed...approach, that is simple - but what about when you need to use an indirect (primary and secondary antibody...

  9. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ... easier for you to find and use the plasmids you need. Here's what you'll find in this post: Epigenetic... regulators or chromatin-modifying enzymes. The speed and flexibility of the single-pot system will allow...

  10. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    Published
    Oct. 22, 2019, 12:20 p.m.
    ... you can show off your work at a conference, you need to first make your poster. While a poster generally... to verbally communicate your work, so you don’t need every little detail on the board.  Poster layout...

  11. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    Published
    July 18, 2019, 12:55 p.m.
    ...The C. elegans community has always emphasized the need for open science and collaboration. The field already...transgene control in Caenorhabditis elegans." Proceedings of the National Academy of Sciences 115.15 (2018...

  12. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ... easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid...of H2B-FT consistently predicted the cell cycle speed across these different cell types. In one example...

  14. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    Published
    June 27, 2017, 1:03 p.m.
    ...laboratory may have already created the sensor that you need. FRET biosensors designed to measure specific small...insertion within green fluorescent proteins." Proceedings of the National Academy of Sciences 96.20 (1999...

  15. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ...one RNA rather than the two (tracrRNA and crRNA) needed by Cas9 for cleavage. Now, two new studies show...given that Cpf1 gRNAs are also shorter than those needed for Cas9 (42 nt vs 100 nt.) However, the newly ...

  16. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    Published
    July 23, 2015, 2:30 p.m.
    ...locomotion, but our worms are constantly moving so we need good ways to monitor and track them. We have a number...microscopes, including an automated microscope but we need to develop the manual skills at the regular microscope...

  17. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...mammalian cells, or in purified form, and didn’t need any added cofactors.      Figure 2: Fluorescence... and often, including works-in-progress still in need of further study and development like MagLOV. The...

  18. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...ve sold out. You’ve given up your intellectual freedom and integrity. (Not the case, as I’ve discovered...has a unique approach, and some might suit your needs better than others. Whether you decide to do an ...

  19. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...system — HaloTag and its PROTAC are all that is needed to facilitate degradation. HaloTag is also the ...tag does depend a lot on your unique experimental needs. If your protein’s function is easily perturbed ...
Showing: 421 - 440 of 700 results