We narrowed to 517 results for: Emb

1. The time and cost required to make a plasmid

   Type

   Blog Post

   Published

   March 23, 2023, 1:15 p.m.

   ... is a concern. The faster methods, like Gibson assembly, are typically more expensive, so you’ll have ...

2. Addgene: The First Twenty Years

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   Blog Post

   Published

   Dec. 19, 2024, 2:15 p.m.

   ...labhold” name. Our first plasmid request came on September 8, 2004, for pcDNA-p160MBP, while the first request...

3. Antibodies 101: Affinity Reagents

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   Blog Post

   Published

   April 14, 2022, 1:15 p.m.

   ...through mutation, allowing diabodies to be used for assembling protein nanostructures. Diabodies readily co-...

4. Transferable Skills Guide: Problem Solving

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   Blog Post

   Published

   Nov. 30, 2021, 2:15 p.m.

   ...both in and out of the lab. The next step is to remember that you have these skills when you start applying...

5. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

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   Blog Post

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   Aug. 5, 2021, 12:15 p.m.

   ...multiplexed imaging of neurotransmitter, calcium, and membrane voltage,” says Tian. Figure 5: Citrine-tagged...

6. CRISPRainbow and Genome Visualization

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   Blog Post

   Published

   Feb. 28, 2017, 3:30 p.m.

   ...GFP-dCas9 and the gRNA scaffold to enhance complex assembly, thereby decreasing the amount of background fluorescence...

7. Choosing the B(right)est Fluorescent Protein: Photostability

   Type

   Blog Post

   Published

   June 8, 2017, 2:30 p.m.

   ...in microdroplets in a water/oil emulsion, (ii) embedded in a gel, or (iii) attached to a substrate. These...

8. Summer SciComm: Preprints

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   Blog Post

   Published

   Aug. 16, 2022, 1:15 p.m.

   ...journal and share it more widely!  Not everyone embraces preprints as part of their scientific communication...

9. Gendered Innovations: Why Does Sex of the Cell Matter?

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   Blog Post

   Published

   July 21, 2016, 2:30 p.m.

   ...analysis for innovation and discovery. She is a member of the American Academy of Arts and Sciences and...

10. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

    Type

    Blog Post

    Published

    Dec. 16, 2015, 3:30 p.m.

    ... their ability to cut the EMX1 locus in human embryonic kidney (HEK) cells, 11 of these initial mutants...

11. The Future of Research Symposium Boston 2015

    Type

    Blog Post

    Published

    Oct. 20, 2015, 2:30 p.m.

    ...posing some of their own are the following panel members: Jonathan Dinman, Chair of the Cell Biology and...

12. New Tool for Lineage Tracing: The ClonTracer Library

    Type

    Blog Post

    Published

    Sept. 22, 2015, 2:30 p.m.

    ...library allows a researcher to label individual members of a starting population of cells with specifc,...

13. CRISPR in the Clinic

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    Blog Post

    Published

    Nov. 2, 2023, 1:15 p.m.

    ... in clinical trials includes Cas3 (the shredder member of the Cas family) as part of a cocktail therapy...

14. Viral Vectors 101: Virus Safety

    Type

    Blog Post

    Published

    June 22, 2023, 1:15 p.m.

    ...biosafety cabinet. When decontaminating liquid waste, remember that the percentage indicates the proportion of...

15. Antibodies 101: Choosing the Right Isotype

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    Blog Post

    Published

    April 6, 2023, 3 p.m.

    ..., G. P., & Fisher, S. K. (1993). Chapter 15 Postembedding Immunocytochemical Techniques for Light and ...

16. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

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    Blog Post

    Published

    Nov. 14, 2024, 2:15 p.m.

    ...splice sites. Nat Biotechnol. Published online November 5, 2018. doi:10.1038/nbt.4283 Zibitt MS, Hartford...

17. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type

    Blog Post

    Published

    Aug. 24, 2021, 1:15 p.m.

    ...your standard curve to plot to a linear equation (remember y = mx+b?). Figure 5: A standard curve ...

18. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

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    Blog Post

    Published

    July 23, 2020, 1:20 p.m.

    ... with Cas9 interferes and prevents the correct assembly of the crRNA-Cas complex (Zhu et al., 2019). Inhibiting...

19. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type

    Blog Post

    Published

    June 25, 2020, 1:15 p.m.

    ...Addgene as a DNA Sequencing Analyst intern. As a member of the Quality Control team, I help make sure the...

20. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

    Type

    Blog Post

    Published

    May 1, 2018, 1:57 p.m.

    ...considerations for Split-BioID It’s important to remember that with Split-BioID, and BioID, any protein ...