We narrowed to 514 results for: Emb
-
TypeBlog PostPublishedAug. 16, 2022, 1:15 p.m....journal and share it more widely! Not everyone embraces preprints as part of their scientific communication...
-
Antibodies 101: Affinity Reagents
TypeBlog PostPublishedApril 14, 2022, 1:15 p.m....through mutation, allowing diabodies to be used for assembling protein nanostructures. Diabodies readily co-... -
Transferable Skills Guide: Problem Solving
TypeBlog PostPublishedNov. 30, 2021, 2:15 p.m....both in and out of the lab. The next step is to remember that you have these skills when you start applying... -
Addgene: The First Twenty Years
TypeBlog PostPublishedDec. 19, 2024, 2:15 p.m....labhold” name. Our first plasmid request came on September 8, 2004, for pcDNA-p160MBP, while the first request... -
Choosing the B(right)est Fluorescent Protein: Photostability
TypeBlog PostPublishedJune 8, 2017, 2:30 p.m....in microdroplets in a water/oil emulsion, (ii) embedded in a gel, or (iii) attached to a substrate. These... -
CRISPRainbow and Genome Visualization
TypeBlog PostPublishedFeb. 28, 2017, 3:30 p.m....GFP-dCas9 and the gRNA scaffold to enhance complex assembly, thereby decreasing the amount of background fluorescence... -
Gendered Innovations: Why Does Sex of the Cell Matter?
TypeBlog PostPublishedJuly 21, 2016, 2:30 p.m....analysis for innovation and discovery. She is a member of the American Academy of Arts and Sciences and... -
Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9
TypeBlog PostPublishedDec. 16, 2015, 3:30 p.m.... their ability to cut the EMX1 locus in human embryonic kidney (HEK) cells, 11 of these initial mutants... -
The Future of Research Symposium Boston 2015
TypeBlog PostPublishedOct. 20, 2015, 2:30 p.m....posing some of their own are the following panel members: Jonathan Dinman, Chair of the Cell Biology and... -
New Tool for Lineage Tracing: The ClonTracer Library
TypeBlog PostPublishedSept. 22, 2015, 2:30 p.m....library allows a researcher to label individual members of a starting population of cells with specifc,... -
CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems
TypeBlog PostPublishedJuly 23, 2020, 1:20 p.m.... with Cas9 interferes and prevents the correct assembly of the crRNA-Cas complex (Zhu et al., 2019). Inhibiting... -
Meet an Addgenie: Insights into Grad School, Career Paths, and More
TypeBlog PostPublishedJune 25, 2020, 1:15 p.m....Addgene as a DNA Sequencing Analyst intern. As a member of the Quality Control team, I help make sure the... -
Viral Vectors 101: Virus Safety
TypeBlog PostPublishedJune 22, 2023, 1:15 p.m....biosafety cabinet. When decontaminating liquid waste, remember that the percentage indicates the proportion of... -
Antibodies 101: Choosing the Right Isotype
TypeBlog PostPublishedApril 6, 2023, 3 p.m...., G. P., & Fisher, S. K. (1993). Chapter 15 Postembedding Immunocytochemical Techniques for Light and ... -
Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)
TypeBlog PostPublishedAug. 24, 2021, 1:15 p.m....your standard curve to plot to a linear equation (remember y = mx+b?). Figure 5: A standard curve ... -
Corralling and Cornering the Goldilocks Antibody
TypeBlog PostPublishedAug. 9, 2022, 1:15 p.m....Zhuhao Wu and his colleagues! Dr. Wu and his lab members (like me!) are dedicated to creating a public and... -
CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9
TypeBlog PostPublishedNov. 14, 2024, 2:15 p.m....splice sites. Nat Biotechnol. Published online November 5, 2018. doi:10.1038/nbt.4283 Zibitt MS, Hartford... -
CRISPR in the Clinic
TypeBlog PostPublishedNov. 2, 2023, 1:15 p.m.... in clinical trials includes Cas3 (the shredder member of the Cas family) as part of a cocktail therapy... -
Engaging with science and society at pgEd
TypeBlog PostPublishedSept. 20, 2018, 12:17 p.m....’ve since returned to pgEd as a full-time staff member for the past three years. pgEd is a small team ... -
Overcoming the Challenges of Lentiviral Production
TypeBlog PostPublishedAug. 7, 2018, 12:31 p.m....dish can be easily applied to multiple dishes, remember that handling multiple dishes will take longer...